UniProtKB - P0A6A8 (ACP_ECOLI)
Protein
Acyl carrier protein
Gene
acpP
Organism
Escherichia coli (strain K12)
Status
Functioni
Carrier of the growing fatty acid chain in fatty acid biosynthesis.
: fatty acid biosynthesis Pathwayi
This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.
GO - Molecular functioni
- acyl binding Source: EcoliWiki
- acyl carrier activity Source: EcoliWiki
- lipid binding Source: CAFA
- phosphopantetheine binding Source: EcoliWiki
GO - Biological processi
- fatty acid biosynthetic process Source: EcoliWiki
- lipid A biosynthetic process Source: EcoliWiki
- lipid biosynthetic process Source: EcoliWiki
- response to drug Source: EcoliWiki
Keywordsi
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BioCyci | EcoCyc:EG50003-MONOMER MetaCyc:EG50003-MONOMER |
UniPathwayi | UPA00094 |
Names & Taxonomyi
Protein namesi | Recommended name: Acyl carrier proteinUniRule annotationShort name: ACPUniRule annotation Alternative name(s): Cytosolic-activating factor Short name: CAF Fatty acid synthase acyl carrier protein |
Gene namesi | Name:acpPUniRule annotation Ordered Locus Names:b1094, JW1080 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 37 | S → A or T: Loss of phosphopantetheinylation, and inhibition of cell growth. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3309006 |
DrugBanki | DB04450, Heptyl 1-Thiohexopyranoside DB08405, S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) HEPTANETHIOATE DB08404, S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) HEXANETHIOATE |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed3 Publications | |||
ChainiPRO_0000180134 | 2 – 78 | Acyl carrier proteinAdd BLAST | 77 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 37 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1 Publication | 1 |
Post-translational modificationi
4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.
Keywords - PTMi
Phosphopantetheine, PhosphoproteinProteomic databases
jPOSTi | P0A6A8 |
PaxDbi | P0A6A8 |
PRIDEi | P0A6A8 |
Interactioni
Subunit structurei
Interacts with MukB.
1 PublicationBinary interactionsi
Hide detailsP0A6A8
Protein-protein interaction databases
BioGRIDi | 4263360, 495 interactors |
DIPi | DIP-29374N |
IntActi | P0A6A8, 45 interactors |
STRINGi | 511145.b1094 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SASBDBi | P0A6A8 |
SMRi | P0A6A8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A6A8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 77 | CarrierPROSITE-ProRule annotationAdd BLAST | 76 |
Sequence similaritiesi
Belongs to the acyl carrier protein (ACP) family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0236, Bacteria |
HOGENOMi | CLU_108696_5_1_6 |
InParanoidi | P0A6A8 |
PhylomeDBi | P0A6A8 |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit |
HAMAPi | MF_01217, Acyl_carrier, 1 hit |
InterProi | View protein in InterPro IPR036736, ACP-like_sf IPR003231, Acyl_carrier IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site |
Pfami | View protein in Pfam PF00550, PP-binding, 1 hit |
SUPFAMi | SSF47336, SSF47336, 1 hit |
TIGRFAMsi | TIGR00517, acyl_carrier, 1 hit |
PROSITEi | View protein in PROSITE PS50075, CARRIER, 1 hit PS00012, PHOSPHOPANTETHEINE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A6A8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTIEERVKK IIGEQLGVKQ EEVTNNASFV EDLGADSLDT VELVMALEEE
60 70
FDTEIPDEEA EKITTVQAAI DYINGHQA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 25 | N → D AA sequence (PubMed:4882207).Curated | 1 | |
Sequence conflicti | 44 | V → I AA sequence (PubMed:3549687).Curated | 1 | |
Sequence conflicti | 71 | D → V in AAB27925 (PubMed:8359454).Curated | 1 | |
Sequence conflicti | 76 | H → N in AAB27925 (PubMed:8359454).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84991 Genomic DNA Translation: AAA23740.1 S65033 Genomic DNA Translation: AAB27925.2 U00096 Genomic DNA Translation: AAC74178.1 AP009048 Genomic DNA Translation: BAA35902.1 Z34979 Genomic DNA No translation available. |
