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Protein

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Gene

murA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell wall formation (PubMed:1512209). Adds enolpyruvyl to UDP-N-acetylglucosamine (PubMed:1512209, PubMed:20392080). Target for the antibiotic fosfomycin.2 Publications

Catalytic activityi

Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine.UniRule annotation2 Publications

Activity regulationi

Competitively inhibited by UDP-N-acetylglucosamine 3'-phosphate, with a Ki of 7 µM (PubMed:21445328). In vitro inhibited by covalent binding of fosfomycin and the fungal product terreic acid in the presence of substrate UDP-N-acetylglucosamine, with an inactivation rate constant of 102 M(-1)s(-1) for terreic acid (PubMed:20392080).2 Publications

Kineticsi

  1. KM=15 µM for UDP-N-acetylglucosamine1 Publication

    Pathwayi: peptidoglycan biosynthesis

    This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
    View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei91UDP-N-acetylglucosamineCombined sources1 Publication1
    Active sitei115Proton donorCombined sources1 Publication2 Publications1
    Binding sitei305UDP-N-acetylglucosamineCombined sources2 Publications1
    Binding sitei327UDP-N-acetylglucosamine; via carbonyl oxygenCombined sources1 Publication1

    GO - Molecular functioni

    • UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Source: EcoCyc

    GO - Biological processi

    Keywordsi

    Molecular functionTransferase
    Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
    LigandPyruvate

    Enzyme and pathway databases

    BioCyciEcoCyc:UDPNACETYLGLUCOSAMENOLPYRTRANS-MONOMER
    MetaCyc:UDPNACETYLGLUCOSAMENOLPYRTRANS-MONOMER
    BRENDAi2.5.1.7 2026
    UniPathwayi
    UPA00219

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.7UniRule annotation)
    Alternative name(s):
    Enoylpyruvate transferaseUniRule annotation
    UDP-N-acetylglucosamine enolpyruvyl transferaseUniRule annotation
    Short name:
    EPTUniRule annotation
    Gene namesi
    Name:murAUniRule annotation
    Synonyms:murZ
    Ordered Locus Names:b3189, JW3156
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11358 murA

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL1984
    DrugBankiDB00828 Fosfomycin

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001788701 – 419UDP-N-acetylglucosamine 1-carboxyvinyltransferaseAdd BLAST419

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei1152-(S-cysteinyl)pyruvic acid O-phosphothioketal1 Publication1

    Proteomic databases

    EPDiP0A749
    PaxDbiP0A749
    PRIDEiP0A749

    Interactioni

    Protein-protein interaction databases

    BioGridi4262438, 644 interactors
    DIPiDIP-48060N
    IntActiP0A749, 25 interactors
    STRINGi316407.85675983

    Chemistry databases

    BindingDBiP0A749

    Structurei

    Secondary structure

    1419
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP0A749
    SMRiP0A749
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A749

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni22 – 23Phosphoenolpyruvate bindingCombined sources2 Publications2
    Regioni120 – 124UDP-N-acetylglucosamine bindingCombined sources2 Publications5
    Regioni160 – 163UDP-N-acetylglucosamine bindingCombined sources2 Publications4

    Sequence similaritiesi

    Belongs to the EPSP synthase family. MurA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105CDF Bacteria
    COG0766 LUCA
    HOGENOMiHOG000075602
    InParanoidiP0A749
    KOiK00790
    OMAiCDPHRAT
    PhylomeDBiP0A749

