UniProtKB - P0A6C5 (ARGA_ECOLI)
Protein
Amino-acid acetyltransferase
Gene
argA
Organism
Escherichia coli (strain K12)
Status
Functioni
Miscellaneous
The mutant strains shown below are all feedback-resistant (fbr) strains exhibiting enhanced arginine biosynthesis.1 Publication
Catalytic activityi
- EC:2.3.1.11 Publication
Activity regulationi
Feedback inhibition by L-arginine.1 Publication
: L-arginine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.1 PublicationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- Amino-acid acetyltransferase (argA), Amino-acid acetyltransferase (argA)
- Acetylglutamate kinase (argB), Acetylglutamate kinase (argB)
- N-acetyl-gamma-glutamyl-phosphate reductase (argC), N-acetyl-gamma-glutamyl-phosphate reductase (argC)
- Acetylornithine/succinyldiaminopimelate aminotransferase (argD), Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- acetyl-CoA:L-glutamate N-acetyltransferase activity Source: UniProtKB-UniRule
- glutamate N-acetyltransferase activity Source: EcoCyc
- methione N-acyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- arginine biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Amino-acid biosynthesis, Arginine biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:N-ACETYLTRANSFER-MONOMER MetaCyc:N-ACETYLTRANSFER-MONOMER |
BRENDAi | 2.3.1.1, 2026 |
SABIO-RKi | P0A6C5 |
UniPathwayi | UPA00068;UER00106 |
Names & Taxonomyi
Protein namesi | Recommended name: Amino-acid acetyltransferase (EC:2.3.1.1)Alternative name(s): N-acetylglutamate synthase Short name: AGS Short name: NAGS |
Gene namesi | Name:argA Ordered Locus Names:b2818, JW2786 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 15 | H → Y in EE17. 1 Publication | 1 | |
Mutagenesisi | 19 | Y → C in EE51. 1 Publication | 1 | |
Mutagenesisi | 54 | S → N in PT2M217. 1 Publication | 1 | |
Mutagenesisi | 58 | R → H in EE11. 1 Publication | 1 | |
Mutagenesisi | 287 | G → S in PT2M216. 1 Publication | 1 | |
Mutagenesisi | 432 | Q → R in PT2M217. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186790 | 1 – 443 | Amino-acid acetyltransferaseAdd BLAST | 443 |
Proteomic databases
PaxDbi | P0A6C5 |
PRIDEi | P0A6C5 |
Interactioni
Subunit structurei
Homohexamer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262306, 14 interactors |
DIPi | DIP-48037N |
IntActi | P0A6C5, 3 interactors |
STRINGi | 511145.b2818 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 296 – 443 | N-acetyltransferaseAdd BLAST | 148 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0548, Bacteria COG1246, Bacteria |
HOGENOMi | CLU_024773_0_0_6 |
InParanoidi | P0A6C5 |
PhylomeDBi | P0A6C5 |
Family and domain databases
CDDi | cd04237, AAK_NAGS-ABP, 1 hit |
Gene3Di | 3.40.1160.10, 1 hit |
HAMAPi | MF_01105, N_acetyl_glu_synth, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR016181, Acyl_CoA_acyltransferase IPR001048, Asp/Glu/Uridylate_kinase IPR000182, GNAT_dom IPR033719, NAGS_kin IPR010167, NH2A_AcTrfase |
PANTHERi | PTHR30602, PTHR30602, 2 hits |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF00583, Acetyltransf_1, 1 hit |
PIRSFi | PIRSF000423, ArgA, 1 hit |
SUPFAMi | SSF53633, SSF53633, 1 hit SSF55729, SSF55729, 1 hit |
TIGRFAMsi | TIGR01890, N-Ac-Glu-synth, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A6C5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVKERKTELV EGFRHSVPYI NTHRGKTFVI MLGGEAIEHE NFSSIVNDIG
60 70 80 90 100
LLHSLGIRLV VVYGARPQID ANLAAHHHEP LYHKNIRVTD AKTLELVKQA
110 120 130 140 150