PIRi | C42147, AYEC |
RefSeqi | NP_415612.1, NC_000913.3 WP_000103754.1, NZ_STEB01000016.1 |
Genome annotation databases
EnsemblBacteriai | AAC74178; AAC74178; b1094 BAA35902; BAA35902; BAA35902 |
GeneIDi | 53888910 58461214 60373896 944805 |
KEGGi | ecj:JW1080 eco:b1094 |
PATRICi | fig|1411691.4.peg.1174 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84991 Genomic DNA Translation: AAA23740.1 S65033 Genomic DNA Translation: AAB27925.2 U00096 Genomic DNA Translation: AAC74178.1 AP009048 Genomic DNA Translation: BAA35902.1 Z34979 Genomic DNA No translation available. |
PIRi | C42147, AYEC |
RefSeqi | NP_415612.1, NC_000913.3 WP_000103754.1, NZ_STEB01000016.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ACP | NMR | - | A | 2-78 | [»] | |
1L0H | X-ray | 2.00 | A | 1-77 | [»] | |
1L0I | X-ray | 1.20 | A | 1-78 | [»] | |
1T8K | X-ray | 1.10 | A | 2-78 | [»] | |
2FAC | X-ray | 1.76 | A/B | 2-78 | [»] | |
2FAD | X-ray | 1.60 | A/B | 2-78 | [»] | |
2FAE | X-ray | 1.55 | A/B | 2-78 | [»] | |
2FHS | X-ray | 2.70 | C | 1-78 | [»] | |
2K92 | NMR | - | A | 2-78 | [»] | |
2K93 | NMR | - | A | 2-78 | [»] | |
2K94 | NMR | - | A | 2-78 | [»] | |
3EJB | X-ray | 2.00 | A/C/E/G | 1-78 | [»] | |
3EJD | X-ray | 2.10 | A/C/E/G | 1-78 | [»] | |
3EJE | X-ray | 2.10 | A/C/E/G | 1-78 | [»] | |
3NY7 | X-ray | 1.92 | B | 1-78 | [»] | |
5KOF | X-ray | 2.40 | C/D | 1-78 | [»] | |
5USR | X-ray | 3.09 | I/J/K/L | 1-78 | [»] | |
5VCB | X-ray | 4.10 | D/E/F/J/K/L/P/Q/R/V/W/X/d/e/f | 2-78 | [»] | |
5WGB | X-ray | 2.75 | C | 2-78 | [»] | |
5WKP | X-ray | 3.15 | C/G | 2-78 | [»] | |
6DFL | X-ray | 2.40 | B | 3-74 | [»] | |
6N3P | X-ray | 2.50 | G/H/I/J/K/L | 2-78 | [»] | |
6OKC | X-ray | 1.55 | C/D | 1-78 | [»] | |
6OKF | X-ray | 2.50 | C/D | 1-78 | [»] | |
6OKG | X-ray | 2.30 | B | 1-78 | [»] | |
6OLT | X-ray | 2.35 | B | 1-78 | [»] | |
6U0J | X-ray | 1.90 | B | 1-78 | [»] | |
6UXE | X-ray | 1.57 | C | 2-78 | [»] | |
6W1D | X-ray | 1.79 | C | 2-78 | [»] | |
6W98 | electron microscopy | 2.90 | B | 1-78 | [»] | |
6WI2 | X-ray | 1.95 | C | 2-78 | [»] | |
6WIH | X-ray | 1.90 | C | 2-78 | [»] | |
SASBDBi | P0A6A8 | |||||
SMRi | P0A6A8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263360, 495 interactors |
DIPi | DIP-29374N |
IntActi | P0A6A8, 45 interactors |
STRINGi | 511145.b1094 |
Chemistry databases
ChEMBLi | CHEMBL3309006 |
DrugBanki | DB04450, Heptyl 1-Thiohexopyranoside DB08405, S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) HEPTANETHIOATE DB08404, S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) HEXANETHIOATE |
Proteomic databases
jPOSTi | P0A6A8 |
PaxDbi | P0A6A8 |
PRIDEi | P0A6A8 |
Genome annotation databases
EnsemblBacteriai | AAC74178; AAC74178; b1094 BAA35902; BAA35902; BAA35902 |
GeneIDi | 53888910 58461214 60373896 944805 |
KEGGi | ecj:JW1080 eco:b1094 |
PATRICi | fig|1411691.4.peg.1174 |
Organism-specific databases
EchoBASEi | EB4297 |
Phylogenomic databases
eggNOGi | COG0236, Bacteria |
HOGENOMi | CLU_108696_5_1_6 |
InParanoidi | P0A6A8 |
PhylomeDBi | P0A6A8 |
Enzyme and pathway databases
UniPathwayi | UPA00094 |
BioCyci | EcoCyc:EG50003-MONOMER MetaCyc:EG50003-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0A6A8 |
PROi | PR:P0A6A8 |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit |
HAMAPi | MF_01217, Acyl_carrier, 1 hit |
InterProi | View protein in InterPro IPR036736, ACP-like_sf IPR003231, Acyl_carrier IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site |
Pfami | View protein in Pfam PF00550, PP-binding, 1 hit |
SUPFAMi | SSF47336, SSF47336, 1 hit |
TIGRFAMsi | TIGR00517, acyl_carrier, 1 hit |
PROSITEi | View protein in PROSITE PS50075, CARRIER, 1 hit PS00012, PHOSPHOPANTETHEINE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACP_ECOLI | |
Accessioni | P0A6A8Primary (citable) accession number: P0A6A8 Secondary accession number(s): P02901, Q53352 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families