UniProtKB - P0A6M2 (DSBB_ECOLI)

BLAST

>sp|P0A6M2|DSBB_ECOLI Disulfide bond formation protein B OS=Escherichia coli (strain K12) OX=83333 GN=dsbB PE=1 SV=1
MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLCIYERCALFGVLGAALI
GAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWV
PQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLVVISQPFKAKKRDLFGR

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History
Entry version 110 (07 Nov 2018)
Sequence version 1 (10 May 2005)
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Protein

Disulfide bond formation protein B

Gene

dsbB

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by oxidizing the DsbA protein. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.3 Publications

GO - Molecular functionⁱ

electron transfer activity Source: UniProtKB-UniRule
protein disulfide oxidoreductase activity
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 8430071

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

electron transport chain
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 12006488
protein folding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
response to heat
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 24580753

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Chaperone, Oxidoreductase
Biological process	Electron transport, Transport

Enzyme and pathway databases

BioCycⁱ	EcoCyc:DSBBPROT-MONOMER MetaCyc:DSBBPROT-MONOMER

BioCycⁱ

EcoCyc:DSBBPROT-MONOMER
MetaCyc:DSBBPROT-MONOMER

Protein family/group databases

TCDBⁱ	5.A.2.1.1 the disulfide bond oxidoreductase b (dsbb) family

TCDBⁱ

5.A.2.1.1 the disulfide bond oxidoreductase b (dsbb) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Disulfide bond formation protein B Alternative name(s): Disulfide oxidoreductase
Gene namesⁱ	Name:dsbB Synonyms:roxB, ycgA Ordered Locus Names:b1185, JW5182
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11393 dsbB

EcoGeneⁱ

EG11393 dsbB

Subcellular locationⁱ

Cell inner membrane
2 Publications
Manual assertion based on experiment inⁱ
- Ref.1
  "A pathway for disulfide bond formation in vivo."
  Bardwell J.C.A., Lee J.-O., Jander G., Martin N., Belin D., Beckwith J.
  Proc. Natl. Acad. Sci. U.S.A. 90:1038-1042(1993) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
- Ref.7
  "Two cysteines in each periplasmic domain of the membrane protein DsbB are required for its function in protein disulfide bond formation."
  Jander G., Martin N.L., Beckwith J.
  EMBO J. 13:5121-5127(1994) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF CYSTEINE RESIDUES.
; Multi-pass membrane protein
2 Publications
Manual assertion based on experiment inⁱ
- Ref.1
  "A pathway for disulfide bond formation in vivo."
  Bardwell J.C.A., Lee J.-O., Jander G., Martin N., Belin D., Beckwith J.
  Proc. Natl. Acad. Sci. U.S.A. 90:1038-1042(1993) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
- Ref.7
  "Two cysteines in each periplasmic domain of the membrane protein DsbB are required for its function in protein disulfide bond formation."
  Jander G., Martin N.L., Beckwith J.
  EMBO J. 13:5121-5127(1994) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF CYSTEINE RESIDUES.

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 14	CytoplasmicCuratedAdd BLAST	14
Transmembraneⁱ	15 – 31	HelicalCuratedAdd BLAST	17
Topological domainⁱ	32 – 49	PeriplasmicCuratedAdd BLAST	18
Transmembraneⁱ	50 – 65	HelicalCuratedAdd BLAST	16
Topological domainⁱ	66 – 71	CytoplasmicCurated	6
Transmembraneⁱ	72 – 89	HelicalCuratedAdd BLAST	18
Topological domainⁱ	90 – 144	PeriplasmicCuratedAdd BLAST	55
Transmembraneⁱ	145 – 163	HelicalCuratedAdd BLAST	19
Topological domainⁱ	164 – 176	CytoplasmicCuratedAdd BLAST	13

GO - Cellular componentⁱ

integral component of membrane Source: UniProtKB-KW
plasma membrane Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Induction of the PhoP/PhoQ two-component regulatory system.1 Publication

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB08689 UBIQUINONE-1 DB08690 UBIQUINONE-2

DrugBankⁱ

DB08689 UBIQUINONE-1
DB08690 UBIQUINONE-2

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000059342	1 – 176	Disulfide bond formation protein BAdd BLAST		176

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Disulfide bondⁱ	41 ↔ 44	Redox-active1 Publication
Disulfide bondⁱ	104 ↔ 130	Redox-active1 Publication

Keywords - PTMⁱ

Disulfide bond

Proteomic databases

PaxDbⁱ	P0A6M2
PRIDEⁱ	P0A6M2

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
dsbA	P0AEG4	5	EBI-1170740,EBI-549711

With

Entry

#Exp.

