UniProtKB - P0A6Y5 (HSLO_ECOLI)
Protein
33 kDa chaperonin
Gene
hslO
Organism
Escherichia coli (strain K12)
Status
Functioni
Redox regulated molecular chaperone. Protects both thermally unfolding and oxidatively damaged proteins from irreversible aggregation. Plays an important role in the bacterial defense system toward oxidative stress.
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein folding chaperone Source: EcoCyc
- unfolded protein binding Source: UniProtKB-UniRule
- zinc ion binding Source: EcoCyc
GO - Biological processi
- maintenance of unfolded protein Source: EcoCyc
- protein refolding Source: EcoCyc
- response to heat Source: EcoliWiki
- response to oxidative stress Source: EcoCyc
Keywordsi
Molecular function | Chaperone |
Biological process | Stress response |
Ligand | Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G7744-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: 33 kDa chaperoninUniRule annotationAlternative name(s): Heat shock protein 33 Short name: HSP33UniRule annotation |
Gene namesi | Name:hslOUniRule annotation Synonyms:yrfI Ordered Locus Names:b3401, JW5692 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192174 | 1 – 292 | 33 kDa chaperoninAdd BLAST | 292 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 230 ↔ 232 | Redox-activeUniRule annotation1 Publication | ||
Disulfide bondi | 263 ↔ 266 | Redox-activeUniRule annotation1 Publication |
Post-translational modificationi
Under oxidizing conditions two disulfide bonds are formed involving the reactive cysteines. Under reducing conditions zinc is bound to the reactive cysteines and the protein is inactive.
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P0A6Y5 |
PaxDbi | P0A6Y5 |
PRIDEi | P0A6Y5 |
Expressioni
Inductioni
By heat shock.
Interactioni
Binary interactionsi
Hide detailsP0A6Y5
With | #Exp. | IntAct |
---|---|---|
itself | 5 | EBI-562857,EBI-562857 |
tufA [P0CE47] | 3 | EBI-562857,EBI-301077 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- unfolded protein binding Source: UniProtKB-UniRule
Protein-protein interaction databases
BioGRIDi | 4262486, 11 interactors |
DIPi | DIP-48013N |
IntActi | P0A6Y5, 11 interactors |
MINTi | P0A6Y5 |
STRINGi | 511145.b3401 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A6Y5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A6Y5 |
Family & Domainsi
Sequence similaritiesi
Belongs to the HSP33 family.UniRule annotation
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | COG1281, Bacteria |
HOGENOMi | CLU_054493_0_0_6 |
InParanoidi | P0A6Y5 |
PhylomeDBi | P0A6Y5 |
Family and domain databases
CDDi | cd00498, Hsp33, 1 hit |
Gene3Di | 1.10.287.480, 1 hit 3.55.30.10, 1 hit 3.90.1280.10, 1 hit |
HAMAPi | MF_00117, HslO, 1 hit |
InterProi | View protein in InterPro IPR000397, Heat_shock_Hsp33 IPR016154, Heat_shock_Hsp33_C IPR016153, Heat_shock_Hsp33_N IPR023212, Hsp33_helix_hairpin_bin_dom_sf |
PANTHERi | PTHR30111, PTHR30111, 1 hit |
Pfami | View protein in Pfam PF01430, HSP33, 1 hit |
PIRSFi | PIRSF005261, Heat_shock_Hsp33, 1 hit |
SUPFAMi | SSF118352, SSF118352, 1 hit SSF64397, SSF64397, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A6Y5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPQHDQLHRY LFENFAVRGE LVTVSETLQQ ILENHDYPQP VKNVLAELLV
60 70 80 90 100
ATSLLTATLK FDGDITVQLQ GDGPMNLAVI NGNNNQQMRG VARVQGEIPE
110 120 130 140 150
NADLKTLVGN GYVVITITPS EGERYQGVVG LEGDTLAACL EDYFMRSEQL
160 170 180 190 200
PTRLFIRTGD VDGKPAAGGM LLQVMPAQNA QQDDFDHLAT LTETIKTEEL
210 220 230 240 250
LTLPANEVLW RLYHEEEVTV YDPQDVEFKC TCSRERCADA LKTLPDEEVD
260 270 280 290
SILAEDGEID MHCDYCGNHY LFNAMDIAEI RNNASPADPQ VH
Sequence cautioni
The sequence AAA58198 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58198.1 Different initiation. U00096 Genomic DNA Translation: AAC76426.2 AP009048 Genomic DNA Translation: BAE77890.1 |
PIRi | D65135 |
RefSeqi | NP_417860.2, NC_000913.3 WP_001135574.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76426; AAC76426; b3401 BAE77890; BAE77890; BAE77890 |
GeneIDi | 57729335 947178 |
KEGGi | ecj:JW5692 eco:b3401 |
PATRICi | fig|1411691.4.peg.3329 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58198.1 Different initiation. U00096 Genomic DNA Translation: AAC76426.2 AP009048 Genomic DNA Translation: BAE77890.1 |
PIRi | D65135 |
RefSeqi | NP_417860.2, NC_000913.3 WP_001135574.1, NZ_STEB01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HW7 | X-ray | 2.20 | A | 1-253 | [»] | |
1I7F | X-ray | 2.70 | A | 1-292 | [»] | |
1XJH | NMR | - | A | 225-285 | [»] | |
3M7M | X-ray | 2.90 | X | 1-233 | [»] | |
SMRi | P0A6Y5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262486, 11 interactors |
DIPi | DIP-48013N |
IntActi | P0A6Y5, 11 interactors |
MINTi | P0A6Y5 |
STRINGi | 511145.b3401 |
Chemistry databases
DrugBanki | DB03814, 2-(N-morpholino)ethanesulfonic acid |
Proteomic databases
jPOSTi | P0A6Y5 |
PaxDbi | P0A6Y5 |
PRIDEi | P0A6Y5 |
Genome annotation databases
EnsemblBacteriai | AAC76426; AAC76426; b3401 BAE77890; BAE77890; BAE77890 |
GeneIDi | 57729335 947178 |
KEGGi | ecj:JW5692 eco:b3401 |
PATRICi | fig|1411691.4.peg.3329 |
Organism-specific databases
EchoBASEi | EB2766 |
Phylogenomic databases
eggNOGi | COG1281, Bacteria |
HOGENOMi | CLU_054493_0_0_6 |
InParanoidi | P0A6Y5 |
PhylomeDBi | P0A6Y5 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7744-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A6Y5 |
PROi | PR:P0A6Y5 |
Family and domain databases
CDDi | cd00498, Hsp33, 1 hit |
Gene3Di | 1.10.287.480, 1 hit 3.55.30.10, 1 hit 3.90.1280.10, 1 hit |
HAMAPi | MF_00117, HslO, 1 hit |
InterProi | View protein in InterPro IPR000397, Heat_shock_Hsp33 IPR016154, Heat_shock_Hsp33_C IPR016153, Heat_shock_Hsp33_N IPR023212, Hsp33_helix_hairpin_bin_dom_sf |
PANTHERi | PTHR30111, PTHR30111, 1 hit |
Pfami | View protein in Pfam PF01430, HSP33, 1 hit |
PIRSFi | PIRSF005261, Heat_shock_Hsp33, 1 hit |
SUPFAMi | SSF118352, SSF118352, 1 hit SSF64397, SSF64397, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HSLO_ECOLI | |
Accessioni | P0A6Y5Primary (citable) accession number: P0A6Y5 Secondary accession number(s): P45803, Q2M766 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | April 7, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families