UniProtKB - P0A722 (LPXA_ECOLI)
Protein
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Gene
lpxA
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.
Catalytic activityi
- (3R)-hydroxytetradecanoyl-[ACP] + UDP-N-acetyl-α-D-glucosamine = holo-[ACP] + UDP-3-O-[(3R)-3-hydroxytetradecanoyl]-N-acetyl-α-D-glucosamineUniRule annotationEC:2.3.1.129UniRule annotation
: lipid IV(A) biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotationProteins known to be involved in the 6 steps of the subpathway in this organism are:
- Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA), Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
- UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC), UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
- UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD), UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD)
- UDP-2,3-diacylglucosamine hydrolase (lpxH), UDP-2,3-diacylglucosamine hydrolase (lpxH)
- Lipid-A-disaccharide synthase (lpxB), Lipid-A-disaccharide synthase (lpxB)
- Tetraacyldisaccharide 4'-kinase (lpxK), Tetraacyldisaccharide 4'-kinase (lpxK)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 125 | Substrate | 1 | |
Binding sitei | 144 | Substrate | 1 | |
Binding sitei | 161 | Substrate | 1 |
GO - Molecular functioni
- acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Source: EcoliWiki
- identical protein binding Source: EcoCyc
GO - Biological processi
- lipid A biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BioCyci | EcoCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER MetaCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER |
BRENDAi | 2.3.1.129, 2026 |
UniPathwayi | UPA00359;UER00477 |
Chemistry databases
SwissLipidsi | SLP:000001883 |
Names & Taxonomyi
Protein namesi | Recommended name: Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseUniRule annotation (EC:2.3.1.129UniRule annotation)Short name: UDP-N-acetylglucosamine acyltransferaseUniRule annotation |
Gene namesi | Name:lpxAUniRule annotation Ordered Locus Names:b0181, JW0176 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000188046 | 1 – 262 | Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseAdd BLAST | 262 |
Proteomic databases
jPOSTi | P0A722 |
PaxDbi | P0A722 |
PRIDEi | P0A722 |
Interactioni
Subunit structurei
Homotrimer.
UniRule annotation3 PublicationsGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261091, 390 interactors |
DIPi | DIP-48043N |
IntActi | P0A722, 36 interactors |
STRINGi | 511145.b0181 |
Chemistry databases
BindingDBi | P0A722 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A722 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A722 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 73 – 76 | Substrate binding | 4 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG1043, Bacteria |
HOGENOMi | CLU_061249_0_0_6 |
InParanoidi | P0A722 |
PhylomeDBi | P0A722 |
Family and domain databases
CDDi | cd03351, LbH_UDP-GlcNAc_AT, 1 hit |
Gene3Di | 1.20.1180.10, 1 hit |
HAMAPi | MF_00387, LpxA, 1 hit |
InterProi | View protein in InterPro IPR029098, Acetyltransf_C IPR037157, Acetyltransf_C_sf IPR001451, Hexapep IPR018357, Hexapep_transf_CS IPR010137, Lipid_A_LpxA IPR011004, Trimer_LpxA-like_sf |
PANTHERi | PTHR43480, PTHR43480, 1 hit |
Pfami | View protein in Pfam PF13720, Acetyltransf_11, 1 hit PF00132, Hexapep, 2 hits |
PIRSFi | PIRSF000456, UDP-GlcNAc_acltr, 1 hit |
SUPFAMi | SSF51161, SSF51161, 1 hit |
TIGRFAMsi | TIGR01852, lipid_A_lpxA, 1 hit |
PROSITEi | View protein in PROSITE PS00101, HEXAPEP_TRANSFERASES, 2 hits |
i Sequence
Sequence statusi: Complete.
