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Protein

D-alanine--D-alanine ligase A

Gene

ddlA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi302Magnesium or manganese 1By similarity1
Metal bindingi315Magnesium or manganese 1By similarity1
Metal bindingi315Magnesium or manganese 2By similarity1
Metal bindingi317Magnesium or manganese 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi175 – 230ATPBy similarityAdd BLAST56

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • D-alanine-D-alanine ligase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: GO_Central
  • regulation of cell shape Source: UniProtKB-KW
  • response to X-ray Source: EcoCyc

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:DALADALALIGA-MONOMER
MetaCyc:DALADALALIGA-MONOMER
UniPathwayi
UPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligase A (EC:6.3.2.4)
Alternative name(s):
D-Ala-D-Ala ligase A
D-alanylalanine synthetase A
Gene namesi
Name:ddlA
Ordered Locus Names:b0381, JW0372
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10213 ddlA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB00260 Cycloserine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001778151 – 364D-alanine--D-alanine ligase AAdd BLAST364

Proteomic databases

EPDiP0A6J8
PaxDbiP0A6J8
PRIDEiP0A6J8

Interactioni

Protein-protein interaction databases

BioGridi4261531, 464 interactors
DIPiDIP-47939N
IntActiP0A6J8, 14 interactors
STRINGi316385.ECDH10B_0338

Structurei

3D structure databases

ProteinModelPortaliP0A6J8
SMRiP0A6J8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini145 – 348ATP-graspAdd BLAST204

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.Curated

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011593
InParanoidiP0A6J8
KOiK01921
PhylomeDBiP0A6J8

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0A6J8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKLRVGIVF GGKSAEHEVS LQSAKNIVDA IDKSRFDVVL LGIDKQGQWH
60 70 80 90 100
VSDASNYLLN ADDPAHIALR PSATSLAQVP GKHEHQLIDA QNGQPLPTVD
110 120 130 140 150
VIFPIVHGTL GEDGSLQGML RVANLPFVGS DVLASAACMD KDVTKRLLRD
160 170 180 190 200
AGLNIAPFIT LTRANRHNIS FAEVESKLGL PLFVKPANQG SSVGVSKVTS
210 220 230 240 250
EEQYAIAVDL AFEFDHKVIV EQGIKGREIE CAVLGNDNPQ ASTCGEIVLT
260 270 280 290 300
SDFYAYDTKY IDEDGAKVVV PAAIAPEIND KIRAIAVQAY QTLGCAGMAR
310 320 330 340 350
VDVFLTPENE VVINEINTLP GFTNISMYPK LWQASGLGYT DLITRLIELA
360
LERHAADNAL KTTM
Length:364
Mass (Da):39,316
Last modified:March 29, 2005 - v1
Checksum:iC7FDBEC353AC1320
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58467 Genomic DNA Translation: AAA23671.1
U73857 Genomic DNA Translation: AAB18104.1
U00096 Genomic DNA Translation: AAC73484.1
AP009048 Genomic DNA Translation: BAE76162.1
PIRiA39182 CEECDA
RefSeqiNP_414915.1, NC_000913.3
WP_000413677.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73484; AAC73484; b0381
BAE76162; BAE76162; BAE76162
GeneIDi945313
KEGGiecj:JW0372
eco:b0381
PATRICifig|1411691.4.peg.1897

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58467 Genomic DNA Translation: AAA23671.1
U73857 Genomic DNA Translation: AAB18104.1
U00096 Genomic DNA Translation: AAC73484.1
AP009048 Genomic DNA Translation: BAE76162.1
PIRiA39182 CEECDA
RefSeqiNP_414915.1, NC_000913.3
WP_000413677.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP0A6J8
SMRiP0A6J8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261531, 464 interactors
DIPiDIP-47939N
IntActiP0A6J8, 14 interactors
STRINGi316385.ECDH10B_0338

Chemistry databases

DrugBankiDB00260 Cycloserine

Proteomic databases

EPDiP0A6J8
PaxDbiP0A6J8
PRIDEiP0A6J8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73484; AAC73484; b0381
BAE76162; BAE76162; BAE76162
GeneIDi945313
KEGGiecj:JW0372
eco:b0381
PATRICifig|1411691.4.peg.1897

Organism-specific databases

EchoBASEiEB0209
EcoGeneiEG10213 ddlA

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011593
InParanoidiP0A6J8
KOiK01921
PhylomeDBiP0A6J8

Enzyme and pathway databases

UniPathwayi
UPA00219

BioCyciEcoCyc:DALADALALIGA-MONOMER
MetaCyc:DALADALALIGA-MONOMER

Miscellaneous databases

PROiPR:P0A6J8

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDDLA_ECOLI
AccessioniPrimary (citable) accession number: P0A6J8
Secondary accession number(s): P23844, Q2MC44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: November 7, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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