UniProtKB - P0A744 (MSRA_ECOLI)
Protein
Peptide methionine sulfoxide reductase MsrA
Gene
msrA
Organism
Escherichia coli (strain K12)
Status
Functioni
Could have an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.1 Publication
Catalytic activityi
- [thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein]1 PublicationEC:1.8.4.111 Publication
- [thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-dithiol + L-methionine (S)-S-oxide1 PublicationEC:1.8.4.111 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 52 | Cysteine sulfenic acid (-SOH) intermediate1 Publication | 1 |
GO - Molecular functioni
- L-methionine-(S)-S-oxide reductase activity Source: EcoCyc
- oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor Source: EcoCyc
- peptide-methionine (S)-S-oxide reductase activity Source: EcoCyc
GO - Biological processi
- cellular protein modification process Source: UniProtKB-UniRule
- cellular response to oxidative stress Source: GO_Central
- protein repair Source: EcoCyc
- response to oxidative stress Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11433-MONOMER MetaCyc:EG11433-MONOMER |
BRENDAi | 1.8.4.11, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Peptide methionine sulfoxide reductase MsrA (EC:1.8.4.111 Publication)Short name: Protein-methionine-S-oxide reductase Alternative name(s): Peptide-methionine (S)-S-oxide reductase Short name: Peptide Met(O) reductase |
Gene namesi | Name:msrA Synonyms:pms Ordered Locus Names:b4219, JW4178 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | C → S: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 87 | C → S: No effect. 1 Publication | 1 | |
Mutagenesisi | 199 | C → S: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 207 | C → S: Decrease in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000138546 | 2 – 212 | Peptide methionine sulfoxide reductase MsrAAdd BLAST | 211 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 52 ↔ 199 | Redox-active; alternate1 Publication | ||
Disulfide bondi | 199 ↔ 207 | Redox-active; alternate1 Publication |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P0A744 |
PaxDbi | P0A744 |
PRIDEi | P0A744 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259307, 41 interactors 853026, 1 interactor |
IntActi | P0A744, 4 interactors |
STRINGi | 511145.b4219 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A744 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A744 |
Family & Domainsi
Sequence similaritiesi
Belongs to the MsrA Met sulfoxide reductase family.Curated
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | COG0225, Bacteria |
HOGENOMi | CLU_031040_10_3_6 |
InParanoidi | P0A744 |
PhylomeDBi | P0A744 |
Family and domain databases
Gene3Di | 3.30.1060.10, 1 hit |
HAMAPi | MF_01401, MsrA, 1 hit |
InterProi | View protein in InterPro IPR002569, Met_Sox_Rdtase_MsrA IPR036509, Met_Sox_Rdtase_MsrA_sf |
Pfami | View protein in Pfam PF01625, PMSR, 1 hit |
SUPFAMi | SSF55068, SSF55068, 1 hit |
TIGRFAMsi | TIGR00401, msrA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A744-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM
60 70 80 90 100
GCFWGVERLF WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV
110 120 130 140 150
YDPSVISYEQ LLQVFWENHD PAQGMRQGND HGTQYRSAIY PLTPEQDAAA
160 170 180 190 200
RASLERFQAA MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG
210
IGGIGVCLPP EA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M89992 Genomic DNA Translation: AAA24399.1 U14003 Genomic DNA Translation: AAA97115.1 U00096 Genomic DNA Translation: AAC77176.1 AP009048 Genomic DNA Translation: BAE78220.1 |
PIRi | S56444 |
RefSeqi | NP_418640.1, NC_000913.3 WP_001295196.1, NZ_STEB01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAC77176; AAC77176; b4219 BAE78220; BAE78220; BAE78220 |
GeneIDi | 58463647 948734 |
KEGGi | ecj:JW4178 eco:b4219 |
PATRICi | fig|1411691.4.peg.2482 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M89992 Genomic DNA Translation: AAA24399.1 U14003 Genomic DNA Translation: AAA97115.1 U00096 Genomic DNA Translation: AAC77176.1 AP009048 Genomic DNA Translation: BAE78220.1 |
PIRi | S56444 |
RefSeqi | NP_418640.1, NC_000913.3 WP_001295196.1, NZ_STEB01000013.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FF3 | X-ray | 1.90 | A/B/C | 2-212 | [»] | |
2GT3 | NMR | - | A | 1-212 | [»] | |
2IEM | NMR | - | A | 2-212 | [»] | |
SMRi | P0A744 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259307, 41 interactors 853026, 1 interactor |
IntActi | P0A744, 4 interactors |
STRINGi | 511145.b4219 |
Proteomic databases
jPOSTi | P0A744 |
PaxDbi | P0A744 |
PRIDEi | P0A744 |
Genome annotation databases
EnsemblBacteriai | AAC77176; AAC77176; b4219 BAE78220; BAE78220; BAE78220 |
GeneIDi | 58463647 948734 |
KEGGi | ecj:JW4178 eco:b4219 |
PATRICi | fig|1411691.4.peg.2482 |
Organism-specific databases
EchoBASEi | EB1403 |
Phylogenomic databases
eggNOGi | COG0225, Bacteria |
HOGENOMi | CLU_031040_10_3_6 |
InParanoidi | P0A744 |
PhylomeDBi | P0A744 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11433-MONOMER MetaCyc:EG11433-MONOMER |
BRENDAi | 1.8.4.11, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A744 |
PROi | PR:P0A744 |
Family and domain databases
Gene3Di | 3.30.1060.10, 1 hit |
HAMAPi | MF_01401, MsrA, 1 hit |
InterProi | View protein in InterPro IPR002569, Met_Sox_Rdtase_MsrA IPR036509, Met_Sox_Rdtase_MsrA_sf |
Pfami | View protein in Pfam PF01625, PMSR, 1 hit |
SUPFAMi | SSF55068, SSF55068, 1 hit |
TIGRFAMsi | TIGR00401, msrA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MSRA_ECOLI | |
Accessioni | P0A744Primary (citable) accession number: P0A744 Secondary accession number(s): P27110, Q2M686 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families