UniProtKB - P0A6F9 (CH10_ECOLI)
Co-chaperonin GroES
groES
Functioni
Together with the chaperonin GroEL, plays an essential role in assisting protein folding (PubMed:2897629, PubMed:2573517, PubMed:10532860, PubMed:1676490, PubMed:16751100, PubMed:18418386, PubMed:18987317, PubMed:20603018, PubMed:24816391).
The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding, probably by preventing aggregation and by entropically destabilizing folding intermediates (PubMed:16751100, PubMed:18418386, PubMed:18987317, PubMed:20603018, PubMed:24816391).
GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel (PubMed:9285585).
Regulates the ATPase activity of GroEL (PubMed:1676490, PubMed:1361169).
11 Publications(Microbial infection) Essential for the assembly of several bacteriophages.
2 PublicationsActivity regulationi
GO - Molecular functioni
- ATP binding Source: InterPro
- ATP hydrolysis activity Source: InterPro
- chaperone binding Source: GO_Central
- identical protein binding Source: EcoCyc
- metal ion binding Source: GO_Central
- unfolded protein binding Source: GO_Central
GO - Biological processi
- chaperone cofactor-dependent protein refolding Source: EcoCyc
- protein folding Source: CACAO
- response to heat Source: EcoliWiki
- virion assembly Source: EcoliWiki
Keywordsi
Molecular function | Chaperone |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10600-MONOMER |
SABIO-RKi | P0A6F9 |
Names & Taxonomyi
Protein namesi | Recommended name: Co-chaperonin GroESUniRule annotationCuratedAlternative name(s): 10 kDa chaperoninUniRule annotationCurated Chaperonin-101 PublicationUniRule annotation Short name: Cpn101 PublicationUniRule annotation |
Gene namesi | Name:groES1 PublicationUniRule annotation Synonyms:groS, mopB Ordered Locus Names:b4142, JW4102 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Note: Exclusively localized in foci, usually near 1 cell pole in mid-to-late exponential phase; polar localization depends on the minCDE operon. Foci form near midcell.
Cytosol
- cytosol Source: EcoCyc
- GroEL-GroES complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000174746 | 1 – 97 | Co-chaperonin GroESAdd BLAST | 97 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 34 | N6-succinyllysine1 Publication | 1 |
Proteomic databases
jPOSTi | P0A6F9 |
PaxDbi | P0A6F9 |
PRIDEi | P0A6F9 |
2D gel databases
SWISS-2DPAGEi | P0A6F9 |
Interactioni
Subunit structurei
Heptamer of 7 subunits arranged in a ring (PubMed:1361169, PubMed:9285585).
Interacts with the chaperonin GroEL (PubMed:1361169, PubMed:7638600, PubMed:7638601, PubMed:8618836, PubMed:8663256, PubMed:9285585, PubMed:25174333). Can form asymmetrical complexes, composed of one GroEL and one GroES, and symmetrical complexes, formed between one GroEL and two GroES oligomers (PubMed:1361169, PubMed:7638600, PubMed:7638601, PubMed:8618836, PubMed:8663256, PubMed:25174333). The asymmetrical complex is the functional unit (PubMed:7638600, PubMed:7638601, PubMed:25912285). It was suggested that the symmetric heterooligomer may represent a transient intermediate in the chaperonin protein folding cycle (PubMed:8618836, PubMed:8663256). Another study shows that the symmetric heterooligomers are substantially populated only in the presence of proteins that cannot be folded by the chaperonin (PubMed:25912285).
8 PublicationsBinary interactionsi
P0A6F9
With | #Exp. | IntAct |
---|---|---|
groL [P0A6F5] | 31 | EBI-369169,EBI-543750 |
itself | 2 | EBI-369169,EBI-369169 |
GO - Molecular functioni
- chaperone binding Source: GO_Central
- identical protein binding Source: EcoCyc
- unfolded protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 4262198, 222 interactors |
ComplexPortali | CPX-2113, GroEL-GroES complex |
DIPi | DIP-9835N |
IntActi | P0A6F9, 41 interactors |
MINTi | P0A6F9 |
STRINGi | 511145.b4142 |
Structurei
Secondary structure
3D structure databases
SMRi | P0A6F9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A6F9 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0234, Bacteria |
HOGENOMi | CLU_132825_1_1_6 |
InParanoidi | P0A6F9 |
PhylomeDBi | P0A6F9 |
Family and domain databases
CDDi | cd00320, cpn10, 1 hit |
Gene3Di | 2.30.33.40, 1 hit |
HAMAPi | MF_00580, CH10, 1 hit |