AGTLQLDITA RLSMSLNNTP LQGAHINVVS GNFIIAQPLG VDDGVDYCHS
160 170 180 190 200
GRIRRIDEDA IHRQLDSGAI VLMGPVAVSV TGESFNLTSE EIATQLAIKL
210 220 230 240 250
KAEKMIGFCS SQGVTNDDGD IVSELFPNEA QARVEAQEEK GDYNSGTVRF
260 270 280 290 300
LRGAVKACRS GVRRCHLISY QEDGALLQEL FSRDGIGTQI VMESAEQIRR
310 320 330 340 350
ATINDIGGIL ELIRPLEQQG ILVRRSREQL EMEIDKFTII QRDNTTIACA
360 370 380 390 400
ALYPFPEEKI GEMACVAVHP DYRSSSRGEV LLERIAAQAK QSGLSKLFVL
410 420 430 440
TTRSIHWFQE RGFTPVDIDL LPESKKQLYN YQRKSKVLMA DLG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00492 Genomic DNA Translation: CAA68547.1 AF008115 Genomic DNA Translation: AAC23443.1 AF008116 Genomic DNA Translation: AAC23444.1 AF008117 Genomic DNA Translation: AAC23445.1 AF008118 Genomic DNA Translation: AAC23446.1 AF008119 Genomic DNA Translation: AAC23447.1 U29581 Genomic DNA Translation: AAB40465.1 U00096 Genomic DNA Translation: AAC75857.1 AP009048 Genomic DNA Translation: BAE76887.1 |
PIRi | A30372, XYECAA |
RefSeqi | NP_417295.1, NC_000913.3 WP_000237947.1, NZ_SSUV01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC75857; AAC75857; b2818 BAE76887; BAE76887; BAE76887 |
GeneIDi | 57730380 947289 |
KEGGi | ecj:JW2786 eco:b2818 |
PATRICi | fig|1411691.4.peg.3918 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00492 Genomic DNA Translation: CAA68547.1 AF008115 Genomic DNA Translation: AAC23443.1 AF008116 Genomic DNA Translation: AAC23444.1 AF008117 Genomic DNA Translation: AAC23445.1 AF008118 Genomic DNA Translation: AAC23446.1 AF008119 Genomic DNA Translation: AAC23447.1 U29581 Genomic DNA Translation: AAB40465.1 U00096 Genomic DNA Translation: AAC75857.1 AP009048 Genomic DNA Translation: BAE76887.1 |
PIRi | A30372, XYECAA |
RefSeqi | NP_417295.1, NC_000913.3 WP_000237947.1, NZ_SSUV01000026.1 |
3D structure databases
SMRi | P0A6C5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262306, 14 interactors |
DIPi | DIP-48037N |
IntActi | P0A6C5, 3 interactors |
STRINGi | 511145.b2818 |
Proteomic databases
PaxDbi | P0A6C5 |
PRIDEi | P0A6C5 |
Genome annotation databases
EnsemblBacteriai | AAC75857; AAC75857; b2818 BAE76887; BAE76887; BAE76887 |
GeneIDi | 57730380 947289 |
KEGGi | ecj:JW2786 eco:b2818 |
PATRICi | fig|1411691.4.peg.3918 |
Organism-specific databases
EchoBASEi | EB0061 |
Phylogenomic databases
eggNOGi | COG0548, Bacteria COG1246, Bacteria |
HOGENOMi | CLU_024773_0_0_6 |
InParanoidi | P0A6C5 |
PhylomeDBi | P0A6C5 |
Enzyme and pathway databases
UniPathwayi | UPA00068;UER00106 |
BioCyci | EcoCyc:N-ACETYLTRANSFER-MONOMER MetaCyc:N-ACETYLTRANSFER-MONOMER |
BRENDAi | 2.3.1.1, 2026 |
SABIO-RKi | P0A6C5 |
Miscellaneous databases
PROi | PR:P0A6C5 |
Family and domain databases
CDDi | cd04237, AAK_NAGS-ABP, 1 hit |
Gene3Di | 3.40.1160.10, 1 hit |
HAMAPi | MF_01105, N_acetyl_glu_synth, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR016181, Acyl_CoA_acyltransferase IPR001048, Asp/Glu/Uridylate_kinase IPR000182, GNAT_dom IPR033719, NAGS_kin IPR010167, NH2A_AcTrfase |
PANTHERi | PTHR30602, PTHR30602, 2 hits |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF00583, Acetyltransf_1, 1 hit |
PIRSFi | PIRSF000423, ArgA, 1 hit |
SUPFAMi | SSF53633, SSF53633, 1 hit SSF55729, SSF55729, 1 hit |
TIGRFAMsi | TIGR01890, N-Ac-Glu-synth, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARGA_ECOLI | |
Accessioni | P0A6C5Primary (citable) accession number: P0A6C5 Secondary accession number(s): O68009 Q2MA19 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families