UniProtKB - P0A6Y8 (DNAK_ECOLI)
Protein
Chaperone protein DnaK
Gene
dnaK
Organism
Escherichia coli (strain K12)
Status
Functioni
Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.
GO - Molecular functioni
- ADP binding Source: EcoCyc
- ATPase activity Source: CAFA
- ATP binding Source: CAFA
- chaperone binding Source: CAFA
- protein folding chaperone Source: EcoCyc
- sigma factor antagonist activity Source: EcoCyc
- unfolded protein binding Source: CAFA
- zinc ion binding Source: EcoliWiki
GO - Biological processi
- cellular response to unfolded protein Source: EcoCyc
- chaperone cofactor-dependent protein refolding Source: CAFA
- DNA replication Source: UniProtKB-KW
- protein-containing complex assembly Source: CAFA
- protein-containing complex disassembly Source: EcoCyc
- response to heat Source: EcoCyc
Keywordsi
Molecular function | Chaperone |
Biological process | DNA replication, Stress response |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10241-MONOMER MetaCyc:EG10241-MONOMER |
Protein family/group databases
TCDBi | 1.A.33.1.2, the cation channel-forming heat shock protein-70 (hsp70) family |
Names & Taxonomyi
Protein namesi | Recommended name: Chaperone protein DnaKAlternative name(s): HSP70 Heat shock 70 kDa protein Heat shock protein 70 |
Gene namesi | Name:dnaK Synonyms:groP, grpF, seg Ordered Locus Names:b0014, JW0013 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Plasma membrane
- Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication
Cytosol
- cytosol Source: EcoCyc
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB
- inclusion body Source: CACAO
- membrane Source: UniProtKB
- protein-containing complex Source: CAFA
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, Cytoplasm, MembranePathology & Biotechi
Disruption phenotypei
Non-essential; synthetic lethality is seen in a triple tig-dnaK-dnaJ disruption, although this depends on temperature (triple disruptions grow slowly at 20 and 34 degrees Celsius but not at all at 43 degrees) and strain background.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 32 | G → D in SEG-1 and dnaK756(TS); confers temperature sensitivity. 2 Publications | 1 | |
Mutagenesisi | 436 | V → I in SEG-2; confers temperature sensitivity. 1 Publication | 1 | |
Mutagenesisi | 455 | G → D in dnaK756(TS); confers temperature sensitivity. 1 Publication | 1 | |
Mutagenesisi | 468 | G → D in dnaK756(TS); confers temperature sensitivity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed3 Publications | |||
ChainiPRO_0000078458 | 2 – 638 | Chaperone protein DnaKAdd BLAST | 637 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 70 | N6-succinyllysine1 Publication | 1 | |
Modified residuei | 109 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 199 | Phosphothreonine; by autocatalysis2 Publications | 1 | |
Modified residuei | 245 | N6-acetyllysine; alternate1 Publication | 1 | |
Modified residuei | 245 | N6-succinyllysine; alternate1 Publication | 1 | |
Modified residuei | 246 | N6-succinyllysine1 Publication | 1 | |
Modified residuei | 304 | N6-acetyllysine; alternate1 Publication | 1 | |
Modified residuei | 304 | N6-succinyllysine; alternate1 Publication | 1 | |
Modified residuei | 359 | N6-succinyllysine1 Publication | 1 | |
Modified residuei | 421 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 502 | N6-succinyllysine1 Publication | 1 | |
Modified residuei | 528 | N6-succinyllysine1 Publication | 1 | |
Modified residuei | 556 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 587 | N6-succinyllysine1 Publication | 1 |
Post-translational modificationi
Autophosphorylated; GrpE inhibits the autophosphorylation.3 Publications
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P0A6Y8 |
PaxDbi | P0A6Y8 |
PRIDEi | P0A6Y8 |
2D gel databases
SWISS-2DPAGEi | P0A6Y8 |
PTM databases
