UniProtKB - P0A6M8 (EFG_ECOLI)
Protein
Elongation factor G
Gene
fusA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 17 – 24 | GTPBy similarity | 8 | |
Nucleotide bindingi | 88 – 92 | GTPBy similarity | 5 | |
Nucleotide bindingi | 142 – 145 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: InterPro
- GTP binding Source: UniProtKB-UniRule
- translation elongation factor activity Source: UniProtKB-UniRule
GO - Biological processi
- ribosome disassembly Source: GO_Central
Keywordsi
Molecular function | Elongation factor |
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10360-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor GShort name: EF-G |
Gene namesi | Name:fusA Synonyms:far, fus Ordered Locus Names:b3340, JW3302 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000091119 | 2 – 704 | Elongation factor GAdd BLAST | 703 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 504 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 643 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P0A6M8 |
PaxDbi | P0A6M8 |
PRIDEi | P0A6M8 |
2D gel databases
SWISS-2DPAGEi | P0A6M8 |
PTM databases
CarbonylDBi | P0A6M8 |
iPTMneti | P0A6M8 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259389, 142 interactors 852158, 1 interactor |
DIPi | DIP-31836N |
IntActi | P0A6M8, 44 interactors |
STRINGi | 511145.b3340 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A6M8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A6M8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 290 | tr-type GAdd BLAST | 283 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.Curated
Phylogenomic databases
eggNOGi | COG0480, Bacteria |
HOGENOMi | CLU_002794_4_1_6 |
InParanoidi | P0A6M8 |
PhylomeDBi | P0A6M8 |
Family and domain databases
CDDi | cd16262, EFG_III, 1 hit cd01434, EFG_mtEFG1_IV, 1 hit cd03713, EFG_mtEFG_C, 1 hit |
Gene3Di | 3.30.230.10, 1 hit |
HAMAPi | MF_00054_B, EF_G_EF_2_B, 1 hit |
InterProi | View protein in InterPro IPR041095, EFG_II IPR009022, EFG_III IPR035647, EFG_III/V IPR035649, EFG_V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR004540, Transl_elong_EFG/EF2 IPR005517, Transl_elong_EFG/EF2_IV |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF14492, EFG_III, 1 hit PF03764, EFG_IV, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SMARTi | View protein in SMART SM00838, EFG_C, 1 hit SM00889, EFG_IV, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR00484, EF-G, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A6M8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARTTPIARY RNIGISAHID AGKTTTTERI LFYTGVNHKI GEVHDGAATM
60 70 80 90 100
DWMEQEQERG ITITSAATTA FWSGMAKQYE PHRINIIDTP GHVDFTIEVE
110 120 130 140 150
RSMRVLDGAV MVYCAVGGVQ PQSETVWRQA NKYKVPRIAF VNKMDRMGAN
160 170 180 190 200
FLKVVNQIKT RLGANPVPLQ LAIGAEEHFT GVVDLVKMKA INWNDADQGV
210 220 230 240 250
TFEYEDIPAD MVELANEWHQ NLIESAAEAS EELMEKYLGG EELTEAEIKG
260 270 280 290 300
ALRQRVLNNE IILVTCGSAF KNKGVQAMLD AVIDYLPSPV DVPAINGILD
310 320 330 340 350
DGKDTPAERH ASDDEPFSAL AFKIATDPFV GNLTFFRVYS GVVNSGDTVL
360 370 380 390 400
NSVKAARERF GRIVQMHANK REEIKEVRAG DIAAAIGLKD VTTGDTLCDP
410 420 430 440 450
DAPIILERME FPEPVISIAV EPKTKADQEK MGLALGRLAK EDPSFRVWTD
460 470 480 490 500
EESNQTIIAG MGELHLDIIV DRMKREFNVE ANVGKPQVAY RETIRQKVTD
510 520 530 540 550
VEGKHAKQSG GRGQYGHVVI DMYPLEPGSN PKGYEFINDI KGGVIPGEYI
560 570 580 590 600
PAVDKGIQEQ LKAGPLAGYP VVDMGIRLHF GSYHDVDSSE LAFKLAASIA
610 620 630 640 650
FKEGFKKAKP VLLEPIMKVE VETPEENTGD VIGDLSRRRG MLKGQESEVT
660 670 680 690 700
GVKIHAEVPL SEMFGYATQL RSLTKGRASY TMEFLKYDEA PSNVAQAVIE
ARGK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 296 – 297 | NG → DC AA sequence (PubMed:7042386).Curated | 2 | |
Sequence conflicti | 300 – 302 | Missing AA sequence (PubMed:7042386).Curated | 3 | |
Sequence conflicti | 396 | T → C AA sequence (PubMed:7042386).Curated | 1 | |
Sequence conflicti | 576 | I → V AA sequence (PubMed:7042386).Curated | 1 | |
Sequence conflicti | 576 | I → V AA sequence (PubMed:7016587).Curated | 1 | |
Sequence conflicti | 584 | H → K AA sequence (PubMed:7042386).Curated | 1 | |
Sequence conflicti | 584 | H → K AA sequence (PubMed:7016587).Curated | 1 | |
