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UniProtKB - P0A731 (MGSA_ECOLI)
Protein
Methylglyoxal synthase
Gene
mgsA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the formation of methylglyoxal from dihydroxyacetone phosphate.
UniRule annotation3 PublicationsCatalytic activityi
- EC:4.2.3.3UniRule annotation3 Publications
Activity regulationi
Inhibited by inorganic phosphate ions (PubMed:9665712, PubMed:15049687). Competitively inhibited by phosphoglycolate (PubMed:15049687). Competitively inhibited by phosphoglycolohydroxamate (PubMed:11389594).3 Publications
Kineticsi
kcat is 220 sec(-1) with dihydroxyacetone phosphate as substrate.1 Publication
- KM=0.2 mM for dihydroxyacetone phosphate1 Publication
pH dependencei
Optimum pH is 7.5.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 19 | SubstrateUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 23 | SubstrateUniRule annotationCombined sources3 Publications | 1 | |
Active sitei | 71 | Proton donor/acceptorUniRule annotation4 Publications | 1 | |
Binding sitei | 98 | SubstrateUniRule annotationCombined sources2 Publications | 1 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
- methylglyoxal synthase activity Source: EcoCyc
GO - Biological processi
- methylglyoxal biosynthetic process Source: EcoCyc
- protein hexamerization Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Enzyme and pathway databases
BioCyci | EcoCyc:METHGLYSYN-MONOMER |
BRENDAi | 4.2.3.3, 2026 |
SABIO-RKi | P0A731 |
Names & Taxonomyi
Protein namesi | Recommended name: Methylglyoxal synthaseUniRule annotation (EC:4.2.3.3UniRule annotation3 Publications)Short name: MGSUniRule annotation |
Gene namesi | Name:mgsAUniRule annotation Synonyms:yccG Ordered Locus Names:b0963, JW5129 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 20 | D → E: Strongly decreased catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 20 | D → N: Causes inhibition by substrate leading to loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 71 | D → E or N: Strongly decreased catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 91 | D → E: Strongly decreased catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 91 | D → N: Causes inhibition by substrate leading to loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 98 | H → N or Q: Strongly decreased catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 101 | D → E or N: Strongly decreased catalytic efficiency. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000178625 | 1 – 152 | Methylglyoxal synthaseAdd BLAST | 152 |
Proteomic databases
jPOSTi | P0A731 |
PaxDbi | P0A731 |
PRIDEi | P0A731 |
Interactioni
Subunit structurei
Homohexamer.
4 PublicationsGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260938, 11 interactors 849948, 1 interactor |
DIPi | DIP-48252N |
STRINGi | 511145.b0963 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0A731 |
SMRi | P0A731 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A731 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 152 | MGS-likeUniRule annotationAdd BLAST | 147 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 45 – 48 | Substrate bindingUniRule annotationCombined sources3 Publications | 4 | |
Regioni | 65 – 66 | Substrate bindingUniRule annotationCombined sources3 Publications | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1803, Bacteria |
HOGENOMi | CLU_120420_0_1_6 |
InParanoidi | P0A731 |
OMAi | RACDVHN |
PhylomeDBi | P0A731 |
Family and domain databases
CDDi | cd01422, MGS, 1 hit |
Gene3Di | 3.40.50.1380, 1 hit |
HAMAPi | MF_00549, Methylglyoxal_synth, 1 hit |
InterProi | View protein in InterPro IPR004363, Methylgl_synth IPR018148, Methylglyoxal_synth_AS IPR011607, MGS-like_dom IPR036914, MGS-like_dom_sf |
