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Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA. Requires the intact 30S subunit for methylation.UniRule annotation1 Publication

Miscellaneous

Mechanisms acting on transcription regulation, translation or protein stability could be responsible for adjusting RsmG expression to rRNA synthesis.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N7-methylguanine(527) in 16S rRNA.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei78S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei139S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

  • rRNA (guanine-N7-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • rRNA base methylation Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10376-MONOMER
MetaCyc:EG10376-MONOMER
BRENDAi2.1.1.170 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.170UniRule annotation)
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
Short name:
16S rRNA m7G methyltransferaseUniRule annotation
Glucose-inhibited division protein B
Gene namesi
Name:rsmGUniRule annotation
Synonyms:gidB
Ordered Locus Names:b3740, JW3718
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10376 rsmG

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Low-level streptomycin resistance.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39N → A: Lack of activity. Low-level streptomycin resistance. Slight decrease in AdoMet affinity. 1 Publication1
Mutagenesisi53H → A: Decrease in activity. Very low-level streptomycin resistance. Slight decrease in AdoMet affinity. 1 Publication1
Mutagenesisi56D → A: Strong decrease in activity. Low-level streptomycin resistance. Slight decrease in AdoMet affinity. 1 Publication1
Mutagenesisi71D → A: Lack of activity. Low-level streptomycin resistance. 10-fold increase in AdoMet affinity. 1 Publication1
Mutagenesisi73G → A: Slight decrease in activity. Streptomycin-sensitive. Slight decrease in AdoMet affinity. 1 Publication1
Mutagenesisi75G → A: Lack of activity. Low-level streptomycin resistance. 24-fold decrease in AdoMet affinity. 1 Publication1
Mutagenesisi77G → A: Lack of activity. Low-level streptomycin resistance. 10-fold decrease in AdoMet affinity. 1 Publication1
Mutagenesisi79P → A: Lack of activity. Low-level streptomycin resistance. 11-fold decrease in AdoMet affinity. 1 Publication1
Mutagenesisi96D → A: Lack of activity. Low-level streptomycin resistance. 1 Publication1
Mutagenesisi100 – 101KR → AA: Lack of activity. Low-level streptomycin resistance. 1 Publication2
Mutagenesisi123R → A: Slight decrease in activity. Streptomycin-sensitive. 1 Publication1
Mutagenesisi139R → A: Lack of activity. Low-level streptomycin resistance. No change in AdoMet affinity. 1 Publication1
Mutagenesisi139R → K: Decrease in activity. Very low-level streptomycin resistance. 1 Publication1
Mutagenesisi165K → A: Slight decrease in activity. Streptomycin-sensitive. 1 Publication1
Mutagenesisi197R → A: Strong decrease in activity. Low-level streptomycin resistance. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001842491 – 207Ribosomal RNA small subunit methyltransferase GAdd BLAST207

Proteomic databases

EPDiP0A6U5
PaxDbiP0A6U5
PRIDEiP0A6U5

Expressioni

Inductioni

Induced under stress conditions known to stimulate ppGpp accumulation and down-regulate rRNA synthesis.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263261, 260 interactors
DIPiDIP-47992N
IntActiP0A6U5, 10 interactors
STRINGi316385.ECDH10B_3927

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0A6U5
SMRiP0A6U5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6U5

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D28 Bacteria
COG0357 LUCA
HOGENOMiHOG000221012
InParanoidiP0A6U5
KOiK03501
OMAiYPRKAGI
PhylomeDBiP0A6U5

Family and domain databases

HAMAPiMF_00074 16SrRNA_methyltr_G, 1 hit
InterProiView protein in InterPro
IPR003682 rRNA_ssu_MeTfrase_G
IPR029063 SAM-dependent_MTases
PANTHERiPTHR31760 PTHR31760, 1 hit
PfamiView protein in Pfam
PF02527 GidB, 1 hit
PIRSFiPIRSF003078 GidB, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00138 rsmG_gidB, 1 hit

Sequencei

Sequence statusi: Complete.

P0A6U5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLNKLSLLLK DAGISLTDHQ KNQLIAYVNM LHKWNKAYNL TSVRDPNEML
60 70 80 90 100
VRHILDSIVV APYLQGERFI DVGTGPGLPG IPLSIVRPEA HFTLLDSLGK
110 120 130 140 150
RVRFLRQVQH ELKLENIEPV QSRVEEFPSE PPFDGVISRA FASLNDMVSW
160 170 180 190 200
CHHLPGEQGR FYALKGQMPE DEIALLPEEY QVESVVKLQV PALDGERHLV

VIKANKI
Length:207
Mass (Da):23,431
Last modified:March 29, 2005 - v1
Checksum:i40CF9BA134E9A234
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA Translation: CAA25774.1
L10328 Genomic DNA Translation: AAA62092.1
U00096 Genomic DNA Translation: AAC76763.1
AP009048 Genomic DNA Translation: BAE77548.1
X01383 Genomic DNA Translation: CAA25639.1
PIRiC30389 BVECQB
RefSeqiNP_418196.1, NC_000913.3
WP_000932839.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76763; AAC76763; b3740
BAE77548; BAE77548; BAE77548
GeneIDi948250
KEGGiecj:JW3718
eco:b3740
PATRICifig|1411691.4.peg.2960

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA Translation: CAA25774.1
L10328 Genomic DNA Translation: AAA62092.1
U00096 Genomic DNA Translation: AAC76763.1
AP009048 Genomic DNA Translation: BAE77548.1
X01383 Genomic DNA Translation: CAA25639.1
PIRiC30389 BVECQB
RefSeqiNP_418196.1, NC_000913.3
WP_000932839.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JSXX-ray2.40A1-207[»]
ProteinModelPortaliP0A6U5
SMRiP0A6U5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263261, 260 interactors
DIPiDIP-47992N
IntActiP0A6U5, 10 interactors
STRINGi316385.ECDH10B_3927

Proteomic databases

EPDiP0A6U5
PaxDbiP0A6U5
PRIDEiP0A6U5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76763; AAC76763; b3740
BAE77548; BAE77548; BAE77548
GeneIDi948250
KEGGiecj:JW3718
eco:b3740
PATRICifig|1411691.4.peg.2960

Organism-specific databases

EchoBASEiEB0371
EcoGeneiEG10376 rsmG

Phylogenomic databases

eggNOGiENOG4105D28 Bacteria
COG0357 LUCA
HOGENOMiHOG000221012
InParanoidiP0A6U5
KOiK03501
OMAiYPRKAGI
PhylomeDBiP0A6U5

Enzyme and pathway databases

BioCyciEcoCyc:EG10376-MONOMER
MetaCyc:EG10376-MONOMER
BRENDAi2.1.1.170 2026

Miscellaneous databases

EvolutionaryTraceiP0A6U5
PROiPR:P0A6U5

Family and domain databases

HAMAPiMF_00074 16SrRNA_methyltr_G, 1 hit
InterProiView protein in InterPro
IPR003682 rRNA_ssu_MeTfrase_G
IPR029063 SAM-dependent_MTases
PANTHERiPTHR31760 PTHR31760, 1 hit
PfamiView protein in Pfam
PF02527 GidB, 1 hit
PIRSFiPIRSF003078 GidB, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00138 rsmG_gidB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRSMG_ECOLI
AccessioniPrimary (citable) accession number: P0A6U5
Secondary accession number(s): P17113, Q2M858
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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