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UniProtKB - P0A6M2 (DSBB_ECOLI)

Protein

Disulfide bond formation protein B

Gene

dsbB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by oxidizing the DsbA protein. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.3 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • electron transfer activity Source: UniProtKB-UniRule
  • protein disulfide oxidoreductase activity Source: EcoCyc
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • electron transport chain Source: EcoCyc
  • protein folding Source: GO_Central
  • response to heat Source: EcoCyc
View the complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionChaperone, Oxidoreductase
Biological processElectron transport, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:DSBBPROT-MONOMER
MetaCyc:DSBBPROT-MONOMER

Protein family/group databases

Transport Classification Database

More...TCDBi		5.A.2.1.1 the disulfide bond oxidoreductase b (dsbb) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Disulfide bond formation protein B
Alternative name(s):
Disulfide oxidoreductase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:dsbB
Synonyms:roxB, ycgA
Ordered Locus Names:b1185, JW5182
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...EcoGenei		EG11393 dsbB

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 14CytoplasmicCuratedAdd BLAST14
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei15 – 31HelicalCuratedAdd BLAST17
Topological domaini32 – 49PeriplasmicCuratedAdd BLAST18
Transmembranei50 – 65HelicalCuratedAdd BLAST16
Topological domaini66 – 71CytoplasmicCurated6
Transmembranei72 – 89HelicalCuratedAdd BLAST18
Topological domaini90 – 144PeriplasmicCuratedAdd BLAST55
Transmembranei145 – 163HelicalCuratedAdd BLAST19
Topological domaini164 – 176CytoplasmicCuratedAdd BLAST13

GO - Cellular componenti

View the complete GO annotation on QuickGO ...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Induction of the PhoP/PhoQ two-component regulatory system.1 Publication

Chemistry databases

Drug and drug target database

More...DrugBanki		DB08689 UBIQUINONE-1
DB08690 UBIQUINONE-2

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000593421 – 176Disulfide bond formation protein BAdd BLAST176

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the PTM / Processing":/help/ptm_processing_section section describes the positions of cysteine residues participating in disulfide bonds.<p><a href='/help/disulfid' target='_top'>More...</a></p>Disulfide bondi41 ↔ 44Redox-active1 Publication
Disulfide bondi104 ↔ 130Redox-active1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P0A6M2

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P0A6M2

PRoteomics IDEntifications database

More...PRIDEi		P0A6M2

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
dsbAP0AEG45EBI-1170740,EBI-549711

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...BioGridi		4261815, 428 interactors

Protein interaction database and analysis system

More...IntActi		P0A6M2, 1 interactor

Molecular INTeraction database

More...MINTi		P0A6M2

STRING: functional protein association networks

More...STRINGi		316385.ECDH10B_1238

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		P0A6M2

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HI7X-ray3.70B1-176[»]
2K73NMR-A1-176[»]
2K74NMR-A1-176[»]
2LEGNMR-B1-176[»]
2LTQNMR-A/D1-176[»]
2ZUPX-ray3.70B1-176[»]
2ZUQX-ray3.30A/D1-176[»]
3E9JX-ray3.70C/F1-176[»]

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...ProteinModelPortali		P0A6M2

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0A6M2

Database of comparative protein structure models

More...ModBasei		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P0A6M2

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the DsbB family.Curated

Keywords - Domaini

Redox-active center, Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG410635M Bacteria
COG1495 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		HOG000218135

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P0A6M2

KEGG Orthology (KO)

More...KOi		K03611

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.20.1550.10, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_00286 DsbB, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR003752 DiS_bond_form_DsbB/BdbC
IPR022920 Disulphide_bond_form_DsbB
IPR023380 DsbB-like_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF02600 DsbB, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF158442 SSF158442, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0A6M2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRFLNQCSQ GRGAWLLMAF TALALELTAL WFQHVMLLKP CVLCIYERCA 
        60         70         80         90        100
LFGVLGAALI GAIAPKTPLR YVAMVIWLYS AFRGVQLTYE HTMLQLYPSP 
       110        120        130        140        150
FATCDFMVRF PEWLPLDKWV PQVFVASGDC AERQWDFLGL EMPQWLLGIF 
       160        170 
IAYLIVAVLV VISQPFKAKK RDLFGR
Length:176
Mass (Da):20,142
Last modified:May 10, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9CBD673D51E9F09B
GO

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA23711 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAA24220 differs from that shown. Reason: Frameshift at position 130.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
L03721 Genomic DNA Translation: AAA23711.1 Different initiation.
U00096 Genomic DNA Translation: AAC74269.1
AP009048 Genomic DNA Translation: BAA36032.2
M83655 Genomic DNA Translation: AAA24220.1 Frameshift.

Protein sequence database of the Protein Information Resource

More...PIRi		F64864

NCBI Reference Sequences

More...RefSeqi		NP_415703.3, NC_000913.3
WP_000943459.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC74269; AAC74269; b1185
BAA36032; BAA36032; BAA36032

Database of genes from NCBI RefSeq genomes

More...GeneIDi		946344

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW5182
eco:b1185

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.1102

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03721 Genomic DNA Translation: AAA23711.1 Different initiation.
U00096 Genomic DNA Translation: AAC74269.1
AP009048 Genomic DNA Translation: BAA36032.2
M83655 Genomic DNA Translation: AAA24220.1 Frameshift.
PIRiF64864
RefSeqiNP_415703.3, NC_000913.3
WP_000943459.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HI7X-ray3.70B1-176[»]
2K73NMR-A1-176[»]
2K74NMR-A1-176[»]
2LEGNMR-B1-176[»]
2LTQNMR-A/D1-176[»]
2ZUPX-ray3.70B1-176[»]
2ZUQX-ray3.30A/D1-176[»]
3E9JX-ray3.70C/F1-176[»]
ProteinModelPortaliP0A6M2
SMRiP0A6M2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261815, 428 interactors
IntActiP0A6M2, 1 interactor
MINTiP0A6M2
STRINGi316385.ECDH10B_1238

Chemistry databases

BindingDBiP0A6M2
DrugBankiDB08689 UBIQUINONE-1
DB08690 UBIQUINONE-2

Protein family/group databases

TCDBi5.A.2.1.1 the disulfide bond oxidoreductase b (dsbb) family

Proteomic databases

jPOSTiP0A6M2
PaxDbiP0A6M2
PRIDEiP0A6M2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74269; AAC74269; b1185
BAA36032; BAA36032; BAA36032
GeneIDi946344
KEGGiecj:JW5182
eco:b1185
PATRICifig|1411691.4.peg.1102

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB1366
EcoGeneiEG11393 dsbB

Phylogenomic databases

eggNOGiENOG410635M Bacteria
COG1495 LUCA
HOGENOMiHOG000218135
InParanoidiP0A6M2
KOiK03611

Enzyme and pathway databases

BioCyciEcoCyc:DSBBPROT-MONOMER
MetaCyc:DSBBPROT-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0A6M2

Protein Ontology

More...PROi		PR:P0A6M2

Family and domain databases

Gene3Di1.20.1550.10, 1 hit
HAMAPiMF_00286 DsbB, 1 hit
InterProiView protein in InterPro
IPR003752 DiS_bond_form_DsbB/BdbC
IPR022920 Disulphide_bond_form_DsbB
IPR023380 DsbB-like_sf
PfamiView protein in Pfam
PF02600 DsbB, 1 hit
SUPFAMiSSF158442 SSF158442, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDSBB_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0A6M2
Secondary accession number(s): P30018, Q47408
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: January 16, 2019
This is version 111 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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