PANTHERi | PTHR30492, PTHR30492, 1 hit |
Pfami | View protein in Pfam PF02142, MGS, 1 hit |
PIRSFi | PIRSF006614, Methylglyox_syn, 1 hit |
SMARTi | View protein in SMART SM00851, MGS, 1 hit |
SUPFAMi | SSF52335, SSF52335, 1 hit |
TIGRFAMsi | TIGR00160, MGSA, 1 hit |
PROSITEi | View protein in PROSITE PS01335, METHYLGLYOXAL_SYNTH, 1 hit PS51855, MGS, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A731-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELTTRTLPA RKHIALVAHD HCKQMLMSWV ERHQPLLEQH VLYATGTTGN
60 70 80 90 100
LISRATGMNV NAMLSGPMGG DQQVGALISE GKIDVLIFFW DPLNAVPHDP
110 120 130 140 150
DVKALLRLAT VWNIPVATNV ATADFIIQSP HFNDAVDILI PDYQRYLADR
LK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 2 | ME → WK in J04726 (PubMed:2542273).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04726 Genomic DNA No translation available. Y11249 Genomic DNA Translation: CAA72119.1 U00096 Genomic DNA Translation: AAC74049.2 AP009048 Genomic DNA Translation: BAA35728.2 |
PIRi | B64837 |
RefSeqi | NP_415483.2, NC_000913.3 WP_000424181.1, NZ_STEB01000006.1 |
Genome annotation databases
EnsemblBacteriai | AAC74049; AAC74049; b0963 BAA35728; BAA35728; BAA35728 |
GeneIDi | 66670761 945574 |
KEGGi | ecj:JW5129 eco:b0963 |
PATRICi | fig|1411691.4.peg.1311 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04726 Genomic DNA No translation available. Y11249 Genomic DNA Translation: CAA72119.1 U00096 Genomic DNA Translation: AAC74049.2 AP009048 Genomic DNA Translation: BAA35728.2 |
PIRi | B64837 |
RefSeqi | NP_415483.2, NC_000913.3 WP_000424181.1, NZ_STEB01000006.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1B93 | X-ray | 1.90 | A/B/C | 1-152 | [»] | |
1EGH | X-ray | 2.00 | A/B/C/D/E/F | 1-152 | [»] | |
1IK4 | X-ray | 2.00 | A/B/C/D/E/F | 1-152 | [»] | |
1S89 | X-ray | 2.22 | A/B/C/D/E/F | 1-152 | [»] | |
1S8A | X-ray | 2.20 | A/B/C/D/E/F | 1-152 | [»] | |
AlphaFoldDBi | P0A731 | |||||
SMRi | P0A731 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260938, 11 interactors 849948, 1 interactor |
DIPi | DIP-48252N |
STRINGi | 511145.b0963 |
Proteomic databases
jPOSTi | P0A731 |
PaxDbi | P0A731 |
PRIDEi | P0A731 |
Genome annotation databases
EnsemblBacteriai | AAC74049; AAC74049; b0963 BAA35728; BAA35728; BAA35728 |
GeneIDi | 66670761 945574 |
KEGGi | ecj:JW5129 eco:b0963 |
PATRICi | fig|1411691.4.peg.1311 |
Organism-specific databases
EchoBASEi | EB2213 |
Phylogenomic databases
eggNOGi | COG1803, Bacteria |
HOGENOMi | CLU_120420_0_1_6 |
InParanoidi | P0A731 |
OMAi | RACDVHN |
PhylomeDBi | P0A731 |
Enzyme and pathway databases
BioCyci | EcoCyc:METHGLYSYN-MONOMER |
BRENDAi | 4.2.3.3, 2026 |
SABIO-RKi | P0A731 |
Miscellaneous databases
EvolutionaryTracei | P0A731 |
PROi | PR:P0A731 |
Family and domain databases
CDDi | cd01422, MGS, 1 hit |
Gene3Di | 3.40.50.1380, 1 hit |
HAMAPi | MF_00549, Methylglyoxal_synth, 1 hit |
InterProi | View protein in InterPro IPR004363, Methylgl_synth IPR018148, Methylglyoxal_synth_AS IPR011607, MGS-like_dom IPR036914, MGS-like_dom_sf |
PANTHERi | PTHR30492, PTHR30492, 1 hit |
Pfami | View protein in Pfam PF02142, MGS, 1 hit |
PIRSFi | PIRSF006614, Methylglyox_syn, 1 hit |
SMARTi | View protein in SMART SM00851, MGS, 1 hit |
SUPFAMi | SSF52335, SSF52335, 1 hit |
TIGRFAMsi | TIGR00160, MGSA, 1 hit |
PROSITEi | View protein in PROSITE PS01335, METHYLGLYOXAL_SYNTH, 1 hit PS51855, MGS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MGSA_ECOLI | |
Accessioni | P0A731Primary (citable) accession number: P0A731 Secondary accession number(s): P37066 Q9R7Q3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | May 25, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families