UniProtKB - P0A746 (MSRB_ECOLI)
Protein
Peptide methionine sulfoxide reductase MsrB
Gene
msrB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- [thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(R)-S-oxide-[protein]1 PublicationEC:1.8.4.121 Publication
Cofactori
Zn2+1 PublicationNote: Binds 1 zinc ion per subunit. The zinc ion is important for the structural integrity of the protein.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 46 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 49 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 95 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 98 | ZincPROSITE-ProRule annotation | 1 | |
Active sitei | 118 | NucleophilePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- iron ion binding Source: EcoliWiki
- peptide-methionine (R)-S-oxide reductase activity Source: EcoliWiki
- zinc ion binding Source: EcoliWiki
GO - Biological processi
- protein repair Source: EcoCyc
- response to oxidative stress Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12394-MONOMER MetaCyc:EG12394-MONOMER |
BRENDAi | 1.8.4.12, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Peptide methionine sulfoxide reductase MsrB (EC:1.8.4.12)Alternative name(s): Peptide-methionine (R)-S-oxide reductase |
Gene namesi | Name:msrB Synonyms:yeaA Ordered Locus Names:b1778, JW1767 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 46 | C → D: Loss of activity; when associated with S-49; S-95 and S-98. 1 Publication | 1 | |
Mutagenesisi | 49 | C → S: Loss of activity; when associated with S-46; S-95 and S-98. 1 Publication | 1 | |
Mutagenesisi | 95 | C → S: Loss of activity; when associated with S-46; S-49 and S-98. 1 Publication | 1 | |
Mutagenesisi | 98 | C → S: Loss of activity; when associated with S-46; S-49 and S-95. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000140271 | 2 – 137 | Peptide methionine sulfoxide reductase MsrBAdd BLAST | 136 |
Proteomic databases
jPOSTi | P0A746 |
PaxDbi | P0A746 |
PRIDEi | P0A746 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260307, 54 interactors 851520, 1 interactor |
DIPi | DIP-48167N |
IntActi | P0A746, 4 interactors |
STRINGi | 511145.b1778 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 129 | MsrBPROSITE-ProRule annotationAdd BLAST | 123 |
Sequence similaritiesi
Belongs to the MsrB Met sulfoxide reductase family.Curated
Phylogenomic databases
eggNOGi | COG0229, Bacteria |
HOGENOMi | CLU_031040_8_5_6 |
InParanoidi | P0A746 |
PhylomeDBi | P0A746 |
Family and domain databases
HAMAPi | MF_01400, MsrB, 1 hit |
InterProi | View protein in InterPro IPR028427, Met_Sox_Rdtase IPR002579, Met_Sox_Rdtase_MsrB IPR011057, Mss4-like_sf |
PANTHERi | PTHR10173, PTHR10173, 1 hit |
Pfami | View protein in Pfam PF01641, SelR, 1 hit |
SUPFAMi | SSF51316, SSF51316, 1 hit |
TIGRFAMsi | TIGR00357, TIGR00357, 1 hit |
PROSITEi | View protein in PROSITE PS51790, MSRB, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A746-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANKPSAEEL KKNLSEMQFY VTQNHGTEPP FTGRLLHNKR DGVYHCLICD
60 70 80 90 100
APLFHSQTKY DSGCGWPSFY EPVSEESIRY IKDLSHGMQR IEIRCGNCDA
110 120 130
HLGHVFPDGP QPTGERYCVN SASLRFTDGE NGEEING
Sequence cautioni
The sequence X02662 differs from that shown. Reason: Frameshift.Curated
Mass spectrometryi
Molecular mass is 15319±1 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74848.1 AP009048 Genomic DNA Translation: BAA15575.2 X02662 Genomic DNA No translation available. |
PIRi | B64938 |
RefSeqi | NP_416292.1, NC_000913.3 WP_001284618.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74848; AAC74848; b1778 BAA15575; BAA15575; BAA15575 |
GeneIDi | 58462326 947188 |
KEGGi | ecj:JW1767 eco:b1778 |
PATRICi | fig|1411691.4.peg.476 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74848.1 AP009048 Genomic DNA Translation: BAA15575.2 X02662 Genomic DNA No translation available. |
PIRi | B64938 |
RefSeqi | NP_416292.1, NC_000913.3 WP_001284618.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6SYM | X-ray | 1.63 | A/B | 1-137 | [»] | |
SMRi | P0A746 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260307, 54 interactors 851520, 1 interactor |
DIPi | DIP-48167N |
IntActi | P0A746, 4 interactors |
STRINGi | 511145.b1778 |
Proteomic databases
jPOSTi | P0A746 |
PaxDbi | P0A746 |
PRIDEi | P0A746 |
Genome annotation databases
EnsemblBacteriai | AAC74848; AAC74848; b1778 BAA15575; BAA15575; BAA15575 |
GeneIDi | 58462326 947188 |
KEGGi | ecj:JW1767 eco:b1778 |
PATRICi | fig|1411691.4.peg.476 |
Organism-specific databases
EchoBASEi | EB2295 |
Phylogenomic databases
eggNOGi | COG0229, Bacteria |
HOGENOMi | CLU_031040_8_5_6 |
InParanoidi | P0A746 |
PhylomeDBi | P0A746 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12394-MONOMER MetaCyc:EG12394-MONOMER |
BRENDAi | 1.8.4.12, 2026 |
Miscellaneous databases
PROi | PR:P0A746 |
Family and domain databases
HAMAPi | MF_01400, MsrB, 1 hit |
InterProi | View protein in InterPro IPR028427, Met_Sox_Rdtase IPR002579, Met_Sox_Rdtase_MsrB IPR011057, Mss4-like_sf |
PANTHERi | PTHR10173, PTHR10173, 1 hit |
Pfami | View protein in Pfam PF01641, SelR, 1 hit |
SUPFAMi | SSF51316, SSF51316, 1 hit |
TIGRFAMsi | TIGR00357, TIGR00357, 1 hit |
PROSITEi | View protein in PROSITE PS51790, MSRB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MSRB_ECOLI | |
Accessioni | P0A746Primary (citable) accession number: P0A746 Secondary accession number(s): P39903, P76232, P76912 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | February 10, 2021 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families