UniProtKB - P0A6M2 (DSBB_ECOLI)
Protein
Disulfide bond formation protein B
Gene
dsbB
Organism
Escherichia coli (strain K12)
Status
Functioni
Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by oxidizing the DsbA protein. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.3 Publications
GO - Molecular functioni
- electron transfer activity Source: UniProtKB-UniRule
- oxidoreductase activity, acting on a sulfur group of donors, quinone or similar compound as acceptor Source: EcoCyc
- protein disulfide oxidoreductase activity Source: EcoCyc
- ubiquinone binding Source: EcoCyc
GO - Biological processi
- protein folding Source: GO_Central
- response to heat Source: EcoCyc
Keywordsi
Molecular function | Chaperone, Oxidoreductase |
Biological process | Electron transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:DSBBPROT-MONOMER MetaCyc:DSBBPROT-MONOMER |
Protein family/group databases
TCDBi | 5.A.2.1.1, the disulfide bond oxidoreductase b (dsbb) family |
Names & Taxonomyi
Protein namesi | Recommended name: Disulfide bond formation protein BAlternative name(s): Disulfide oxidoreductase |
Gene namesi | Name:dsbB Synonyms:roxB, ycgA Ordered Locus Names:b1185, JW5182 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 2 Publications; Multi-pass membrane protein 2 Publications
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 14 | CytoplasmicCuratedAdd BLAST | 14 | |
Transmembranei | 15 – 31 | HelicalCuratedAdd BLAST | 17 | |
Topological domaini | 32 – 49 | PeriplasmicCuratedAdd BLAST | 18 | |
Transmembranei | 50 – 65 | HelicalCuratedAdd BLAST | 16 | |
Topological domaini | 66 – 71 | CytoplasmicCurated | 6 | |
Transmembranei | 72 – 89 | HelicalCuratedAdd BLAST | 18 | |
Topological domaini | 90 – 144 | PeriplasmicCuratedAdd BLAST | 55 | |
Transmembranei | 145 – 163 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 164 – 176 | CytoplasmicCuratedAdd BLAST | 13 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Induction of the PhoP/PhoQ two-component regulatory system.1 Publication
Chemistry databases
DrugBanki | DB08689, Ubiquinone Q1 DB08690, Ubiquinone Q2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059342 | 1 – 176 | Disulfide bond formation protein BAdd BLAST | 176 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 41 ↔ 44 | Redox-active1 Publication | ||
Disulfide bondi | 104 ↔ 130 | Redox-active1 Publication |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P0A6M2 |
PaxDbi | P0A6M2 |
PRIDEi | P0A6M2 |
Interactioni
Binary interactionsi
P0A6M2
With | #Exp. | IntAct |
---|---|---|
dsbA [P0AEG4] | 5 | EBI-1170740,EBI-549711 |
Protein-protein interaction databases
BioGRIDi | 4261815, 428 interactors |
IntActi | P0A6M2, 1 interactor |
MINTi | P0A6M2 |
STRINGi | 511145.b1185 |
Chemistry databases
BindingDBi | P0A6M2 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0A6M2 |
SMRi | P0A6M2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A6M2 |
Family & Domainsi
Sequence similaritiesi
Belongs to the DsbB family.Curated
Keywords - Domaini
Redox-active center, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1495, Bacteria |
HOGENOMi | CLU_098660_2_0_6 |
InParanoidi | P0A6M2 |
Family and domain databases
Gene3Di | 1.20.1550.10, 1 hit |
HAMAPi | MF_00286, DsbB, 1 hit |
InterProi | View protein in InterPro IPR003752, DiS_bond_form_DsbB/BdbC IPR022920, Disulphide_bond_form_DsbB IPR023380, DsbB-like_sf |
Pfami | View protein in Pfam PF02600, DsbB, 1 hit |
