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Protein

Carbamoyl-phosphate synthase small chain

Gene

carA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase catalytic subunit (pyrB), Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC), Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei269NucleophileBy similarity1
Active sitei353By similarity1
Active sitei355By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00068;UER00171

UPA00070;UER00115

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene namesi
Name:carA
Synonyms:pyrA
Ordered Locus Names:Z0037, ECs0035
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001122751 – 382Carbamoyl-phosphate synthase small chainAdd BLAST382

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

STRINGi155864.Z0037

Structurei

3D structure databases

ProteinModelPortaliP0A6F2
SMRiP0A6F2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini193 – 380Glutamine amidotransferase type-1Add BLAST188

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 189CPSaseAdd BLAST189

Sequence similaritiesi

Belongs to the CarA family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C1M Bacteria
COG0505 LUCA
HOGENOMiHOG000038087
KOiK01956

Family and domain databases

CDDicd01744 GATase1_CPSase, 1 hit
Gene3Di3.40.50.880, 1 hit
3.50.30.20, 1 hit
HAMAPiMF_01209 CPSase_S_chain, 1 hit
InterProiView protein in InterPro
IPR006274 CarbamoylP_synth_ssu
IPR002474 CarbamoylP_synth_ssu_N
IPR036480 CarbP_synth_ssu_N_sf
IPR029062 Class_I_gatase-like
IPR035686 CPSase_GATase1
IPR017926 GATASE
PfamiView protein in Pfam
PF00988 CPSase_sm_chain, 1 hit
PF00117 GATase, 1 hit
SMARTiView protein in SMART
SM01097 CPSase_sm_chain, 1 hit
SUPFAMiSSF52021 SSF52021, 1 hit
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR01368 CPSaseIIsmall, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P0A6F2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR
60 70 80 90 100
QIVTLTYPHI GNVGTNDADE ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS
110 120 130 140 150
YLKRHNIVAI ADIDTRKLTR LLREKGAQNG CIIAGDNPDA ALALEKARAF
160 170 180 190 200
PGLNGMDLAK EVTTAEAYSW TQGSWTLTGG LPEAKKEDEL PFHVVAYDFG
210 220 230 240 250
AKRNILRMLV DRGCRLTIVP AQTSAEDVLK MNPDGIFLSN GPGDPAPCDY
260 270 280 290 300
AITAIQKFLE TDIPVFGICL GHQLLALASG AKTVKMKFGH HGGNHPVKDV
310 320 330 340 350
EKNVVMITAQ NHGFAVDEAT LPANLRVTHK SLFDGTLQGI HRTDKPAFSF
360 370 380
QGHPEASPGP HDAAPLFDHF IELIEQYRKT AK
Length:382
Mass (Da):41,431
Last modified:July 21, 1986 - v1
Checksum:i60BC26366417443F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG54334.1
BA000007 Genomic DNA Translation: BAB33458.1
PIRiC90633
RefSeqiNP_308062.1, NC_002695.1
WP_000597260.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG54334; AAG54334; Z0037
BAB33458; BAB33458; BAB33458
GeneIDi913431
KEGGiece:Z0037
ecs:ECs0035
PATRICifig|386585.9.peg.131

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG54334.1
BA000007 Genomic DNA Translation: BAB33458.1
PIRiC90633
RefSeqiNP_308062.1, NC_002695.1
WP_000597260.1, NZ_NOKN01000002.1

3D structure databases

ProteinModelPortaliP0A6F2
SMRiP0A6F2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z0037

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG54334; AAG54334; Z0037
BAB33458; BAB33458; BAB33458
GeneIDi913431
KEGGiece:Z0037
ecs:ECs0035
PATRICifig|386585.9.peg.131

Phylogenomic databases

eggNOGiENOG4105C1M Bacteria
COG0505 LUCA
HOGENOMiHOG000038087
KOiK01956

Enzyme and pathway databases

UniPathwayi
UPA00068;UER00171

UPA00070;UER00115

Family and domain databases

CDDicd01744 GATase1_CPSase, 1 hit
Gene3Di3.40.50.880, 1 hit
3.50.30.20, 1 hit
HAMAPiMF_01209 CPSase_S_chain, 1 hit
InterProiView protein in InterPro
IPR006274 CarbamoylP_synth_ssu
IPR002474 CarbamoylP_synth_ssu_N
IPR036480 CarbP_synth_ssu_N_sf
IPR029062 Class_I_gatase-like
IPR035686 CPSase_GATase1
IPR017926 GATASE
PfamiView protein in Pfam
PF00988 CPSase_sm_chain, 1 hit
PF00117 GATase, 1 hit
SMARTiView protein in SMART
SM01097 CPSase_sm_chain, 1 hit
SUPFAMiSSF52021 SSF52021, 1 hit
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR01368 CPSaseIIsmall, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCARA_ECO57
AccessioniPrimary (citable) accession number: P0A6F2
Secondary accession number(s): P00907
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 7, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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