Sequence conflicti | 594 | K → H AA sequence (PubMed:7042386).Curated | 1 | |
Sequence conflicti | 594 | K → H AA sequence (PubMed:7016587).Curated | 1 | |
Sequence conflicti | 626 | E → Q AA sequence (PubMed:7042386).Curated | 1 | |
Sequence conflicti | 626 | E → Q AA sequence (PubMed:7016587).Curated | 1 | |
Sequence conflicti | 646 | E → Q AA sequence (PubMed:7042386).Curated | 1 | |
Sequence conflicti | 646 | E → Q AA sequence (PubMed:7016587).Curated | 1 | |
Sequence conflicti | 657 | E → Q AA sequence (PubMed:7042386).Curated | 1 | |
Sequence conflicti | 657 | E → Q AA sequence (PubMed:7016587).Curated | 1 | |
Sequence conflicti | 662 | E → EQ AA sequence (PubMed:7042386).Curated | 1 | |
Sequence conflicti | 662 | E → EQ AA sequence (PubMed:7016587).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00415 Genomic DNA Translation: CAA25120.1 X64592 Genomic DNA No translation available. U18997 Genomic DNA Translation: AAA58137.1 U00096 Genomic DNA Translation: AAC76365.1 AP009048 Genomic DNA Translation: BAE77951.1 J01689 Genomic DNA Translation: AAA50991.1 X65735 Genomic DNA Translation: CAA46645.1 |
PIRi | G65127, EFECG |
RefSeqi | NP_417799.1, NC_000913.3 WP_000124700.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76365; AAC76365; b3340 BAE77951; BAE77951; BAE77951 |
GeneIDi | 58348822 947847 |
KEGGi | ecj:JW3302 eco:b3340 |
PATRICi | fig|1411691.4.peg.3391 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00415 Genomic DNA Translation: CAA25120.1 X64592 Genomic DNA No translation available. U18997 Genomic DNA Translation: AAA58137.1 U00096 Genomic DNA Translation: AAC76365.1 AP009048 Genomic DNA Translation: BAE77951.1 J01689 Genomic DNA Translation: AAA50991.1 X65735 Genomic DNA Translation: CAA46645.1 |
PIRi | G65127, EFECG |
RefSeqi | NP_417799.1, NC_000913.3 WP_000124700.1, NZ_STEB01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2RDO | electron microscopy | 9.10 | 7 | 1-704 | [»] | |
3J0E | electron microscopy | 9.90 | H | 2-703 | [»] | |
3J9Z | electron microscopy | 3.60 | S1 | 2-703 | [»] | |
3JA1 | electron microscopy | 3.60 | S3 | 2-703 | [»] | |
4V7B | electron microscopy | 6.80 | AY | 1-704 | [»] | |
4V7D | electron microscopy | 7.60 | BZ | 2-704 | [»] | |
4V9O | X-ray | 2.90 | BV/DV/FV/HV | 1-704 | [»] | |
4V9P | X-ray | 2.90 | BV/DV/FV/HV | 1-704 | [»] | |
SMRi | P0A6M8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259389, 142 interactors 852158, 1 interactor |
DIPi | DIP-31836N |
IntActi | P0A6M8, 44 interactors |
STRINGi | 511145.b3340 |
PTM databases
CarbonylDBi | P0A6M8 |
iPTMneti | P0A6M8 |
2D gel databases
SWISS-2DPAGEi | P0A6M8 |
Proteomic databases
jPOSTi | P0A6M8 |
PaxDbi | P0A6M8 |
PRIDEi | P0A6M8 |
Genome annotation databases
EnsemblBacteriai | AAC76365; AAC76365; b3340 BAE77951; BAE77951; BAE77951 |
GeneIDi | 58348822 947847 |
KEGGi | ecj:JW3302 eco:b3340 |
PATRICi | fig|1411691.4.peg.3391 |
Organism-specific databases
EchoBASEi | EB0355 |
Phylogenomic databases
eggNOGi | COG0480, Bacteria |
HOGENOMi | CLU_002794_4_1_6 |
InParanoidi | P0A6M8 |
PhylomeDBi | P0A6M8 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10360-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A6M8 |
PROi | PR:P0A6M8 |
Family and domain databases
CDDi | cd16262, EFG_III, 1 hit cd01434, EFG_mtEFG1_IV, 1 hit cd03713, EFG_mtEFG_C, 1 hit |
Gene3Di | 3.30.230.10, 1 hit |
HAMAPi | MF_00054_B, EF_G_EF_2_B, 1 hit |
InterProi | View protein in InterPro IPR041095, EFG_II IPR009022, EFG_III IPR035647, EFG_III/V IPR035649, EFG_V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR004540, Transl_elong_EFG/EF2 IPR005517, Transl_elong_EFG/EF2_IV |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF14492, EFG_III, 1 hit PF03764, EFG_IV, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SMARTi | View protein in SMART SM00838, EFG_C, 1 hit SM00889, EFG_IV, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR00484, EF-G, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EFG_ECOLI | |
Accessioni | P0A6M8Primary (citable) accession number: P0A6M8 Secondary accession number(s): P02996, Q2M705, Q9F439 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families