UniProtKB - P0A6J1 (CYSC_ECOLI)
Protein
Adenylyl-sulfate kinase
Gene
cysC
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the synthesis of activated sulfate.
Catalytic activityi
- EC:2.7.1.25
: hydrogen sulfide biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes sulfite from sulfate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD), Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
- Adenylyl-sulfate kinase (cysC), Adenylyl-sulfate kinase (cysC)
- Phosphoadenosine phosphosulfate reductase (cysH), Phosphoadenosine phosphosulfate reductase (cysH)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 109 | Phosphoserine intermediate1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 35 – 42 | ATPBy similarity | 8 |
GO - Molecular functioni
- adenylylsulfate kinase activity Source: EcoCyc
- ATP binding Source: UniProtKB-UniRule
- identical protein binding Source: EcoCyc
GO - Biological processi
- hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
- phosphorylation Source: EcoCyc
- sulfate assimilation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:ADENYLYLSULFKIN-MONOMER MetaCyc:ADENYLYLSULFKIN-MONOMER |
UniPathwayi | UPA00140;UER00205 |
Names & Taxonomyi
Protein namesi | Recommended name: Adenylyl-sulfate kinase (EC:2.7.1.25)Alternative name(s): APS kinase ATP adenosine-5'-phosphosulfate 3'-phosphotransferase Adenosine-5'-phosphosulfate kinase |
Gene namesi | Name:cysC Ordered Locus Names:b2750, JW2720 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000105909 | 2 – 201 | Adenylyl-sulfate kinaseAdd BLAST | 200 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P0A6J1 |
PRIDEi | P0A6J1 |
Interactioni
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4263423, 19 interactors |
IntActi | P0A6J1, 11 interactors |
STRINGi | 511145.b2750 |
Family & Domainsi
Sequence similaritiesi
Belongs to the APS kinase family.Curated
Phylogenomic databases
eggNOGi | COG0529, Bacteria |
HOGENOMi | CLU_046932_1_0_6 |
InParanoidi | P0A6J1 |
PhylomeDBi | P0A6J1 |
Family and domain databases
CDDi | cd02027, APSK, 1 hit |
HAMAPi | MF_00065, Adenylyl_sulf_kinase, 1 hit |
InterProi | View protein in InterPro IPR002891, APS_kinase IPR027417, P-loop_NTPase |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00455, apsK, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A6J1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALHDENVVW HSHPVTVQQR ELHHGHRGVV LWFTGLSGSG KSTVAGALEE
60 70 80 90 100
ALHKLGVSTY LLDGDNVRHG LCSDLGFSDA DRKENIRRVG EVANLMVEAG
110 120 130 140 150
LVVLTAFISP HRAERQMVRE RVGEGRFIEV FVDTPLAICE ARDPKGLYKK
160 170 180 190 200
ARAGELRNFT GIDSVYEAPE SAEIHLNGEQ LVTNLVQQLL DLLRQNDIIR
S
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 134 | T → Q in AAA23647 (PubMed:1316900).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74586 Genomic DNA Translation: AAA23647.1 M86936 Genomic DNA Translation: AAA23503.1 U29579 Genomic DNA Translation: AAA69260.1 U00096 Genomic DNA Translation: AAC75792.1 AP009048 Genomic DNA Translation: BAE76827.1 |
PIRi | B65056 |
RefSeqi | NP_417230.1, NC_000913.3 WP_001173673.1, NZ_STEB01000027.1 |
Genome annotation databases
EnsemblBacteriai | AAC75792; AAC75792; b2750 BAE76827; BAE76827; BAE76827 |
GeneIDi | 58389988 947221 |
KEGGi | ecj:JW2720 eco:b2750 |
PATRICi | fig|1411691.4.peg.3990 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74586 Genomic DNA Translation: AAA23647.1 M86936 Genomic DNA Translation: AAA23503.1 U29579 Genomic DNA Translation: AAA69260.1 U00096 Genomic DNA Translation: AAC75792.1 AP009048 Genomic DNA Translation: BAE76827.1 |
PIRi | B65056 |
RefSeqi | NP_417230.1, NC_000913.3 WP_001173673.1, NZ_STEB01000027.1 |
3D structure databases
SMRi | P0A6J1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263423, 19 interactors |
IntActi | P0A6J1, 11 interactors |
STRINGi | 511145.b2750 |
Proteomic databases
PaxDbi | P0A6J1 |
PRIDEi | P0A6J1 |
Genome annotation databases
EnsemblBacteriai | AAC75792; AAC75792; b2750 BAE76827; BAE76827; BAE76827 |
GeneIDi | 58389988 947221 |
KEGGi | ecj:JW2720 eco:b2750 |
PATRICi | fig|1411691.4.peg.3990 |
Organism-specific databases
EchoBASEi | EB0182 |
Phylogenomic databases
eggNOGi | COG0529, Bacteria |
HOGENOMi | CLU_046932_1_0_6 |
InParanoidi | P0A6J1 |
PhylomeDBi | P0A6J1 |
Enzyme and pathway databases
UniPathwayi | UPA00140;UER00205 |
BioCyci | EcoCyc:ADENYLYLSULFKIN-MONOMER MetaCyc:ADENYLYLSULFKIN-MONOMER |
Miscellaneous databases
PROi | PR:P0A6J1 |
Family and domain databases
CDDi | cd02027, APSK, 1 hit |
HAMAPi | MF_00065, Adenylyl_sulf_kinase, 1 hit |
InterProi | View protein in InterPro IPR002891, APS_kinase IPR027417, P-loop_NTPase |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00455, apsK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYSC_ECOLI | |
Accessioni | P0A6J1Primary (citable) accession number: P0A6J1 Secondary accession number(s): P23846 Q59389 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 115 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families