UniProtKB - P0A6B7 (ISCS_ECOLI)
Cysteine desulfurase IscS
iscS
Functioni
Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins. Preferentially binds to disordered IscU on which the Fe-S is assembled, IscU converts to the structured state and then dissociates from IscS to transfer the Fe-S to an acceptor protein. Also functions as a selenium delivery protein in the pathway for the biosynthesis of selenophosphate. Transfers sulfur onto 'Cys-456' of ThiI and onto 'Cys-19' of TusA in transpersulfidation reactions.
10 PublicationsCatalytic activityi
- EC:2.8.1.7UniRule annotation1 Publication
Cofactori
Activity regulationi
Kineticsi
- KM=2.7 µM for L-cysteine1 Publication
: iron-sulfur cluster biosynthesis Pathwayi
This protein is involved in the pathway iron-sulfur cluster biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway iron-sulfur cluster biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 155 | Pyridoxal phosphateUniRule annotation | 1 | |
Binding sitei | 183 | Pyridoxal phosphateUniRule annotation1 Publication | 1 | |
Binding sitei | 243 | Pyridoxal phosphateUniRule annotation1 Publication | 1 | |
Active sitei | 328 | Cysteine persulfide intermediateUniRule annotation2 Publications | 1 | |
Metal bindingi | 328 | Iron-sulfur (2Fe-2S); via persulfide group; shared with IscUUniRule annotation | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-UniRule
- cysteine desulfurase activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
- pyridoxal phosphate binding Source: EcoCyc
- selenocysteine lyase activity Source: EcoCyc
- sulfur carrier activity Source: EcoCyc
GO - Biological processi
- [2Fe-2S] cluster assembly Source: UniProtKB-UniRule
- detection of UV Source: ComplexPortal
- iron-sulfur cluster assembly Source: EcoCyc
- L-cysteine catabolic process Source: ComplexPortal
- oxazole or thiazole biosynthetic process Source: EcoCyc
- selenocysteine catabolic process Source: ComplexPortal
- sulfur compound transport Source: ComplexPortal
- thiamine biosynthetic process Source: ComplexPortal
- tRNA 4-thiouridine biosynthesis Source: EcoCyc
- tRNA wobble position uridine thiolation Source: ComplexPortal
Keywordsi
Molecular function | Transferase |
Biological process | tRNA processing |
Ligand | 2Fe-2S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:G7325-MONOMER |
SABIO-RKi | P0A6B7 |
UniPathwayi | UPA00266 |
Names & Taxonomyi
Protein namesi | Recommended name: Cysteine desulfurase IscS1 PublicationUniRule annotation (EC:2.8.1.7UniRule annotation1 Publication)Alternative name(s): NifS protein homolog1 Publication ThiI transpersulfidase1 Publication TusA transpersulfidase1 Publication |
Gene namesi | Name:iscSUniRule annotation Synonyms:nuvC, yfhO, yzzO Ordered Locus Names:b2530, JW2514 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotationCurated
Cytosol
- cytosol Source: EcoCyc
- IscS-IscU complex Source: ComplexPortal
- IscS-TusA complex Source: ComplexPortal
- L-cysteine desulfurase complex Source: ComplexPortal
- sulfurtransferase complex Source: ComplexPortal
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 328 | C → A: Loss of cysteine desulfurization. 1 Publication | 1 | |
Mutagenesisi | 376 – 404 | Missing : Normal cysteine desulfurase activity, decreased binding to IscU, decreased sulfur transfer to IscU, decreased Fe-S cluster assembly. 1 PublicationAdd BLAST | 29 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000150264 | 1 – 404 | Cysteine desulfurase IscSAdd BLAST | 404 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 206 | N6-(pyridoxal phosphate)lysineUniRule annotation1 Publication | 1 |
Proteomic databases
jPOSTi | P0A6B7 |
PaxDbi | P0A6B7 |
PRIDEi | P0A6B7 |
Interactioni
Subunit structurei
Homodimer. The homodimer interacts with IscU and TusA, other S acceptors. Each subunit of the IscS dimer contacts a IscU monomer.