CarbonylDBi | P0A6Y8 |
iPTMneti | P0A6Y8 |
Interactioni
Binary interactionsi
Hide detailsP0A6Y8
GO - Molecular functioni
- chaperone binding Source: CAFA
- unfolded protein binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 4259520, 182 interactors 849153, 11 interactors |
DIPi | DIP-35751N |
IntActi | P0A6Y8, 380 interactors |
MINTi | P0A6Y8 |
STRINGi | 511145.b0014 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A6Y8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A6Y8 |
Family & Domainsi
Sequence similaritiesi
Belongs to the heat shock protein 70 family.Curated
Phylogenomic databases
eggNOGi | COG0443, Bacteria |
HOGENOMi | CLU_005965_2_1_6 |
InParanoidi | P0A6Y8 |
PhylomeDBi | P0A6Y8 |
Family and domain databases
DisProti | DP02985 |
Gene3Di | 1.20.1270.10, 1 hit 2.60.34.10, 1 hit |
HAMAPi | MF_00332, DnaK, 1 hit |
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR012725, Chaperone_DnaK IPR018181, Heat_shock_70_CS IPR029048, HSP70_C_sf IPR029047, HSP70_peptide-bd_sf IPR013126, Hsp_70_fam |
PANTHERi | PTHR19375, PTHR19375, 1 hit |
Pfami | View protein in Pfam PF00012, HSP70, 1 hit |
SUPFAMi | SSF100920, SSF100920, 1 hit SSF100934, SSF100934, 1 hit SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR02350, prok_dnaK, 1 hit |
PROSITEi | View protein in PROSITE PS00297, HSP70_1, 1 hit PS00329, HSP70_2, 1 hit PS01036, HSP70_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A6Y8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV
60 70 80 90 100
GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD
110 120 130 140 150
AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ
160 170 180 190 200
RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF
210 220 230 240 250
DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI
260 270 280 290 300
DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV
310 320 330 340 350
TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ
360 370 380 390 400
KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTGDVKDVL LLDVTPLSLG
410 420 430 440 450
IETMGGVMTT LIAKNTTIPT KHSQVFSTAE DNQSAVTIHV LQGERKRAAD
460 470 480 490 500
NKSLGQFNLD GINPAPRGMP QIEVTFDIDA DGILHVSAKD KNSGKEQKIT
510 520 530 540 550
IKASSGLNED EIQKMVRDAE ANAEADRKFE ELVQTRNQGD HLLHSTRKQV
560 570 580 590 600
EEAGDKLPAD DKTAIESALT ALETALKGED KAAIEAKMQE LAQVSQKLME
610 620 630
IAQQQHAQQQ TAGADASANN AKDDDVVDAE FEEVKDKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01298 Genomic DNA Translation: AAA23694.1 U00096 Genomic DNA Translation: AAC73125.1 AP009048 Genomic DNA Translation: BAB96589.1 D10765 Genomic DNA Translation: BAA01595.1 M12565 Genomic DNA Translation: AAA23692.1 |
PIRi | A03311, IQECDK |
RefSeqi | NP_414555.1, NC_000913.3 WP_000516135.1, NZ_SSZK01000015.1 |
Genome annotation databases
EnsemblBacteriai | AAC73125; AAC73125; b0014 BAB96589; BAB96589; BAB96589 |
GeneIDi | 58463508 944750 |
KEGGi | ecj:JW0013 eco:b0014 |
PATRICi | fig|1411691.4.peg.2270 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01298 Genomic DNA Translation: AAA23694.1 U00096 Genomic DNA Translation: AAC73125.1 AP009048 Genomic DNA Translation: BAB96589.1 D10765 Genomic DNA Translation: BAA01595.1 M12565 Genomic DNA Translation: AAA23692.1 |
PIRi | A03311, IQECDK |
RefSeqi | NP_414555.1, NC_000913.3 WP_000516135.1, NZ_SSZK01000015.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BPR | NMR | - | A | 384-561 | [»] | |
1DG4 | NMR | - | A | 393-507 | [»] | |
1DKG | X-ray | 2.80 | D | 1-383 | [»] | |
1DKX | X-ray | 2.00 | A | 389-607 | [»] | |
1DKY | X-ray | 2.80 | A/B | 389-607 | [»] | |
1DKZ | X-ray | 2.00 | A | 389-607 | [»] | |
1Q5L | NMR | - | A | 393-507 | [»] | |
2BPR | NMR | - | A | 384-561 | [»] | |
2KHO | NMR | - | A | 1-605 | [»] | |
3DPO | X-ray | 2.10 | A/B | 389-607 | [»] | |
3DPP | X-ray | 2.50 | A/B | 389-607 | [»] | |
3DPQ | X-ray | 2.60 | A/B/E/F | 389-601 | [»] | |