InterProi | View protein in InterPro IPR020818, Chaperonin_GroES IPR037124, Chaperonin_GroES_sf IPR018369, Chaprnonin_Cpn10_CS IPR011032, GroES-like_sf |
PANTHERi | PTHR10772, PTHR10772, 1 hit |
Pfami | View protein in Pfam PF00166, Cpn10, 1 hit |
PRINTSi | PR00297, CHAPERONIN10 |
SMARTi | View protein in SMART SM00883, Cpn10, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit |
PROSITEi | View protein in PROSITE PS00681, CHAPERONINS_CPN10, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE
60 70 80 90
NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 89 | S → N in CAA30738 (PubMed:2901493).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07850 Genomic DNA Translation: CAA30697.1 X07899 Genomic DNA Translation: CAA30738.1 U14003 Genomic DNA Translation: AAA97041.1 U00096 Genomic DNA Translation: AAC77102.1 AP009048 Genomic DNA Translation: BAE78144.1 |
PIRi | S03931, BVECGS |
RefSeqi | NP_418566.1, NC_000913.3 WP_001026276.1, NZ_STEB01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77102; AAC77102; b4142 BAE78144; BAE78144; BAE78144 |
GeneIDi | 67414760 948655 |
KEGGi | ecj:JW4102 eco:b4142 |
PATRICi | fig|1411691.4.peg.2558 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07850 Genomic DNA Translation: CAA30697.1 X07899 Genomic DNA Translation: CAA30738.1 U14003 Genomic DNA Translation: AAA97041.1 U00096 Genomic DNA Translation: AAC77102.1 AP009048 Genomic DNA Translation: BAE78144.1 |
PIRi | S03931, BVECGS |
RefSeqi | NP_418566.1, NC_000913.3 WP_001026276.1, NZ_STEB01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AON | X-ray | 3.00 | O/P/Q/R/S/T/U | 1-97 | [»] | |
1EGS | NMR | - | A | 19-27 | [»] | |
1GRU | electron microscopy | 12.50 | O/P/Q/R/S/T/U | 1-97 | [»] | |
1PCQ | X-ray | 2.81 | O/P/Q/R/S/T/U | 1-97 | [»] | |
1PF9 | X-ray | 2.99 | O/P/Q/R/S/T/U | 1-97 | [»] | |
1SVT | X-ray | 2.81 | O/P/Q/R/S/T/U | 1-97 | [»] | |
1SX4 | X-ray | 3.00 | O/P/Q/R/S/T/U | 1-97 | [»] | |
2C7C | electron microscopy | 7.70 | O/P/Q/R/S/T/U | 1-97 | [»] | |
2C7D | electron microscopy | 8.70 | O/P/Q/R/S/T/U | 1-97 | [»] | |
3WVL | X-ray | 3.79 | O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b | 1-97 | [»] | |
3ZPZ | electron microscopy | 8.90 | O/P/Q/R/S/T/U | 1-97 | [»] | |
3ZQ0 | electron microscopy | 9.20 | O/P/Q/R/S/T/U | 1-97 | [»] | |
3ZQ1 | electron microscopy | 15.90 | O/P/Q/R/S/T/U | 1-97 | [»] | |
5OPX | X-ray | 3.64 | 1/2/O/P/Q/R/S/T/U/V/W/X/Y/Z | 1-97 | [»] | |
SMRi | P0A6F9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262198, 222 interactors |
ComplexPortali | CPX-2113, GroEL-GroES complex |
DIPi | DIP-9835N |
IntActi | P0A6F9, 41 interactors |
MINTi | P0A6F9 |
STRINGi | 511145.b4142 |
2D gel databases
SWISS-2DPAGEi | P0A6F9 |
Proteomic databases
jPOSTi | P0A6F9 |
PaxDbi | P0A6F9 |
PRIDEi | P0A6F9 |
Genome annotation databases
EnsemblBacteriai | AAC77102; AAC77102; b4142 BAE78144; BAE78144; BAE78144 |
GeneIDi | 67414760 948655 |
KEGGi | ecj:JW4102 eco:b4142 |
PATRICi | fig|1411691.4.peg.2558 |
Organism-specific databases
EchoBASEi | EB0595 |
Phylogenomic databases
eggNOGi | COG0234, Bacteria |
HOGENOMi | CLU_132825_1_1_6 |
InParanoidi | P0A6F9 |
PhylomeDBi | P0A6F9 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10600-MONOMER |
SABIO-RKi | P0A6F9 |
Miscellaneous databases
EvolutionaryTracei | P0A6F9 |
PROi | PR:P0A6F9 |
Family and domain databases
CDDi | cd00320, cpn10, 1 hit |
Gene3Di | 2.30.33.40, 1 hit |
HAMAPi | MF_00580, CH10, 1 hit |
InterProi | View protein in InterPro IPR020818, Chaperonin_GroES IPR037124, Chaperonin_GroES_sf IPR018369, Chaprnonin_Cpn10_CS IPR011032, GroES-like_sf |
PANTHERi | PTHR10772, PTHR10772, 1 hit |
Pfami | View protein in Pfam PF00166, Cpn10, 1 hit |
PRINTSi | PR00297, CHAPERONIN10 |
SMARTi | View protein in SMART SM00883, Cpn10, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit |
PROSITEi | View protein in PROSITE PS00681, CHAPERONINS_CPN10, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CH10_ECOLI | |
Accessioni | P0A6F9Primary (citable) accession number: P0A6F9 Secondary accession number(s): P05380, Q2M6G2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | March 29, 2005 | |
Last modified: | February 23, 2022 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families