IntAct

Notes

dsbA

P0AEG4

EBI-1170740,EBI-549711

Protein-protein interaction databases

BioGridⁱ	4261815, 428 interactors
IntActⁱ	P0A6M2, 1 interactor
Molecular INTeraction database More...MINTⁱ	P0A6M2
STRINGⁱ	316385.ECDH10B_1238

Chemistry databases

BindingDBⁱ

P0A6M2

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	2 – 9	Combined sources	8
Helixⁱ	13 – 21	Combined sources	9
Helixⁱ	23 – 34	Combined sources	12
Helixⁱ	44 – 62	Combined sources	19
Beta strandⁱ	66 – 69	Combined sources	4
Helixⁱ	70 – 96	Combined sources	27
Helixⁱ	115 – 118	Combined sources	4
Turnⁱ	122 – 124	Combined sources	3
Beta strandⁱ	138 – 140	Combined sources	3
Helixⁱ	142 – 157	Combined sources	16
Turnⁱ	158 – 161	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	P0A6M2
SMRⁱ	P0A6M2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P0A6M2

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the DsbB family.Curated

Keywords - Domainⁱ

Redox-active center, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410635M Bacteria COG1495 LUCA
HOGENOMⁱ	HOG000218135
InParanoidⁱ	P0A6M2
KEGG Orthology (KO) More...KOⁱ	K03611

Family and domain databases

Gene3Dⁱ	1.20.1550.10, 1 hit
HAMAPⁱ	MF_00286 DsbB, 1 hit
InterProⁱ	View protein in InterPro IPR003752 DiS_bond_form_DsbB/BdbC IPR022920 Disulphide_bond_form_DsbB IPR023380 DsbB-like_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02600 DsbB, 1 hit
SUPFAMⁱ	SSF158442 SSF158442, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0A6M2-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLRFLNQCSQ GRGAWLLMAF TALALELTAL WFQHVMLLKP CVLCIYERCA 
        60         70         80         90        100
LFGVLGAALI GAIAPKTPLR YVAMVIWLYS AFRGVQLTYE HTMLQLYPSP 
       110        120        130        140        150
FATCDFMVRF PEWLPLDKWV PQVFVASGDC AERQWDFLGL EMPQWLLGIF 
       160        170 
IAYLIVAVLV VISQPFKAKK RDLFGR

Length:176

Mass (Da):20,142

Last modified:May 10, 2005 - v1

Checksum:ⁱ9CBD673D51E9F09B

Sequence cautionⁱ

The sequence AAA23711 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

The sequence AAA24220 differs from that shown. Reason: Frameshift at position 130.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L03721 Genomic DNA Translation: AAA23711.1 Different initiation. U00096 Genomic DNA Translation: AAC74269.1 AP009048 Genomic DNA Translation: BAA36032.2 M83655 Genomic DNA Translation: AAA24220.1 Frameshift.
PIRⁱ	F64864
NCBI Reference Sequences More...RefSeqⁱ	NP_415703.3, NC_000913.3 WP_000943459.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC74269; AAC74269; b1185 BAA36032; BAA36032; BAA36032
GeneIDⁱ	946344
KEGGⁱ	ecj:JW5182 eco:b1185
PATRICⁱ	fig\|1411691.4.peg.1102

Protein	Similar proteins		Species	Score	Length	Source
P0A6M2	Disulfide bond formation protein B		ECO57		176	UniRef100_P0A6M3
Disulfide bond formation protein B		SHIDS		176
Disulfide bond formation protein B		ECOLX		178
Disulfide bond formation protein B		Escherichia coli 907672		178
Disulfide bond formation protein B		Escherichia coli 908525		178
+94

