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Entry version 132 (11 Dec 2019)
Sequence version 1 (10 May 2005)
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Protein

DNA-binding protein Fis

Gene

fis

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Activates ribosomal RNA transcription, as well other genes. Plays a direct role in upstream activation of rRNA promoters. Binds to a recombinational enhancer sequence that is required to stimulate hin-mediated DNA inversion. Prevents initiation of DNA replication from oriC. Binds to hundreds of transcriptionally active and inactive AT-rich sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed:16963779).3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section specifies the position and type of each DNA-binding domain present within the protein.<p><a href='/help/dna_bind' target='_top'>More...</a></p>DNA bindingi74 – 93H-T-H motifUniRule annotationAdd BLAST20

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:PD00196
ECOL316407:JW3229-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA-binding protein FisUniRule annotation
Alternative name(s):
Factor-for-inversion stimulation protein
Hin recombinational enhancer-binding protein
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:fisUniRule annotation
Ordered Locus Names:b3261, JW3229
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002038781 – 98DNA-binding protein FisAdd BLAST98

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
P0A6R3

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P0A6R3

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P0A6R3

PRoteomics IDEntifications database

More...
PRIDEi
P0A6R3

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

CollecTF database of bacterial transcription factor binding sites

More...
CollecTFi
EXPREG_00000770

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

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GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4261864, 136 interactors
852010, 1 interactor

Database of interacting proteins

More...
DIPi
DIP-47975N

Protein interaction database and analysis system

More...
IntActi
P0A6R3, 25 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b3261

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
P0A6R3

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P0A6R3

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni17 – 44Required for the stimulation of HIN-mediated recombinationAdd BLAST28

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the transcriptional regulatory Fis family.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG2901 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000256331

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P0A6R3

KEGG Orthology (KO)

More...
KOi
K03557

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P0A6R3

Family and domain databases

Database of protein disorder

More...
DisProti
DP00422

HAMAP database of protein families

More...
HAMAPi
MF_00166 DNA_binding_Fis, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR005412 Fis_DNA-bd
IPR009057 Homeobox-like_sf
IPR002197 HTH_Fis

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02954 HTH_8, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF002097 DNA-binding_Fis, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR01591 DNABINDNGFIS
PR01590 HTHFIS

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF46689 SSF46689, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0A6R3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFEQRVNSDV LTVSTVNSQD QVTQKPLRDS VKQALKNYFA QLNGQDVNDL
60 70 80 90
YELVLAEVEQ PLLDMVMQYT RGNQTRAALM MGINRGTLRK KLKKYGMN
Length:98
Mass (Da):11,240
Last modified:May 10, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i2E339BFCFCDB163C
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
J03245 Genomic DNA Translation: AAA83856.1
J03816 Genomic DNA Translation: AAA98812.1
M95784 Genomic DNA Translation: AAA23783.1
U18997 Genomic DNA Translation: AAA58065.1
U00096 Genomic DNA Translation: AAC76293.1
AP009048 Genomic DNA Translation: BAE77302.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A32142 DNECFS

NCBI Reference Sequences

More...
RefSeqi
NP_417727.1, NC_000913.3
WP_000462905.1, NZ_STEB01000012.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC76293; AAC76293; b3261
BAE77302; BAE77302; BAE77302

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
8911232
947697

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW3229
eco:b3261

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.3467

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03245 Genomic DNA Translation: AAA83856.1
J03816 Genomic DNA Translation: AAA98812.1
M95784 Genomic DNA Translation: AAA23783.1
U18997 Genomic DNA Translation: AAA58065.1
U00096 Genomic DNA Translation: AAC76293.1
AP009048 Genomic DNA Translation: BAE77302.1
PIRiA32142 DNECFS
RefSeqiNP_417727.1, NC_000913.3
WP_000462905.1, NZ_STEB01000012.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ETKX-ray2.10A/B1-98[»]
1ETOX-ray1.90A/B1-98[»]
1ETQX-ray2.80A/B/C/D1-98[»]
1ETVX-ray2.00A/B1-98[»]
1ETWX-ray2.00A/B1-98[»]
1ETXX-ray1.90A/B1-98[»]
1ETYX-ray2.00A/B1-98[»]
1F36X-ray2.65A/B1-98[»]
1FIAX-ray2.00A/B1-98[»]
1FIPX-ray1.90A/B1-98[»]
3FISX-ray2.30A/B1-98[»]
3IV5X-ray2.90A/B1-98[»]
3JR9X-ray2.90A/B1-98[»]
3JRAX-ray3.11A/B1-98[»]
3JRBX-ray3.10A/B1-98[»]
3JRCX-ray3.08A/B1-98[»]
3JRDX-ray3.10A/B1-98[»]
3JREX-ray3.17A/B1-98[»]
3JRFX-ray3.05A/B1-98[»]
3JRGX-ray3.11A/B1-98[»]
3JRHX-ray2.88A/B1-98[»]
3JRIX-ray3.11A/B1-98[»]
4FISX-ray2.30A/B1-98[»]
5DS9X-ray2.56A/B1-98[»]
5DTDX-ray2.64A/B1-98[»]
5E3LX-ray2.66A/B1-98[»]
5E3MX-ray2.89A/B1-98[»]
5E3NX-ray2.66A/B1-98[»]
5E3OX-ray2.78A/B1-98[»]
6P0SX-ray2.70A/B1-98[»]
6P0TX-ray3.60A/B1-98[»]
6P0UX-ray3.30A/B1-98[»]
SMRiP0A6R3
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi4261864, 136 interactors
852010, 1 interactor
DIPiDIP-47975N
IntActiP0A6R3, 25 interactors
STRINGi511145.b3261

Proteomic databases

EPDiP0A6R3
jPOSTiP0A6R3
PaxDbiP0A6R3
PRIDEiP0A6R3

Genome annotation databases

EnsemblBacteriaiAAC76293; AAC76293; b3261
BAE77302; BAE77302; BAE77302
GeneIDi8911232
947697
KEGGiecj:JW3229
eco:b3261
PATRICifig|1411691.4.peg.3467

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0313

Phylogenomic databases

eggNOGiCOG2901 LUCA
HOGENOMiHOG000256331
InParanoidiP0A6R3
KOiK03557
PhylomeDBiP0A6R3

Enzyme and pathway databases

BioCyciEcoCyc:PD00196
ECOL316407:JW3229-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0A6R3

Protein Ontology

More...
PROi
PR:P0A6R3

Gene expression databases

CollecTFiEXPREG_00000770

Family and domain databases

DisProtiDP00422
HAMAPiMF_00166 DNA_binding_Fis, 1 hit
InterProiView protein in InterPro
IPR005412 Fis_DNA-bd
IPR009057 Homeobox-like_sf
IPR002197 HTH_Fis
PfamiView protein in Pfam
PF02954 HTH_8, 1 hit
PIRSFiPIRSF002097 DNA-binding_Fis, 1 hit
PRINTSiPR01591 DNABINDNGFIS
PR01590 HTHFIS
SUPFAMiSSF46689 SSF46689, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiFIS_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0A6R3
Secondary accession number(s): P11028, P37404, Q2M8V4
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: December 11, 2019
This is version 132 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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