SUPFAMi | SSF158442, SSF158442, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A6M2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRFLNQCSQ GRGAWLLMAF TALALELTAL WFQHVMLLKP CVLCIYERCA
60 70 80 90 100
LFGVLGAALI GAIAPKTPLR YVAMVIWLYS AFRGVQLTYE HTMLQLYPSP
110 120 130 140 150
FATCDFMVRF PEWLPLDKWV PQVFVASGDC AERQWDFLGL EMPQWLLGIF
160 170
IAYLIVAVLV VISQPFKAKK RDLFGR
Sequence cautioni
The sequence AAA23711 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAA24220 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L03721 Genomic DNA Translation: AAA23711.1 Different initiation. U00096 Genomic DNA Translation: AAC74269.1 AP009048 Genomic DNA Translation: BAA36032.2 M83655 Genomic DNA Translation: AAA24220.1 Frameshift. |
PIRi | F64864 |
RefSeqi | NP_415703.3, NC_000913.3 WP_000943459.1, NZ_SSZK01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC74269; AAC74269; b1185 BAA36032; BAA36032; BAA36032 |
GeneIDi | 48725909 946344 |
KEGGi | ecj:JW5182 eco:b1185 |
PATRICi | fig|1411691.4.peg.1102 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L03721 Genomic DNA Translation: AAA23711.1 Different initiation. U00096 Genomic DNA Translation: AAC74269.1 AP009048 Genomic DNA Translation: BAA36032.2 M83655 Genomic DNA Translation: AAA24220.1 Frameshift. |
PIRi | F64864 |
RefSeqi | NP_415703.3, NC_000913.3 WP_000943459.1, NZ_SSZK01000010.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2HI7 | X-ray | 3.70 | B | 1-176 | [»] | |
2K73 | NMR | - | A | 1-176 | [»] | |
2K74 | NMR | - | A | 1-176 | [»] | |
2LEG | NMR | - | B | 1-176 | [»] | |
2LTQ | NMR | - | A/D | 1-176 | [»] | |
2ZUP | X-ray | 3.70 | B | 1-176 | [»] | |
2ZUQ | X-ray | 3.30 | A/D | 1-176 | [»] | |
3E9J | X-ray | 3.70 | C/F | 1-176 | [»] | |
BMRBi | P0A6M2 | |||||
SMRi | P0A6M2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261815, 428 interactors |
IntActi | P0A6M2, 1 interactor |
MINTi | P0A6M2 |
STRINGi | 511145.b1185 |
Chemistry databases
BindingDBi | P0A6M2 |
DrugBanki | DB08689, Ubiquinone Q1 DB08690, Ubiquinone Q2 |
Protein family/group databases
TCDBi | 5.A.2.1.1, the disulfide bond oxidoreductase b (dsbb) family |
Proteomic databases
jPOSTi | P0A6M2 |
PaxDbi | P0A6M2 |
PRIDEi | P0A6M2 |
Protocols and materials databases
ABCDi | P0A6M2, 1 sequenced antibody |
Genome annotation databases
EnsemblBacteriai | AAC74269; AAC74269; b1185 BAA36032; BAA36032; BAA36032 |
GeneIDi | 48725909 946344 |
KEGGi | ecj:JW5182 eco:b1185 |
PATRICi | fig|1411691.4.peg.1102 |
Organism-specific databases
EchoBASEi | EB1366 |
Phylogenomic databases
eggNOGi | COG1495, Bacteria |
HOGENOMi | CLU_098660_2_0_6 |
InParanoidi | P0A6M2 |
Enzyme and pathway databases
BioCyci | EcoCyc:DSBBPROT-MONOMER MetaCyc:DSBBPROT-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A6M2 |
PROi | PR:P0A6M2 |
Family and domain databases
Gene3Di | 1.20.1550.10, 1 hit |
HAMAPi | MF_00286, DsbB, 1 hit |
InterProi | View protein in InterPro IPR003752, DiS_bond_form_DsbB/BdbC IPR022920, Disulphide_bond_form_DsbB IPR023380, DsbB-like_sf |
Pfami | View protein in Pfam PF02600, DsbB, 1 hit |
SUPFAMi | SSF158442, SSF158442, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DSBB_ECOLI | |
Accessioni | P0A6M2Primary (citable) accession number: P0A6M2 Secondary accession number(s): P30018, Q47408 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | May 10, 2005 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families