P0A722-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIDKSAFVHP TAIVEEGASI GANAHIGPFC IVGPHVEIGE GTVLKSHVVV
60 70 80 90 100
NGHTKIGRDN EIYQFASIGE VNQDLKYAGE PTRVEIGDRN RIRESVTIHR
110 120 130 140 150
GTVQGGGLTK VGSDNLLMIN AHIAHDCTVG NRCILANNAT LAGHVSVDDF
160 170 180 190 200
AIIGGMTAVH QFCIIGAHVM VGGCSGVAQD VPPYVIAQGN HATPFGVNIE
210 220 230 240 250
GLKRRGFSRE AITAIRNAYK LIYRSGKTLD EVKPEIAELA ETYPEVKAFT
260
DFFARSTRGL IR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 64 – 65 | QF → SV in AAC36918 (PubMed:3277952).Curated | 2 | |
Sequence conflicti | 125 | H → D in AAC36918 (PubMed:3277952).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 189 | G → S in strain: SM101; temperature-sensitive. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19334 Genomic DNA Translation: AAC36918.1 U70214 Genomic DNA Translation: AAB08610.1 U00096 Genomic DNA Translation: AAC73292.1 AP009048 Genomic DNA Translation: BAA77856.2 |
PIRi | E64742, XUECDP |
RefSeqi | NP_414723.1, NC_000913.3 WP_000565966.1, NZ_STEB01000032.1 |
Genome annotation databases
EnsemblBacteriai | AAC73292; AAC73292; b0181 BAA77856; BAA77856; BAA77856 |
GeneIDi | 58388405 944849 |
KEGGi | ecj:JW0176 eco:b0181 |
PATRICi | fig|1411691.4.peg.2098 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19334 Genomic DNA Translation: AAC36918.1 U70214 Genomic DNA Translation: AAB08610.1 U00096 Genomic DNA Translation: AAC73292.1 AP009048 Genomic DNA Translation: BAA77856.2 |
PIRi | E64742, XUECDP |
RefSeqi | NP_414723.1, NC_000913.3 WP_000565966.1, NZ_STEB01000032.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1LXA | X-ray | 2.60 | A | 1-262 | [»] | |
2AQ9 | X-ray | 1.80 | A | 1-262 | [»] | |
2JF2 | X-ray | 1.80 | A | 1-262 | [»] | |
2JF3 | X-ray | 3.00 | A | 1-262 | [»] | |
2QIA | X-ray | 1.74 | A | 1-262 | [»] | |
2QIV | X-ray | 1.85 | X | 1-262 | [»] | |
4J09 | X-ray | 1.90 | A | 1-262 | [»] | |
6HY2 | X-ray | 1.60 | X | 1-262 | [»] | |
6P9P | X-ray | 2.00 | A | 1-262 | [»] | |
6P9Q | X-ray | 1.70 | A | 1-262 | [»] | |
6P9R | X-ray | 1.75 | A | 1-262 | [»] | |
6P9S | X-ray | 1.70 | A | 1-262 | [»] | |
6P9T | X-ray | 1.75 | A | 1-262 | [»] | |
SMRi | P0A722 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261091, 390 interactors |
DIPi | DIP-48043N |
IntActi | P0A722, 36 interactors |
STRINGi | 511145.b0181 |
Chemistry databases
BindingDBi | P0A722 |
SwissLipidsi | SLP:000001883 |
Proteomic databases
jPOSTi | P0A722 |
PaxDbi | P0A722 |
PRIDEi | P0A722 |
Genome annotation databases
EnsemblBacteriai | AAC73292; AAC73292; b0181 BAA77856; BAA77856; BAA77856 |
GeneIDi | 58388405 944849 |
KEGGi | ecj:JW0176 eco:b0181 |
PATRICi | fig|1411691.4.peg.2098 |
Organism-specific databases
EchoBASEi | EB0540 |
Phylogenomic databases
eggNOGi | COG1043, Bacteria |
HOGENOMi | CLU_061249_0_0_6 |
InParanoidi | P0A722 |
PhylomeDBi | P0A722 |
Enzyme and pathway databases
UniPathwayi | UPA00359;UER00477 |
BioCyci | EcoCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER MetaCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER |
BRENDAi | 2.3.1.129, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0A722 |
PROi | PR:P0A722 |
Family and domain databases
CDDi | cd03351, LbH_UDP-GlcNAc_AT, 1 hit |
Gene3Di | 1.20.1180.10, 1 hit |
HAMAPi | MF_00387, LpxA, 1 hit |
InterProi | View protein in InterPro IPR029098, Acetyltransf_C IPR037157, Acetyltransf_C_sf IPR001451, Hexapep IPR018357, Hexapep_transf_CS IPR010137, Lipid_A_LpxA IPR011004, Trimer_LpxA-like_sf |
PANTHERi | PTHR43480, PTHR43480, 1 hit |
Pfami | View protein in Pfam PF13720, Acetyltransf_11, 1 hit PF00132, Hexapep, 2 hits |
PIRSFi | PIRSF000456, UDP-GlcNAc_acltr, 1 hit |
SUPFAMi | SSF51161, SSF51161, 1 hit |
TIGRFAMsi | TIGR01852, lipid_A_lpxA, 1 hit |
PROSITEi | View protein in PROSITE PS00101, HEXAPEP_TRANSFERASES, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LPXA_ECOLI | |
Accessioni | P0A722Primary (citable) accession number: P0A722 Secondary accession number(s): P10440, P78243 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | April 7, 2021 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families