UniProtKB - P0A6T3 (GAL1_ECOLI)
Protein
Galactokinase
Gene
galK
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P). To a lesser extent, is also able to phosphorylate 2-deoxy-D-galactose and D-galactosamine. Is not able to use D-galacturonic acid, D-talose, L-altrose, and L-glucose as substrates.UniRule annotation3 Publications
Catalytic activityi
- EC:2.7.1.6UniRule annotation3 Publications
Kineticsi
kcat is 108 min(-1), 30 min(-1) and 11.7 min(-1) with D-galactose, 2-deoxy-D-galactose, and D-galactosamine as substrate, respectively.
- KM=2.1 mM for D-galactose2 Publications
- KM=3.6 mM for 2-deoxy-D-galactose2 Publications
- KM=2.9 mM for D-galactosamine2 Publications
- KM=2.5 mM for ATP2 Publications
- Vmax=1.65 µmol/min/mg enzyme with D-galactose as substrate2 Publications
: galactose metabolism Pathwayi
This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 28 | Transition state stabilizerUniRule annotation | 1 | |
Metal bindingi | 130 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 162 | MagnesiumUniRule annotation | 1 | |
Active sitei | 174 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 223 | SubstrateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 124 – 130 | ATPUniRule annotation | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- galactokinase activity Source: EcoCyc
- magnesium ion binding Source: EcoCyc
GO - Biological processi
- galactose catabolic process via UDP-galactose Source: EcoCyc
- galactose metabolic process Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Carbohydrate metabolism, Galactose metabolism |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:GALACTOKIN-MONOMER MetaCyc:GALACTOKIN-MONOMER |
SABIO-RKi | P0A6T3 |
UniPathwayi | UPA00214 |
Names & Taxonomyi
Protein namesi | Recommended name: GalactokinaseUniRule annotation (EC:2.7.1.6UniRule annotation)Alternative name(s): Galactose kinaseUniRule annotation |
Gene namesi | Name:galKUniRule annotation Synonyms:galA Ordered Locus Names:b0757, JW0740 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 371 | Y → H: Displays relaxed substrate specificity since it gains kinase activity toward sugars as diverse as D-galacturonic acid, D-talose, L-altrose, and L-glucose. Also shows enhanced turnover of the small pool of sugars converted by the wild-type enzyme. 1 Publication | 1 |
Miscellaneous databases
PHI-basei | PHI:6269 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000184607 | 2 – 382 | GalactokinaseAdd BLAST | 381 |
Proteomic databases
jPOSTi | P0A6T3 |
PaxDbi | P0A6T3 |
PRIDEi | P0A6T3 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261711, 225 interactors 849734, 4 interactors |
IntActi | P0A6T3, 4 interactors |
STRINGi | 511145.b0757 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 37 | Substrate bindingUniRule annotation | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0153, Bacteria |
HOGENOMi | CLU_017814_2_1_6 |
InParanoidi | P0A6T3 |
PhylomeDBi | P0A6T3 |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit 3.30.70.890, 1 hit |
HAMAPi | MF_00246, Galactokinase, 1 hit |
InterProi | View protein in InterPro IPR000705, Galactokinase IPR022963, Galactokinase_bac IPR019741, Galactokinase_CS IPR019539, GalKase_N IPR013750, GHMP_kinase_C_dom IPR036554, GHMP_kinase_C_sf IPR006204, GHMP_kinase_N_dom IPR006203, GHMP_knse_ATP-bd_CS IPR006206, Mevalonate/galactokinase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr |
PANTHERi | PTHR10457:SF6, PTHR10457:SF6, 1 hit |
Pfami | View protein in Pfam PF10509, GalKase_gal_bdg, 1 hit PF08544, GHMP_kinases_C, 1 hit PF00288, GHMP_kinases_N, 1 hit |
PIRSFi | PIRSF000530, Galactokinase, 1 hit |
PRINTSi | PR00473, GALCTOKINASE |
