UniProtKB - P0A6T9 (GCSH_ECOLI)
Protein
Glycine cleavage system H protein
Gene
gcvH
Organism
Escherichia coli (strain K12)
Status
Functioni
The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.UniRule annotation1 Publication
Cofactori
(R)-lipoateUniRule annotationNote: Binds 1 lipoyl cofactor covalently.UniRule annotation
GO - Molecular functioni
- lipoic acid binding Source: EcoliWiki
GO - Biological processi
- glycine decarboxylation via glycine cleavage system Source: EcoliWiki
- protein lipoylation Source: GO_Central
Enzyme and pathway databases
BioCyci | EcoCyc:GCVH-MONOMER MetaCyc:GCVH-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Glycine cleavage system H proteinUniRule annotation |
Gene namesi | Name:gcvH1 PublicationUniRule annotation Ordered Locus Names:b2904, JW2872 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: EcoCyc
Other locations
- cytoplasm Source: EcoliWiki
- glycine cleavage complex Source: InterPro
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000166218 | 2 – 129 | Glycine cleavage system H proteinAdd BLAST | 128 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 65 | N6-lipoyllysineUniRule annotation | 1 |
Proteomic databases
jPOSTi | P0A6T9 |
PaxDbi | P0A6T9 |
PRIDEi | P0A6T9 |
Interactioni
Subunit structurei
The glycine cleavage system is composed of four proteins: P, T, L and H.
UniRule annotation1 PublicationProtein-protein interaction databases
BioGRIDi | 4261019, 43 interactors 851715, 2 interactors |
ComplexPortali | CPX-3949, Glycine cleavage system complex |
IntActi | P0A6T9, 2 interactors |
STRINGi | 511145.b2904 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A6T9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A6T9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 106 | Lipoyl-bindingUniRule annotationAdd BLAST | 83 |
Sequence similaritiesi
Belongs to the GcvH family.UniRule annotation
Keywords - Domaini
LipoylPhylogenomic databases
eggNOGi | COG0509, Bacteria |
HOGENOMi | CLU_097408_2_1_6 |
InParanoidi | P0A6T9 |
PhylomeDBi | P0A6T9 |
Family and domain databases
CDDi | cd06848, GCS_H, 1 hit |
HAMAPi | MF_00272, GcvH, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR000089, Biotin_lipoyl IPR002930, GCV_H IPR033753, GCV_H/Fam206 IPR017453, GCV_H_sub IPR011053, Single_hybrid_motif |
PANTHERi | PTHR11715, PTHR11715, 1 hit |
Pfami | View protein in Pfam PF01597, GCV_H, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit |
TIGRFAMsi | TIGR00527, gcvH, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 1 hit PS00189, LIPOYL, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A6T9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNVPAELKY SKEHEWLRKE ADGTYTVGIT EHAQELLGDM VFVDLPEVGA
60 70 80 90 100
TVSAGDDCAV AESVKAASDI YAPVSGEIVA VNDALSDSPE LVNSEPYAGG
110 120
WIFKIKASDE SELESLLDAT AYEALLEDE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M57690 Genomic DNA Translation: AAA68887.1 X73958 Genomic DNA Translation: CAA52145.1 U28377 Genomic DNA Translation: AAA69072.1 U00096 Genomic DNA Translation: AAC75942.1 M97263 Unassigned DNA Translation: AAC36844.1 L20872 Genomic DNA Translation: AAA23866.1 AP009048 Genomic DNA Translation: BAE76969.1 |
PIRi | A56623 |
RefSeqi | NP_417380.1, NC_000913.3 WP_001295377.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC75942; AAC75942; b2904 BAE76969; BAE76969; BAE76969 |
GeneIDi | 58460566 947393 |
KEGGi | ecj:JW2872 eco:b2904 |
PATRICi | fig|1411691.4.peg.3828 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M57690 Genomic DNA Translation: AAA68887.1 X73958 Genomic DNA Translation: CAA52145.1 U28377 Genomic DNA Translation: AAA69072.1 U00096 Genomic DNA Translation: AAC75942.1 M97263 Unassigned DNA Translation: AAC36844.1 L20872 Genomic DNA Translation: AAA23866.1 AP009048 Genomic DNA Translation: BAE76969.1 |
PIRi | A56623 |
RefSeqi | NP_417380.1, NC_000913.3 WP_001295377.1, NZ_STEB01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3A7A | X-ray | 3.10 | B/D | 2-129 | [»] | |
3A7L | X-ray | 1.30 | A | 2-129 | [»] | |
3A8I | X-ray | 1.99 | E/F | 1-129 | [»] | |
3A8J | X-ray | 1.98 | E/F | 1-129 | [»] | |
3A8K | X-ray | 1.95 | E/F | 1-129 | [»] | |
3AB9 | X-ray | 1.65 | A | 1-129 | [»] | |
SMRi | P0A6T9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261019, 43 interactors 851715, 2 interactors |
ComplexPortali | CPX-3949, Glycine cleavage system complex |
IntActi | P0A6T9, 2 interactors |
STRINGi | 511145.b2904 |
Proteomic databases
jPOSTi | P0A6T9 |
PaxDbi | P0A6T9 |
PRIDEi | P0A6T9 |
Genome annotation databases
EnsemblBacteriai | AAC75942; AAC75942; b2904 BAE76969; BAE76969; BAE76969 |
GeneIDi | 58460566 947393 |
KEGGi | ecj:JW2872 eco:b2904 |
PATRICi | fig|1411691.4.peg.3828 |
Organism-specific databases
EchoBASEi | EB0366 |
Phylogenomic databases
eggNOGi | COG0509, Bacteria |
HOGENOMi | CLU_097408_2_1_6 |
InParanoidi | P0A6T9 |
PhylomeDBi | P0A6T9 |
Enzyme and pathway databases
BioCyci | EcoCyc:GCVH-MONOMER MetaCyc:GCVH-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A6T9 |
PROi | PR:P0A6T9 |
Family and domain databases
CDDi | cd06848, GCS_H, 1 hit |
HAMAPi | MF_00272, GcvH, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR000089, Biotin_lipoyl IPR002930, GCV_H IPR033753, GCV_H/Fam206 IPR017453, GCV_H_sub IPR011053, Single_hybrid_motif |
PANTHERi | PTHR11715, PTHR11715, 1 hit |
Pfami | View protein in Pfam PF01597, GCV_H, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit |
TIGRFAMsi | TIGR00527, gcvH, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 1 hit PS00189, LIPOYL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GCSH_ECOLI | |
Accessioni | P0A6T9Primary (citable) accession number: P0A6T9 Secondary accession number(s): P23884, Q2M9T7, Q8Z3W9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families