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Protein

GTP cyclohydrolase 1

Gene

folE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

Activity regulationi

Allosteric enzyme. Activity is modulated by K+, divalent cations, UTP, and tetrahydrobiopterin. Tetrahydrobiopterin is an inhibitor of this enzyme.

Pathwayi: 7,8-dihydroneopterin triphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP.
Proteins known to be involved in this subpathway in this organism are:
  1. GTP cyclohydrolase 1 (folE)
This subpathway is part of the pathway 7,8-dihydroneopterin triphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP, the pathway 7,8-dihydroneopterin triphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi111Zinc1
Metal bindingi114Zinc1
Metal bindingi182Zinc1

GO - Molecular functioni

  • GTP binding Source: EcoliWiki
  • GTP cyclohydrolase I activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • 7,8-dihydroneopterin 3'-triphosphate biosynthetic process Source: UniProtKB-UniPathway
  • one-carbon metabolic process Source: UniProtKB-UniRule
  • queuosine biosynthetic process Source: EcoCyc
  • tetrahydrobiopterin biosynthetic process Source: GO_Central
  • tetrahydrofolate biosynthetic process Source: UniProtKB-UniRule

Keywordsi

Molecular functionAllosteric enzyme, Hydrolase
Biological processOne-carbon metabolism
LigandGTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:GTP-CYCLOHYDRO-I-MONOMER
MetaCyc:GTP-CYCLOHYDRO-I-MONOMER
BRENDAi3.5.4.16 2026
UniPathwayi
UPA00848;UER00151

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 (EC:3.5.4.16)
Alternative name(s):
GTP cyclohydrolase I
Short name:
GTP-CH-I
Gene namesi
Name:folE
Ordered Locus Names:b2153, JW2140
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11375 folE

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001194042 – 222GTP cyclohydrolase 1Add BLAST221

Proteomic databases

EPDiP0A6T5
PaxDbiP0A6T5
PRIDEiP0A6T5

Interactioni

Subunit structurei

Toroid-shaped homodecamer, composed of two pentamers of five dimers.2 Publications

Protein-protein interaction databases

BioGridi4263524, 298 interactors
853284, 1 interactor
DIPiDIP-9676N
IntActiP0A6T5, 1 interactor
MINTiP0A6T5
STRINGi316385.ECDH10B_2310

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0A6T5
SMRiP0A6T5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6T5

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family.Curated

Phylogenomic databases

eggNOGiENOG4105EUJ Bacteria
COG0302 LUCA
HOGENOMiHOG000221222
InParanoidiP0A6T5
KOiK01495
PhylomeDBiP0A6T5

Family and domain databases

HAMAPiMF_00223 FolE, 1 hit
InterProiView protein in InterPro
IPR001474 GTP_CycHdrlase_I
IPR018234 GTP_CycHdrlase_I_CS
IPR020602 GTP_CycHdrlase_I_dom
PANTHERiPTHR11109 PTHR11109, 1 hit
PfamiView protein in Pfam
PF01227 GTP_cyclohydroI, 1 hit
TIGRFAMsiTIGR00063 folE, 1 hit
PROSITEiView protein in PROSITE
PS00859 GTP_CYCLOHYDROL_1_1, 1 hit
PS00860 GTP_CYCLOHYDROL_1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A6T5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPSLSKEAAL VHEALVARGL ETPLRPPVHE MDNETRKSLI AGHMTEIMQL
60 70 80 90 100
LNLDLADDSL METPHRIAKM YVDEIFSGLD YANFPKITLI ENKMKVDEMV
110 120 130 140 150
TVRDITLTST CEHHFVTIDG KATVAYIPKD SVIGLSKINR IVQFFAQRPQ
160 170 180 190 200
VQERLTQQIL IALQTLLGTN NVAVSIDAVH YCVKARGIRD ATSATTTTSL
210 220
GGLFKSSQNT RHEFLRAVRH HN
Length:222
Mass (Da):24,831
Last modified:January 23, 2007 - v2
Checksum:i23C6A1440F579FC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63910 Genomic DNA Translation: CAA45365.1
U00007 Genomic DNA Translation: AAA60535.1
U00096 Genomic DNA Translation: AAC75214.1
AP009048 Genomic DNA Translation: BAE76630.1
PIRiH64983
S29895
RefSeqiNP_416658.1, NC_000913.3
WP_001139613.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75214; AAC75214; b2153
BAE76630; BAE76630; BAE76630
GeneIDi949040
KEGGiecj:JW2140
eco:b2153
PATRICifig|1411691.4.peg.88

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63910 Genomic DNA Translation: CAA45365.1
U00007 Genomic DNA Translation: AAA60535.1
U00096 Genomic DNA Translation: AAC75214.1
AP009048 Genomic DNA Translation: BAE76630.1
PIRiH64983
S29895
RefSeqiNP_416658.1, NC_000913.3
WP_001139613.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A8RX-ray2.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1A9CX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1FBXX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1GTPX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T2-222[»]
1N3RX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
1N3SX-ray2.55A/B/C/D/E/F/G/H/I/J2-222[»]
1N3TX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L/M/N/O2-222[»]
ProteinModelPortaliP0A6T5
SMRiP0A6T5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263524, 298 interactors
853284, 1 interactor
DIPiDIP-9676N
IntActiP0A6T5, 1 interactor
MINTiP0A6T5
STRINGi316385.ECDH10B_2310

Proteomic databases

EPDiP0A6T5
PaxDbiP0A6T5
PRIDEiP0A6T5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75214; AAC75214; b2153
BAE76630; BAE76630; BAE76630
GeneIDi949040
KEGGiecj:JW2140
eco:b2153
PATRICifig|1411691.4.peg.88

Organism-specific databases

EchoBASEiEB1349
EcoGeneiEG11375 folE

Phylogenomic databases

eggNOGiENOG4105EUJ Bacteria
COG0302 LUCA
HOGENOMiHOG000221222
InParanoidiP0A6T5
KOiK01495
PhylomeDBiP0A6T5

Enzyme and pathway databases

UniPathwayi
UPA00848;UER00151

BioCyciEcoCyc:GTP-CYCLOHYDRO-I-MONOMER
MetaCyc:GTP-CYCLOHYDRO-I-MONOMER
BRENDAi3.5.4.16 2026

Miscellaneous databases

EvolutionaryTraceiP0A6T5
PROiPR:P0A6T5

Family and domain databases

HAMAPiMF_00223 FolE, 1 hit
InterProiView protein in InterPro
IPR001474 GTP_CycHdrlase_I
IPR018234 GTP_CycHdrlase_I_CS
IPR020602 GTP_CycHdrlase_I_dom
PANTHERiPTHR11109 PTHR11109, 1 hit
PfamiView protein in Pfam
PF01227 GTP_cyclohydroI, 1 hit
TIGRFAMsiTIGR00063 folE, 1 hit
PROSITEiView protein in PROSITE
PS00859 GTP_CYCLOHYDROL_1_1, 1 hit
PS00860 GTP_CYCLOHYDROL_1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGCH1_ECOLI
AccessioniPrimary (citable) accession number: P0A6T5
Secondary accession number(s): P27511, Q2MAS6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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