UniProtKB - P0A6J3 (CYSZ_ECOLI)
Protein
Sulfate transporter CysZ
Gene
cysZ
Organism
Escherichia coli (strain K12)
Status
Functioni
High affinity, high specificity proton-dependent sulfate transporter, which mediates sulfate uptake. Provides the sulfur source for the cysteine synthesis pathway. Does not transport thiosulfate.1 Publication
Activity regulationi
Inhibited by sulfite.1 Publication
Kineticsi
- KM=0.72 µM for sulfate1 Publication
- KM=1.3 µM for sulfate (in the presence of 5 µM sulfite)1 Publication
GO - Molecular functioni
- high-affinity sulfate:proton symporter activity Source: EcoCyc
- sulfate transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- cysteine biosynthetic process Source: EcoCyc
- sulfate assimilation Source: EcoCyc
- sulfate transmembrane transport Source: EcoCyc
Keywordsi
Biological process | Amino-acid biosynthesis, Cysteine biosynthesis, Sulfate transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10003-MONOMER |
Protein family/group databases
TCDBi | 2.A.121.1.1, the sulfate transporter (cysz) family |
Names & Taxonomyi
Protein namesi | Recommended name: Sulfate transporter CysZ1 PublicationUniRule annotation |
Gene namesi | Name:cysZ1 PublicationUniRule annotation Ordered Locus Names:b2413, JW2406 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 30 | CytoplasmicCuratedAdd BLAST | 30 | |
Transmembranei | 31 – 51 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 52 – 74 | PeriplasmicCuratedAdd BLAST | 23 | |
Transmembranei | 75 – 95 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 96 – 150 | CytoplasmicCuratedAdd BLAST | 55 | |
Transmembranei | 151 – 171 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 172 – 221 | PeriplasmicCuratedAdd BLAST | 50 | |
Transmembranei | 222 – 242 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 243 – 253 | Cytoplasmic1 PublicationAdd BLAST | 11 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204336 | 1 – 253 | Sulfate transporter CysZAdd BLAST | 253 |
Proteomic databases
jPOSTi | P0A6J3 |
PaxDbi | P0A6J3 |
PRIDEi | P0A6J3 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259702, 13 interactors |
STRINGi | 511145.b2413 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2981, Bacteria |
HOGENOMi | CLU_070331_1_0_6 |
InParanoidi | P0A6J3 |
PhylomeDBi | P0A6J3 |
Family and domain databases
HAMAPi | MF_00468, CysZ, 1 hit |
InterProi | View protein in InterPro IPR022985, Sulfate_CysZ |
i Sequence
Sequence statusi: Complete.
P0A6J3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSSFTSAPR SGFYYFAQGW KLVSQPGIRR FVILPLLVNI LLMGGAFWWL
60 70 80 90 100
FTQLDVWIPT LMSYVPDWLQ WLSYLLWPLA VISVLLVFGY FFSTIANWIA
110 120 130 140 150
APFNGLLAEQ LEARLTGATP PDTGIFGIMK DVPRIMKREW QKFAWYLPRA
160 170 180 190 200
IVLLILYFIP GIGQTVAPVL WFLFSAWMLA IQYCDYPFDN HKVPFKEMRT
210 220 230 240 250
ALRTRKITNM QFGALTSLFT MIPLLNLFIM PVAVCGATAM WVDCYRDKHA
MWR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75466.1 AP009048 Genomic DNA Translation: BAA16287.2 U74650 Genomic DNA Translation: AAB42060.1 M21451 Genomic DNA Translation: AAA23653.1 X12615 Genomic DNA Translation: CAA31136.1 |
PIRi | D65015, BVECCZ |
RefSeqi | NP_416908.1, NC_000913.3 WP_000254839.1, NZ_SSZK01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAC75466; AAC75466; b2413 BAA16287; BAA16287; BAA16287 |
GeneIDi | 946875 |
KEGGi | ecj:JW2406 eco:b2413 |
PATRICi | fig|1411691.4.peg.4318 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75466.1 AP009048 Genomic DNA Translation: BAA16287.2 U74650 Genomic DNA Translation: AAB42060.1 M21451 Genomic DNA Translation: AAA23653.1 X12615 Genomic DNA Translation: CAA31136.1 |
PIRi | D65015, BVECCZ |
RefSeqi | NP_416908.1, NC_000913.3 WP_000254839.1, NZ_SSZK01000005.1 |
3D structure databases
SMRi | P0A6J3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259702, 13 interactors |
STRINGi | 511145.b2413 |
Protein family/group databases
TCDBi | 2.A.121.1.1, the sulfate transporter (cysz) family |
Proteomic databases
jPOSTi | P0A6J3 |
PaxDbi | P0A6J3 |
PRIDEi | P0A6J3 |
Genome annotation databases
EnsemblBacteriai | AAC75466; AAC75466; b2413 BAA16287; BAA16287; BAA16287 |
GeneIDi | 946875 |
KEGGi | ecj:JW2406 eco:b2413 |
PATRICi | fig|1411691.4.peg.4318 |
Organism-specific databases
EchoBASEi | EB0003 |
Phylogenomic databases
eggNOGi | COG2981, Bacteria |
HOGENOMi | CLU_070331_1_0_6 |
InParanoidi | P0A6J3 |
PhylomeDBi | P0A6J3 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10003-MONOMER |
Miscellaneous databases
PROi | PR:P0A6J3 |
Family and domain databases
HAMAPi | MF_00468, CysZ, 1 hit |
InterProi | View protein in InterPro IPR022985, Sulfate_CysZ |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYSZ_ECOLI | |
Accessioni | P0A6J3Primary (citable) accession number: P0A6J3 Secondary accession number(s): P12610 P76962 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | March 29, 2005 | |
Last modified: | February 10, 2021 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families