SUPFAMi | SSF54211, SSF54211, 1 hit SSF55060, SSF55060, 1 hit |
TIGRFAMsi | TIGR00131, gal_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00106, GALACTOKINASE, 1 hit PS00627, GHMP_KINASES_ATP, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A6T3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLKEKTQSL FANAFGYPAT HTIQAPGRVN LIGEHTDYND GFVLPCAIDY
60 70 80 90 100
QTVISCAPRD DRKVRVMAAD YENQLDEFSL DAPIVAHENY QWANYVRGVV
110 120 130 140 150
KHLQLRNNSF GGVDMVISGN VPQGAGLSSS ASLEVAVGTV LQQLYHLPLD
160 170 180 190 200
GAQIALNGQE AENQFVGCNC GIMDQLISAL GKKDHALLID CRSLGTKAVS
210 220 230 240 250
MPKGVAVVII NSNFKRTLVG SEYNTRREQC ETGARFFQQP ALRDVTIEEF
260 270 280 290 300
NAVAHELDPI VAKRVRHILT ENARTVEAAS ALEQGDLKRM GELMAESHAS
310 320 330 340 350
MRDDFEITVP QIDTLVEIVK AVIGDKGGVR MTGGGFGGCI VALIPEELVP
360 370 380
AVQQAVAEQY EAKTGIKETF YVCKPSQGAG QC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02306 Genomic DNA Translation: CAA26172.1 U00096 Genomic DNA Translation: AAC73844.1 AP009048 Genomic DNA Translation: BAA35419.1 U13636 Genomic DNA Translation: AAB17019.1 |
PIRi | B23044, KIECGG |
RefSeqi | NP_415278.1, NC_000913.3 WP_000053415.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC73844; AAC73844; b0757 BAA35419; BAA35419; BAA35419 |
GeneIDi | 945358 |
KEGGi | ecj:JW0740 eco:b0757 |
PATRICi | fig|1411691.4.peg.1521 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02306 Genomic DNA Translation: CAA26172.1 U00096 Genomic DNA Translation: AAC73844.1 AP009048 Genomic DNA Translation: BAA35419.1 U13636 Genomic DNA Translation: AAB17019.1 |
PIRi | B23044, KIECGG |
RefSeqi | NP_415278.1, NC_000913.3 WP_000053415.1, NZ_SSZK01000002.1 |
3D structure databases
SMRi | P0A6T3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261711, 225 interactors 849734, 4 interactors |
IntActi | P0A6T3, 4 interactors |
STRINGi | 511145.b0757 |
Proteomic databases
jPOSTi | P0A6T3 |
PaxDbi | P0A6T3 |
PRIDEi | P0A6T3 |
Genome annotation databases
EnsemblBacteriai | AAC73844; AAC73844; b0757 BAA35419; BAA35419; BAA35419 |
GeneIDi | 945358 |
KEGGi | ecj:JW0740 eco:b0757 |
PATRICi | fig|1411691.4.peg.1521 |
Organism-specific databases
EchoBASEi | EB0358 |
Phylogenomic databases
eggNOGi | COG0153, Bacteria |
HOGENOMi | CLU_017814_2_1_6 |
InParanoidi | P0A6T3 |
PhylomeDBi | P0A6T3 |
Enzyme and pathway databases
UniPathwayi | UPA00214 |
BioCyci | EcoCyc:GALACTOKIN-MONOMER MetaCyc:GALACTOKIN-MONOMER |
SABIO-RKi | P0A6T3 |
Miscellaneous databases
PHI-basei | PHI:6269 |
PROi | PR:P0A6T3 |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit 3.30.70.890, 1 hit |
HAMAPi | MF_00246, Galactokinase, 1 hit |
InterProi | View protein in InterPro IPR000705, Galactokinase IPR022963, Galactokinase_bac IPR019741, Galactokinase_CS IPR019539, GalKase_N IPR013750, GHMP_kinase_C_dom IPR036554, GHMP_kinase_C_sf IPR006204, GHMP_kinase_N_dom IPR006203, GHMP_knse_ATP-bd_CS IPR006206, Mevalonate/galactokinase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr |
PANTHERi | PTHR10457:SF6, PTHR10457:SF6, 1 hit |
Pfami | View protein in Pfam PF10509, GalKase_gal_bdg, 1 hit PF08544, GHMP_kinases_C, 1 hit PF00288, GHMP_kinases_N, 1 hit |
PIRSFi | PIRSF000530, Galactokinase, 1 hit |
PRINTSi | PR00473, GALCTOKINASE |
SUPFAMi | SSF54211, SSF54211, 1 hit SSF55060, SSF55060, 1 hit |
TIGRFAMsi | TIGR00131, gal_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00106, GALACTOKINASE, 1 hit PS00627, GHMP_KINASES_ATP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GAL1_ECOLI | |
Accessioni | P0A6T3Primary (citable) accession number: P0A6T3 Secondary accession number(s): P06976 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 124 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families