UniProtKB - P0A738 (MOAC_ECOLI)
Protein
Cyclic pyranopterin monophosphate synthase
Gene
moaC
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).UniRule annotation1 Publication
Catalytic activityi
- (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphateUniRule annotation1 PublicationEC:4.6.1.17UniRule annotation1 Publication
Kineticsi
kcat is 0.56 min(-1).1 Publication
- KM=0.21 µM for (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate1 Publication
: molybdopterin biosynthesis Pathwayi
This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 128 | UniRule annotation | 1 |
GO - Molecular functioni
- cyclic pyranopterin monophosphate synthase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
GO - Biological processi
- Mo-molybdopterin cofactor biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Biological process | Molybdenum cofactor biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11666-MONOMER MetaCyc:EG11666-MONOMER |
UniPathwayi | UPA00344 |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclic pyranopterin monophosphate synthaseUniRule annotationCurated (EC:4.6.1.17UniRule annotation1 Publication)Alternative name(s): Molybdenum cofactor biosynthesis protein CUniRule annotation |
Gene namesi | Name:moaC Synonyms:chlA3 Ordered Locus Names:b0783, JW0766 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 17 | D → A: Loss of activity by 70%. 1 Publication | 1 | |
Mutagenesisi | 21 | K → A: Loss of activity by 85%. 1 Publication | 1 | |
Mutagenesisi | 26 | R → A: Loss of activity by 80%. 1 Publication | 1 | |
Mutagenesisi | 51 | K → A: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 52 | G → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 67 | K → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 76 | C → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 77 | H → A: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 112 | E → A: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 114 | E → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 128 | D → A: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 131 | K → A: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 147 | K → A: Loss of activity by 90%. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000097798 | 2 – 161 | Cyclic pyranopterin monophosphate synthaseAdd BLAST | 160 |
Proteomic databases
jPOSTi | P0A738 |
PaxDbi | P0A738 |
PRIDEi | P0A738 |
Expressioni
Inductioni
By anaerobiosis, repressed by the molybdenum cofactor.
Interactioni
Subunit structurei
Homohexamer; trimer of dimers.
UniRule annotation1 PublicationBinary interactionsi
P0A738
With | #Exp. | IntAct |
---|---|---|
rplA [P0A7L0] | 2 | EBI-554314,EBI-543771 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4262176, 46 interactors |
DIPi | DIP-10230N |
IntActi | P0A738, 20 interactors |
STRINGi | 511145.b0783 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A738 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A738 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 75 – 77 | Substrate bindingUniRule annotation1 Publication | 3 | |
Regioni | 113 – 114 | Substrate bindingUniRule annotation1 Publication | 2 |
Sequence similaritiesi
Belongs to the MoaC family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0315, Bacteria |
HOGENOMi | CLU_074693_1_1_6 |
InParanoidi | P0A738 |
PhylomeDBi | P0A738 |
Family and domain databases
CDDi | cd01420, MoaC_PE, 1 hit |
Gene3Di | 3.30.70.640, 1 hit |
HAMAPi | MF_01224_B, MoaC_B, 1 hit |
InterProi | View protein in InterPro IPR023045, Mo_CF_biosynth-C IPR036522, MoaC_sf IPR002820, Mopterin_CF_biosynth-C_dom |
Pfami | View protein in Pfam PF01967, MoaC, 1 hit |
SUPFAMi | SSF55040, SSF55040, 1 hit |
TIGRFAMsi | TIGR00581, moaC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A738-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQLTHINAA GEAHMVDVSA KAETVREARA EAFVTMRSET LAMIIDGRHH
60 70 80 90 100
KGDVFATARI AGIQAAKRTW DLIPLCHPLM LSKVEVNLQA EPEHNRVRIE
110 120 130 140 150
TLCRLTGKTG VEMEALTAAS VAALTIYDMC KAVQKDMVIG PVRLLAKSGG
160
KSGDFKVEAD D
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 17 | D → N in CAA49863 (PubMed:8361352).Curated | 1 | |
Sequence conflicti | 92 – 97 | PEHNRV → RAQSG in CAA49863 (PubMed:8361352).Curated | 6 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X70420 Genomic DNA Translation: CAA49863.1 U00096 Genomic DNA Translation: AAC73870.1 AP009048 Genomic DNA Translation: BAA35441.1 |
PIRi | G64814 |
RefSeqi | NP_415304.1, NC_000913.3 WP_000080885.1, NZ_STEB01000028.1 |
Genome annotation databases
EnsemblBacteriai | AAC73870; AAC73870; b0783 BAA35441; BAA35441; BAA35441 |
GeneIDi | 52076089 945397 |
KEGGi | ecj:JW0766 eco:b0783 |
PATRICi | fig|1411691.4.peg.1495 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X70420 Genomic DNA Translation: CAA49863.1 U00096 Genomic DNA Translation: AAC73870.1 AP009048 Genomic DNA Translation: BAA35441.1 |
PIRi | G64814 |
RefSeqi | NP_415304.1, NC_000913.3 WP_000080885.1, NZ_STEB01000028.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EKR | X-ray | 2.00 | A | 1-161 | [»] | |
1EKS | X-ray | 2.50 | A | 1-161 | [»] | |
4PYA | X-ray | 1.79 | A | 3-161 | [»] | |
4PYD | X-ray | 3.19 | A/B/C/D/E/F | 1-161 | [»] | |
SMRi | P0A738 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262176, 46 interactors |
DIPi | DIP-10230N |
IntActi | P0A738, 20 interactors |
STRINGi | 511145.b0783 |
Proteomic databases
jPOSTi | P0A738 |
PaxDbi | P0A738 |
PRIDEi | P0A738 |
Genome annotation databases
EnsemblBacteriai | AAC73870; AAC73870; b0783 BAA35441; BAA35441; BAA35441 |
GeneIDi | 52076089 945397 |
KEGGi | ecj:JW0766 eco:b0783 |
PATRICi | fig|1411691.4.peg.1495 |
Organism-specific databases
EchoBASEi | EB1617 |
Phylogenomic databases
eggNOGi | COG0315, Bacteria |
HOGENOMi | CLU_074693_1_1_6 |
InParanoidi | P0A738 |
PhylomeDBi | P0A738 |
Enzyme and pathway databases
UniPathwayi | UPA00344 |
BioCyci | EcoCyc:EG11666-MONOMER MetaCyc:EG11666-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A738 |
PROi | PR:P0A738 |
Family and domain databases
CDDi | cd01420, MoaC_PE, 1 hit |
Gene3Di | 3.30.70.640, 1 hit |
HAMAPi | MF_01224_B, MoaC_B, 1 hit |
InterProi | View protein in InterPro IPR023045, Mo_CF_biosynth-C IPR036522, MoaC_sf IPR002820, Mopterin_CF_biosynth-C_dom |
Pfami | View protein in Pfam PF01967, MoaC, 1 hit |
SUPFAMi | SSF55040, SSF55040, 1 hit |
TIGRFAMsi | TIGR00581, moaC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MOAC_ECOLI | |
Accessioni | P0A738Primary (citable) accession number: P0A738 Secondary accession number(s): P30747, P77530 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families