    Family and domain databases

    CDDicd01555 UdpNAET, 1 hit
    Gene3Di3.65.10.10, 3 hits
    HAMAPiMF_00111 MurA, 1 hit
    InterProiView protein in InterPro
    IPR001986 Enolpyruvate_Tfrase_dom
    IPR036968 Enolpyruvate_Tfrase_sf
    IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
    IPR005750 UDP_GlcNAc_COvinyl_MurA
    PfamiView protein in Pfam
    PF00275 EPSP_synthase, 1 hit
    SUPFAMiSSF55205 SSF55205, 1 hit
    TIGRFAMsiTIGR01072 murA, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0A749-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MDKFRVQGPT KLQGEVTISG AKNAALPILF AALLAEEPVE IQNVPKLKDV
    60 70 80 90 100
    DTSMKLLSQL GAKVERNGSV HIDARDVNVF CAPYDLVKTM RASIWALGPL
    110 120 130 140 150
    VARFGQGQVS LPGGCTIGAR PVDLHISGLE QLGATIKLEE GYVKASVDGR
    160 170 180 190 200
    LKGAHIVMDK VSVGATVTIM CAATLAEGTT IIENAAREPE IVDTANFLIT
    210 220 230 240 250
    LGAKISGQGT DRIVIEGVER LGGGVYRVLP DRIETGTFLV AAAISRGKII
    260 270 280 290 300
    CRNAQPDTLD AVLAKLRDAG ADIEVGEDWI SLDMHGKRPK AVNVRTAPHP
    310 320 330 340 350
    AFPTDMQAQF TLLNLVAEGT GFITETVFEN RFMHVPELSR MGAHAEIESN
    360 370 380 390 400
    TVICHGVEKL SGAQVMATDL RASASLVLAG CIAEGTTVVD RIYHIDRGYE
    410
    RIEDKLRALG ANIERVKGE
    Length:419
    Mass (Da):44,818
    Last modified:June 7, 2005 - v1
    Checksum:i6B75A842255E53F7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M92358 Genomic DNA Translation: AAA24187.1
    U18997 Genomic DNA Translation: AAA57990.1
    U00096 Genomic DNA Translation: AAC76221.1
    AP009048 Genomic DNA Translation: BAE77233.1
    AB028039 Genomic DNA Translation: BAA78107.1
    PIRiA44917
    RefSeqiNP_417656.1, NC_000913.3
    WP_000357259.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC76221; AAC76221; b3189
    BAE77233; BAE77233; BAE77233
    GeneIDi947703
    KEGGiecj:JW3156
    eco:b3189
    PATRICifig|1411691.4.peg.3542

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M92358 Genomic DNA Translation: AAA24187.1
    U18997 Genomic DNA Translation: AAA57990.1
    U00096 Genomic DNA Translation: AAC76221.1
    AP009048 Genomic DNA Translation: BAE77233.1
    AB028039 Genomic DNA Translation: BAA78107.1
    PIRiA44917
    RefSeqiNP_417656.1, NC_000913.3
    WP_000357259.1, NZ_LN832404.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1A2NX-ray2.80A1-419[»]
    1UAEX-ray1.80A1-419[»]
    2Z2CX-ray2.05A/B/C/D1-419[»]
    3ISSX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L1-418[»]
    3KQJX-ray1.70A1-419[»]
    3KR6X-ray1.70A1-419[»]
    3SWDX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L1-418[»]
    ProteinModelPortaliP0A749
    SMRiP0A749
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262438, 644 interactors
    DIPiDIP-48060N
    IntActiP0A749, 25 interactors
    STRINGi316407.85675983

    Chemistry databases

    BindingDBiP0A749
    ChEMBLiCHEMBL1984
    DrugBankiDB00828 Fosfomycin

    Proteomic databases

    EPDiP0A749
    PaxDbiP0A749
    PRIDEiP0A749

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76221; AAC76221; b3189
    BAE77233; BAE77233; BAE77233
    GeneIDi947703
    KEGGiecj:JW3156
    eco:b3189
    PATRICifig|1411691.4.peg.3542

    Organism-specific databases

    EchoBASEiEB1333
    EcoGeneiEG11358 murA

    Phylogenomic databases

    eggNOGiENOG4105CDF Bacteria
    COG0766 LUCA
    HOGENOMiHOG000075602
    InParanoidiP0A749
    KOiK00790
    OMAiCDPHRAT
    PhylomeDBiP0A749

    Enzyme and pathway databases

    UniPathwayi
    UPA00219

    BioCyciEcoCyc:UDPNACETYLGLUCOSAMENOLPYRTRANS-MONOMER
    MetaCyc:UDPNACETYLGLUCOSAMENOLPYRTRANS-MONOMER
    BRENDAi2.5.1.7 2026

    Miscellaneous databases

    EvolutionaryTraceiP0A749
    PROiPR:P0A749

    Family and domain databases

    CDDicd01555 UdpNAET, 1 hit
    Gene3Di3.65.10.10, 3 hits
    HAMAPiMF_00111 MurA, 1 hit
    InterProiView protein in InterPro
    IPR001986 Enolpyruvate_Tfrase_dom
    IPR036968 Enolpyruvate_Tfrase_sf
    IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
    IPR005750 UDP_GlcNAc_COvinyl_MurA
    PfamiView protein in Pfam
    PF00275 EPSP_synthase, 1 hit
    SUPFAMiSSF55205 SSF55205, 1 hit
    TIGRFAMsiTIGR01072 murA, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMURA_ECOLI
    AccessioniPrimary (citable) accession number: P0A749
    Secondary accession number(s): P28909, Q2M923
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 7, 2005
    Last modified: September 12, 2018
    This is version 125 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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