4 PublicationsBinary interactionsi
P0A6B7
Protein-protein interaction databases
BioGRIDi | 4263511, 579 interactors 851343, 3 interactors |
ComplexPortali | CPX-2136, L-cysteine desulfurase complex CPX-2139, iscS-tusA cysteine desulfurase complex CPX-2140, iscS-thiI sulfurtransferase complex CPX-2141, iscS-iscU iron-sulfur cluster assembly complex |
DIPi | DIP-29109N |
IntActi | P0A6B7, 47 interactors |
MINTi | P0A6B7 |
STRINGi | 511145.b2530 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P0A6B7 |
SASBDBi | P0A6B7 |
SMRi | P0A6B7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A6B7 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 75 – 76 | Pyridoxal phosphate bindingUniRule annotation | 2 | |
Regioni | 203 – 205 | Pyridoxal phosphate bindingUniRule annotation1 Publication | 3 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1104, Bacteria |
HOGENOMi | CLU_003433_0_2_6 |
InParanoidi | P0A6B7 |
OMAi | KGLYWAR |
PhylomeDBi | P0A6B7 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00331, Cys_desulf_IscS, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR020578, Aminotrans_V_PyrdxlP_BS IPR010240, Cys_deSase_IscS IPR016454, Cysteine_dSase IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR11601:SF34, PTHR11601:SF34, 1 hit |
Pfami | View protein in Pfam PF00266, Aminotran_5, 1 hit |
PIRSFi | PIRSF005572, NifS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR02006, IscS, 1 hit |
PROSITEi | View protein in PROSITE PS00595, AA_TRANSFER_CLASS_5, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKLPIYLDYS ATTPVDPRVA EKMMQFMTMD GTFGNPASRS HRFGWQAEEA
60 70 80 90 100
VDIARNQIAD LVGADPREIV FTSGATESDN LAIKGAANFY QKKGKHIITS
110 120 130 140 150
KTEHKAVLDT CRQLEREGFE VTYLAPQRNG IIDLKELEAA MRDDTILVSI
160 170 180 190 200
MHVNNEIGVV QDIAAIGEMC RARGIIYHVD ATQSVGKLPI DLSQLKVDLM
210 220 230 240 250
SFSGHKIYGP KGIGALYVRR KPRVRIEAQM HGGGHERGMR SGTLPVHQIV
260 270 280 290 300
GMGEAYRIAK EEMATEMERL RGLRNRLWNG IKDIEEVYLN GDLEHGAPNI
310 320 330 340 350
LNVSFNYVEG ESLIMALKDL AVSSGSACTS ASLEPSYVLR ALGLNDELAH
360 370 380 390 400
SSIRFSLGRF TTEEEIDYTI ELVRKSIGRL RDLSPLWEMY KQGVDLNSIE
WAHH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 | G → D AA sequence (PubMed:8663056).Curated | 1 | |
Sequence conflicti | 34 | G → F AA sequence (PubMed:8663056).Curated | 1 | |
Sequence conflicti | 37 – 38 | AS → NA AA sequence (PubMed:8663056).Curated | 2 | |
Sequence conflicti | 118 | G → L AA sequence (PubMed:8663056).Curated | 1 | |
Sequence conflicti | 126 | P → M AA sequence (PubMed:8663056).Curated | 1 | |
Sequence conflicti | 320 | L → I AA sequence (PubMed:8663056).Curated | 1 | |
Sequence conflicti | 326 – 327 | SA → YL AA sequence (PubMed:8663056).Curated | 2 | |
Sequence conflicti | 339 | L → V AA sequence (PubMed:8663056).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAT48142.1 AP009048 Genomic DNA Translation: BAA16424.1 |
RefSeqi | WP_001295373.1, NZ_STEB01000011.1 YP_026169.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48142; AAT48142; b2530 BAA16424; BAA16424; BAA16424 |
GeneIDi | 67416914 947004 |
KEGGi | ecj:JW2514 eco:b2530 |
PATRICi | fig|1411691.4.peg.4204 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAT48142.1 AP009048 Genomic DNA Translation: BAA16424.1 |
RefSeqi | WP_001295373.1, NZ_STEB01000011.1 YP_026169.1, NC_000913.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1P3W | X-ray | 2.10 | A/B | 1-404 | [»] | |
AlphaFoldDBi | P0A6B7 | |||||
SASBDBi | P0A6B7 | |||||
SMRi | P0A6B7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263511, 579 interactors 851343, 3 interactors |
ComplexPortali | CPX-2136, L-cysteine desulfurase complex CPX-2139, iscS-tusA cysteine desulfurase complex CPX-2140, iscS-thiI sulfurtransferase complex CPX-2141, iscS-iscU iron-sulfur cluster assembly complex |
DIPi | DIP-29109N |
IntActi | P0A6B7, 47 interactors |
MINTi | P0A6B7 |
STRINGi | 511145.b2530 |
Proteomic databases
jPOSTi | P0A6B7 |
PaxDbi | P0A6B7 |
PRIDEi | P0A6B7 |
Genome annotation databases
EnsemblBacteriai | AAT48142; AAT48142; b2530 BAA16424; BAA16424; BAA16424 |
GeneIDi | 67416914 947004 |
KEGGi | ecj:JW2514 eco:b2530 |
PATRICi | fig|1411691.4.peg.4204 |
Organism-specific databases
EchoBASEi | EB2542 |
Phylogenomic databases
eggNOGi | COG1104, Bacteria |
HOGENOMi | CLU_003433_0_2_6 |
InParanoidi | P0A6B7 |
OMAi | KGLYWAR |
PhylomeDBi | P0A6B7 |
Enzyme and pathway databases
UniPathwayi | UPA00266 |
BioCyci | EcoCyc:G7325-MONOMER |
SABIO-RKi | P0A6B7 |
Miscellaneous databases
EvolutionaryTracei | P0A6B7 |
PROi | PR:P0A6B7 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00331, Cys_desulf_IscS, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR020578, Aminotrans_V_PyrdxlP_BS IPR010240, Cys_deSase_IscS IPR016454, Cysteine_dSase IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR11601:SF34, PTHR11601:SF34, 1 hit |
Pfami | View protein in Pfam PF00266, Aminotran_5, 1 hit |
PIRSFi | PIRSF005572, NifS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR02006, IscS, 1 hit |
PROSITEi | View protein in PROSITE PS00595, AA_TRANSFER_CLASS_5, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ISCS_ECOLI | |
Accessioni | P0A6B7Primary (citable) accession number: P0A6B7 Secondary accession number(s): P39171 Q8XA86 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | May 25, 2022 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families