3QNJ | X-ray | 2.28 | A/B | 389-607 | [»] | |
4B9Q | X-ray | 2.40 | A/B/C/D | 1-605 | [»] | |
4E81 | X-ray | 1.90 | A/B | 389-607 | [»] | |
4EZN | X-ray | 1.80 | A/B | 389-607 | [»] | |
4EZO | X-ray | 1.90 | A/B | 389-607 | [»] | |
4EZP | X-ray | 1.65 | A/B | 389-607 | [»] | |
4EZQ | X-ray | 2.00 | A | 389-607 | [»] | |
4EZR | X-ray | 1.90 | A | 389-607 | [»] | |
4EZS | X-ray | 1.90 | A | 389-607 | [»] | |
4EZT | X-ray | 2.00 | A | 389-607 | [»] | |
4EZU | X-ray | 1.90 | A | 389-607 | [»] | |
4EZV | X-ray | 2.10 | A/B | 389-607 | [»] | |
4EZW | X-ray | 1.80 | A/B/C/D | 389-607 | [»] | |
4EZX | X-ray | 1.70 | A/B | 389-607 | [»] | |
4EZY | X-ray | 1.85 | A | 389-607 | [»] | |
4EZZ | X-ray | 2.05 | A | 389-607 | [»] | |
4F00 | X-ray | 1.95 | A | 389-607 | [»] | |
4F01 | X-ray | 1.40 | A/B | 389-607 | [»] | |
4HY9 | X-ray | 1.55 | A/B | 389-607 | [»] | |
4HYB | X-ray | 1.70 | A/B | 389-607 | [»] | |
4JN4 | X-ray | 2.30 | A/B | 2-610 | [»] | |
4JNE | X-ray | 1.96 | A/B | 2-610 | [»] | |
4JNF | X-ray | 1.62 | A | 389-610 | [»] | |
4JWC | X-ray | 1.80 | A/B | 389-607 | [»] | |
4JWD | X-ray | 1.95 | A/B | 389-607 | [»] | |
4JWE | X-ray | 1.95 | A/B | 389-607 | [»] | |
4JWI | X-ray | 1.90 | A/B | 389-607 | [»] | |
4R5G | X-ray | 3.45 | A/B | 389-607 | [»] | |
4R5I | X-ray | 1.97 | A | 389-607 | [»] | |
4R5J | X-ray | 2.36 | A/B/C/D | 389-607 | [»] | |
4R5K | X-ray | 1.75 | A/B | 389-607 | [»] | |
4R5L | X-ray | 2.97 | A/B/C/D | 389-607 | [»] | |
5NRO | X-ray | 3.25 | A | 1-605 | [»] | |
5OOW | X-ray | 2.90 | A/B | 183-383 | [»] | |
7JM8 | X-ray | 2.00 | A | 389-607 | [»] | |
7JML | X-ray | 2.55 | A | 389-607 | [»] | |
7JMM | X-ray | 2.56 | A | 389-607 | [»] | |
7JMZ | X-ray | 2.42 | A | 389-607 | [»] | |
7JN8 | X-ray | 3.09 | A | 389-607 | [»] | |
7JN9 | X-ray | 2.40 | A | 389-607 | [»] | |
7JNE | X-ray | 2.54 | A | 389-607 | [»] | |
7JNG | X-ray | 2.80 | A | 389-607 | [»] | |
SMRi | P0A6Y8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259520, 182 interactors 849153, 11 interactors |
DIPi | DIP-35751N |
IntActi | P0A6Y8, 380 interactors |
MINTi | P0A6Y8 |
STRINGi | 511145.b0014 |
Protein family/group databases
TCDBi | 1.A.33.1.2, the cation channel-forming heat shock protein-70 (hsp70) family |
PTM databases
CarbonylDBi | P0A6Y8 |
iPTMneti | P0A6Y8 |
2D gel databases
SWISS-2DPAGEi | P0A6Y8 |
Proteomic databases
jPOSTi | P0A6Y8 |
PaxDbi | P0A6Y8 |
PRIDEi | P0A6Y8 |
Genome annotation databases
EnsemblBacteriai | AAC73125; AAC73125; b0014 BAB96589; BAB96589; BAB96589 |
GeneIDi | 58463508 944750 |
KEGGi | ecj:JW0013 eco:b0014 |
PATRICi | fig|1411691.4.peg.2270 |
Organism-specific databases
EchoBASEi | EB0237 |
Phylogenomic databases
eggNOGi | COG0443, Bacteria |
HOGENOMi | CLU_005965_2_1_6 |
InParanoidi | P0A6Y8 |
PhylomeDBi | P0A6Y8 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10241-MONOMER MetaCyc:EG10241-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A6Y8 |
PROi | PR:P0A6Y8 |
Family and domain databases
DisProti | DP02985 |
Gene3Di | 1.20.1270.10, 1 hit 2.60.34.10, 1 hit |
HAMAPi | MF_00332, DnaK, 1 hit |
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR012725, Chaperone_DnaK IPR018181, Heat_shock_70_CS IPR029048, HSP70_C_sf IPR029047, HSP70_peptide-bd_sf IPR013126, Hsp_70_fam |
PANTHERi | PTHR19375, PTHR19375, 1 hit |
Pfami | View protein in Pfam PF00012, HSP70, 1 hit |
SUPFAMi | SSF100920, SSF100920, 1 hit SSF100934, SSF100934, 1 hit SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR02350, prok_dnaK, 1 hit |
PROSITEi | View protein in PROSITE PS00297, HSP70_1, 1 hit PS00329, HSP70_2, 1 hit PS01036, HSP70_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DNAK_ECOLI | |
Accessioni | P0A6Y8Primary (citable) accession number: P0A6Y8 Secondary accession number(s): P04475 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families