Protein	Similar proteins		Species	Score	Length	Source
P0A6M2	Disulfide bond formation protein B		ECOK1		176	UniRef90_A1AAA8
Disulfide bond formation protein B		ECOL6		176
Disulfide bond formation protein B		ECOUT		176
Disulfide bond formation protein B		ECO57		176
Disulfide bond formation protein B		SHIDS		176
+353

Protein	Similar proteins		Species	Score	Length	Source
P0A6M2	Disulfide bond formation protein B		YERPA		176	UniRef50_Q1C7V4
Disulfide bond formation protein B		YERPE		176
Disulfide bond formation protein B		YERPN		176
Disulfide bond formation protein B		YERPP		176
Disulfide bond formation protein B		YERPS		176
+844

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L03721 Genomic DNA Translation: AAA23711.1 Different initiation. U00096 Genomic DNA Translation: AAC74269.1 AP009048 Genomic DNA Translation: BAA36032.2 M83655 Genomic DNA Translation: AAA24220.1 Frameshift.
PIRⁱ	F64864
RefSeqⁱ	NP_415703.3, NC_000913.3 WP_000943459.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2HI7	X-ray	3.70	B	1-176	[»]
	2K73	NMR	-	A	1-176	[»]
	2K74	NMR	-	A	1-176	[»]
	2LEG	NMR	-	B	1-176	[»]
	2LTQ	NMR	-	A/D	1-176	[»]
	2ZUP	X-ray	3.70	B	1-176	[»]
	2ZUQ	X-ray	3.30	A/D	1-176	[»]
	3E9J	X-ray	3.70	C/F	1-176	[»]
ProteinModelPortalⁱ	P0A6M2
SMRⁱ	P0A6M2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261815, 428 interactors
IntActⁱ	P0A6M2, 1 interactor
MINTⁱ	P0A6M2
STRINGⁱ	316385.ECDH10B_1238

Chemistry databases

BindingDBⁱ	P0A6M2
DrugBankⁱ	DB08689 UBIQUINONE-1 DB08690 UBIQUINONE-2

Protein family/group databases

TCDBⁱ	5.A.2.1.1 the disulfide bond oxidoreductase b (dsbb) family

TCDBⁱ

5.A.2.1.1 the disulfide bond oxidoreductase b (dsbb) family

Proteomic databases

PaxDbⁱ	P0A6M2
PRIDEⁱ	P0A6M2

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74269; AAC74269; b1185 BAA36032; BAA36032; BAA36032
GeneIDⁱ	946344
KEGGⁱ	ecj:JW5182 eco:b1185
PATRICⁱ	fig\|1411691.4.peg.1102

Organism-specific databases

EchoBASEⁱ	EB1366
EcoGeneⁱ	EG11393 dsbB

Phylogenomic databases

eggNOGⁱ	ENOG410635M Bacteria COG1495 LUCA
HOGENOMⁱ	HOG000218135
InParanoidⁱ	P0A6M2
KOⁱ	K03611

Enzyme and pathway databases

BioCycⁱ	EcoCyc:DSBBPROT-MONOMER MetaCyc:DSBBPROT-MONOMER

BioCycⁱ

EcoCyc:DSBBPROT-MONOMER
MetaCyc:DSBBPROT-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P0A6M2
Protein Ontology More...PROⁱ	PR:P0A6M2

Family and domain databases

Gene3Dⁱ	1.20.1550.10, 1 hit
HAMAPⁱ	MF_00286 DsbB, 1 hit
InterProⁱ	View protein in InterPro IPR003752 DiS_bond_form_DsbB/BdbC IPR022920 Disulphide_bond_form_DsbB IPR023380 DsbB-like_sf
Pfamⁱ	View protein in Pfam PF02600 DsbB, 1 hit
SUPFAMⁱ	SSF158442 SSF158442, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DSBB_ECOLI
Accessionⁱ	P0A6M2Primary (citable) accession number: P0A6M2 Secondary accession number(s): P30018, Q47408
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
	Last sequence update:	May 10, 2005
	Last modified:	November 7, 2018
	This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0A6M2 (DSBB_ECOLI)

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Disulfide bond formation protein B

dsbB

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