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Protein

Chaperone protein HscA

Gene

hscA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU.

GO - Molecular functioni

  • ADP binding Source: EcoCyc
  • ATPase activity Source: EcoCyc
  • ATPase activity, coupled Source: GO_Central
  • ATP binding Source: EcoCyc
  • heat shock protein binding Source: GO_Central
  • misfolded protein binding Source: GO_Central
  • protein binding involved in protein folding Source: GO_Central
  • unfolded protein binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12130-MONOMER
MetaCyc:EG12130-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein HscA
Alternative name(s):
Hsc66
Gene namesi
Name:hscA
Synonyms:hsc
Ordered Locus Names:b2526, JW2510
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12130 hscA

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000786251 – 616Chaperone protein HscAAdd BLAST616

Proteomic databases

EPDiP0A6Z1
PaxDbiP0A6Z1
PRIDEiP0A6Z1

Expressioni

Inductioni

By cold shock.1 Publication

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4262906, 75 interactors
DIPiDIP-47348N
IntActiP0A6Z1, 28 interactors
STRINGi316385.ECDH10B_2693

Structurei

Secondary structure

1616
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0A6Z1
SMRiP0A6Z1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6Z1

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiENOG4105C9I Bacteria
COG0443 LUCA
HOGENOMiHOG000228136
InParanoidiP0A6Z1
KOiK04044
PhylomeDBiP0A6Z1

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
HAMAPiMF_00679 HscA, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
IPR010236 ISC_FeS_clus_asmbl_HscA
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
TIGRFAMsiTIGR01991 HscA, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequencei

Sequence statusi: Complete.

P0A6Z1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALLQISEPG LSAAPHQRRL AAGIDLGTTN SLVATVRSGQ AETLADHEGR
60 70 80 90 100
HLLPSVVHYQ QQGHSVGYDA RTNAALDTAN TISSVKRLMG RSLADIQQRY
110 120 130 140 150
PHLPYQFQAS ENGLPMIETA AGLLNPVRVS ADILKALAAR ATEALAGELD
160 170 180 190 200
GVVITVPAYF DDAQRQGTKD AARLAGLHVL RLLNEPTAAA IAYGLDSGQE
210 220 230 240 250
GVIAVYDLGG GTFDISILRL SRGVFEVLAT GGDSALGGDD FDHLLADYIR
260 270 280 290 300
EQAGIPDRSD NRVQRELLDA AIAAKIALSD ADSVTVNVAG WQGEISREQF
310 320 330 340 350
NELIAPLVKR TLLACRRALK DAGVEADEVL EVVMVGGSTR VPLVRERVGE
360 370 380 390 400
FFGRPPLTSI DPDKVVAIGA AIQADILVGN KPDSEMLLLD VIPLSLGLET
410 420 430 440 450
MGGLVEKVIP RNTTIPVARA QDFTTFKDGQ TAMSIHVMQG ERELVQDCRS
460 470 480 490 500
LARFALRGIP ALPAGGAHIR VTFQVDADGL LSVTAMEKST GVEASIQVKP
510 520 530 540 550
SYGLTDSEIA SMIKDSMSYA EQDVKARMLA EQKVEAARVL ESLHGALAAD
560 570 580 590 600
AALLSAAERQ VIDDAAAHLS EVAQGDDVDA IEQAIKNVDK QTQDFAARRM
610
DQSVRRALKG HSVDEV
Length:616
Mass (Da):65,652
Last modified:June 7, 2005 - v1
Checksum:i9C80E6EF1BFDAB48
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti456 – 465LRGIPALPAG → CVVFRRYRLA in AAA18300 (PubMed:8300516).Curated10
Sequence conflicti516S → T in AAA18300 (PubMed:8300516).Curated1
Sequence conflicti557Missing in AAA18300 (PubMed:8300516).Curated1
Sequence conflicti583Q → K in AAD13473 (PubMed:8134349).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti216S → F in hsca1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01827 Unassigned DNA Translation: AAA18300.1
U05338 Genomic DNA Translation: AAD13473.1
U00096 Genomic DNA Translation: AAC75579.1
AP009048 Genomic DNA Translation: BAA16420.1
PIRiE65029
RefSeqiNP_417021.1, NC_000913.3
WP_001196613.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75579; AAC75579; b2526
BAA16420; BAA16420; BAA16420
GeneIDi944885
KEGGiecj:JW2510
eco:b2526
PATRICifig|1411691.4.peg.4208

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01827 Unassigned DNA Translation: AAA18300.1
U05338 Genomic DNA Translation: AAD13473.1
U00096 Genomic DNA Translation: AAC75579.1
AP009048 Genomic DNA Translation: BAA16420.1
PIRiE65029
RefSeqiNP_417021.1, NC_000913.3
WP_001196613.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U00X-ray1.95A389-615[»]
ProteinModelPortaliP0A6Z1
SMRiP0A6Z1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262906, 75 interactors
DIPiDIP-47348N
IntActiP0A6Z1, 28 interactors
STRINGi316385.ECDH10B_2693

Proteomic databases

EPDiP0A6Z1
PaxDbiP0A6Z1
PRIDEiP0A6Z1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75579; AAC75579; b2526
BAA16420; BAA16420; BAA16420
GeneIDi944885
KEGGiecj:JW2510
eco:b2526
PATRICifig|1411691.4.peg.4208

Organism-specific databases

EchoBASEiEB2051
EcoGeneiEG12130 hscA

Phylogenomic databases

eggNOGiENOG4105C9I Bacteria
COG0443 LUCA
HOGENOMiHOG000228136
InParanoidiP0A6Z1
KOiK04044
PhylomeDBiP0A6Z1

Enzyme and pathway databases

BioCyciEcoCyc:EG12130-MONOMER
MetaCyc:EG12130-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0A6Z1
PROiPR:P0A6Z1

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
HAMAPiMF_00679 HscA, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
IPR010236 ISC_FeS_clus_asmbl_HscA
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
TIGRFAMsiTIGR01991 HscA, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHSCA_ECOLI
AccessioniPrimary (citable) accession number: P0A6Z1
Secondary accession number(s): P36541, P76990, P77497
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 7, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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