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Protein

Sulfate transporter CysZ

Gene

cysZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

High affinity, high specificity proton-dependent sulfate transporter, which mediates sulfate uptake. Provides the sulfur source for the cysteine synthesis pathway. Does not transport thiosulfate.1 Publication

Enzyme regulationi

Inhibited by sulfite.1 Publication

Kineticsi

  1. KM=0.72 µM for sulfate1 Publication
  2. KM=1.3 µM for sulfate (in the presence of 5 µM sulfite)1 Publication

    GO - Molecular functioni

    • high-affinity sulfate:proton symporter activity Source: EcoCyc
    • sulfate transmembrane transporter activity Source: EcoCyc

    GO - Biological processi

    • cysteine biosynthetic process Source: EcoCyc
    • sulfate assimilation Source: EcoCyc
    • sulfate transmembrane transport Source: EcoCyc

    Keywordsi

    Biological processAmino-acid biosynthesis, Cysteine biosynthesis, Sulfate transport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10003-MONOMER

    Protein family/group databases

    TCDBi2.A.121.1.1 the sulfate transporter (cysz) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfate transporter CysZ1 PublicationUniRule annotation
    Gene namesi
    Name:cysZ1 PublicationUniRule annotation
    Ordered Locus Names:b2413, JW2406
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10003 cysZ

    Subcellular locationi

    • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 30CytoplasmicCuratedAdd BLAST30
    Transmembranei31 – 51HelicalUniRule annotationAdd BLAST21
    Topological domaini52 – 74PeriplasmicCuratedAdd BLAST23
    Transmembranei75 – 95HelicalUniRule annotationAdd BLAST21
    Topological domaini96 – 150CytoplasmicCuratedAdd BLAST55
    Transmembranei151 – 171HelicalUniRule annotationAdd BLAST21
    Topological domaini172 – 221PeriplasmicCuratedAdd BLAST50
    Transmembranei222 – 242HelicalUniRule annotationAdd BLAST21
    Topological domaini243 – 253Cytoplasmic1 PublicationAdd BLAST11

    GO - Cellular componenti

    • integral component of plasma membrane Source: EcoCyc
    • plasma membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002043361 – 253Sulfate transporter CysZAdd BLAST253

    Proteomic databases

    PaxDbiP0A6J3
    PRIDEiP0A6J3

    Interactioni

    Protein-protein interaction databases

    BioGridi4259702, 13 interactors
    STRINGi316385.ECDH10B_2578

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6J3
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CysZ family.UniRule annotationCurated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105EXN Bacteria
    COG2981 LUCA
    HOGENOMiHOG000273989
    InParanoidiP0A6J3
    KOiK06203
    OMAiQYIDYPA
    PhylomeDBiP0A6J3

    Family and domain databases

    HAMAPiMF_00468 CysZ, 1 hit
    InterProiView protein in InterPro
    IPR022985 Sulfate_CysZ

    Sequencei

    Sequence statusi: Complete.

    P0A6J3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVSSFTSAPR SGFYYFAQGW KLVSQPGIRR FVILPLLVNI LLMGGAFWWL
    60 70 80 90 100
    FTQLDVWIPT LMSYVPDWLQ WLSYLLWPLA VISVLLVFGY FFSTIANWIA
    110 120 130 140 150
    APFNGLLAEQ LEARLTGATP PDTGIFGIMK DVPRIMKREW QKFAWYLPRA
    160 170 180 190 200
    IVLLILYFIP GIGQTVAPVL WFLFSAWMLA IQYCDYPFDN HKVPFKEMRT
    210 220 230 240 250
    ALRTRKITNM QFGALTSLFT MIPLLNLFIM PVAVCGATAM WVDCYRDKHA

    MWR
    Length:253
    Mass (Da):29,305
    Last modified:March 29, 2005 - v1
    Checksum:i057C4908D9FBB453
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC75466.1
    AP009048 Genomic DNA Translation: BAA16287.2
    U74650 Genomic DNA Translation: AAB42060.1
    M21451 Genomic DNA Translation: AAA23653.1
    X12615 Genomic DNA Translation: CAA31136.1
    PIRiD65015 BVECCZ
    RefSeqiNP_416908.1, NC_000913.3
    WP_000254839.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC75466; AAC75466; b2413
    BAA16287; BAA16287; BAA16287
    GeneIDi946875
    KEGGiecj:JW2406
    eco:b2413
    PATRICifig|1411691.4.peg.4318

    Similar proteinsi

    Entry informationi

    Entry nameiCYSZ_ECOLI
    AccessioniPrimary (citable) accession number: P0A6J3
    Secondary accession number(s): P12610
    , P76533, P76961, P76962
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: April 25, 2018
    This is version 94 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

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