UniProtKB - P0A698 (UVRA_ECOLI)
Protein
UvrABC system protein A
Gene
uvrA
Organism
Escherichia coli (strain K12)
Status
Functioni
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.UniRule annotation
Miscellaneous
Binds about 2 zinc atoms/molecule.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 31 – 38 | ATP | 8 | |
Zinc fingeri | 253 – 280 | C4-typeUniRule annotationAdd BLAST | 28 | |
Nucleotide bindingi | 640 – 647 | ATP | 8 | |
Zinc fingeri | 740 – 766 | C4-typeUniRule annotationAdd BLAST | 27 |
GO - Molecular functioni
- ATPase activity Source: EcoCyc
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: EcoCyc
- excinuclease ABC activity Source: UniProtKB-UniRule
- identical protein binding Source: IntAct
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA repair Source: EcoCyc
- nucleotide-excision repair Source: UniProtKB-UniRule
- response to radiation Source: EcoCyc
- SOS response Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding, Excision nuclease |
Biological process | DNA damage, DNA excision, DNA repair, SOS response |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11061-MONOMER MetaCyc:EG11061-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: UvrABC system protein AUniRule annotationShort name: UvrA proteinUniRule annotation Alternative name(s): Excinuclease ABC subunit AUniRule annotation |
Gene namesi | Name:uvrAUniRule annotation Synonyms:dinE Ordered Locus Names:b4058, JW4019 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: EcoCyc
Other locations
- excinuclease repair complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 253 | C → A, H or S: Reduced activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093049 | 1 – 940 | UvrABC system protein AAdd BLAST | 940 |
Proteomic databases
jPOSTi | P0A698 |
PaxDbi | P0A698 |
PRIDEi | P0A698 |
Interactioni
Subunit structurei
Forms a heterotetramer with UvrB during the search for lesions (By similarity).
Interacts with TRCF (Mfd). UvrB and TRCF binding to UvrA could be mutually exclusive.
UniRule annotation1 PublicationBinary interactionsi
Hide detailsP0A698
With | #Exp. | IntAct |
---|---|---|
atl [P0AFP2] | 2 | EBI-552091,EBI-560039 |
mfd [P30958] | 2 | EBI-552091,EBI-554211 |
itself | 2 | EBI-552091,EBI-552091 |
uvrB [P0A8F8] | 7 | EBI-552091,EBI-552176 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262670, 52 interactors 852853, 2 interactors |
ComplexPortali | CPX-2151, UvrAB complex CPX-2155, TRCF-UvrA complex |
DIPi | DIP-35876N |
IntActi | P0A698, 17 interactors |
STRINGi | 511145.b4058 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A698 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 310 – 587 | ABC transporter 1UniRule annotationAdd BLAST | 278 | |
Domaini | 607 – 937 | ABC transporter 2UniRule annotationAdd BLAST | 331 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 253 – 280 | C4-typeUniRule annotationAdd BLAST | 28 | |
Zinc fingeri | 740 – 766 | C4-typeUniRule annotationAdd BLAST | 27 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | COG0178, Bacteria |
HOGENOMi | CLU_001370_0_2_6 |
InParanoidi | P0A698 |
PhylomeDBi | P0A698 |
Family and domain databases
HAMAPi | MF_00205, UvrA, 1 hit |
InterProi | View protein in InterPro IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase IPR004602, UvrA IPR041552, UvrA_DNA-bd IPR041102, UvrA_inter |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit PF17755, UvrA_DNA-bind, 1 hit PF17760, UvrA_inter, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR00630, uvra, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A698-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDKIEVRGAR THNLKNINLV IPRDKLIVVT GLSGSGKSSL AFDTLYAEGQ
60 70 80 90 100
RRYVESLSAY ARQFLSLMEK PDVDHIEGLS PAISIEQKST SHNPRSTVGT
110 120 130 140 150
ITEIHDYLRL LFARVGEPRC PDHDVPLAAQ TVSQMVDNVL SQPEGKRLML
160 170 180 190 200
LAPIIKERKG EHTKTLENLA SQGYIRARID GEVCDLSDPP KLELQKKHTI
210 220 230 240 250
EVVVDRFKVR DDLTQRLAES FETALELSGG TAVVADMDDP KAEELLFSAN
260 270 280 290 300
FACPICGYSM RELEPRLFSF NNPAGACPTC DGLGVQQYFD PDRVIQNPEL
310 320 330 340 350
SLAGGAIRGW DRRNFYYFQM LKSLADHYKF DVEAPWGSLS ANVHKVVLYG
360 370 380 390 400
SGKENIEFKY MNDRGDTSIR RHPFEGVLHN MERRYKETES SAVREELAKF
410 420 430 440 450
ISNRPCASCE GTRLRREARH VYVENTPLPA ISDMSIGHAM EFFNNLKLAG
460 470 480 490 500
QRAKIAEKIL KEIGDRLKFL VNVGLNYLTL SRSAETLSGG EAQRIRLASQ
510 520 530 540 550
IGAGLVGVMY VLDEPSIGLH QRDNERLLGT LIHLRDLGNT VIVVEHDEDA
560 570 580 590 600
IRAADHVIDI GPGAGVHGGE VVAEGPLEAI MAVPESLTGQ YMSGKRKIEV
610 620 630 640 650
PKKRVPANPE KVLKLTGARG NNLKDVTLTL PVGLFTCITG VSGSGKSTLI
660 670 680 690 700
NDTLFPIAQR QLNGATIAEP APYRDIQGLE HFDKVIDIDQ SPIGRTPRSN
710 720 730 740 750
PATYTGVFTP VRELFAGVPE SRARGYTPGR FSFNVRGGRC EACQGDGVIK
760 770 780 790 800
VEMHFLPDIY VPCDQCKGKR YNRETLEIKY KGKTIHEVLD MTIEEAREFF
810 820 830 840 850
DAVPALARKL QTLMDVGLTY IRLGQSATTL SGGEAQRVKL ARELSKRGTG
860 870 880 890 900
QTLYILDEPT TGLHFADIQQ LLDVLHKLRD QGNTIVVIEH NLDVIKTADW
910 920 930 940
IVDLGPEGGS GGGEILVSGT PETVAECEAS HTARFLKPML
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13495 Genomic DNA Translation: AAA24754.1 U00006 Genomic DNA Translation: AAC43152.1 U00096 Genomic DNA Translation: AAC77028.1 AP009048 Genomic DNA Translation: BAE78060.1 X01621 Genomic DNA Translation: CAA25764.1 J01721 Genomic DNA Translation: AAA24753.1 |
PIRi | A23869, BVECUA |
RefSeqi | NP_418482.1, NC_000913.3 WP_000357740.1, NZ_STEB01000022.1 |
Genome annotation databases
EnsemblBacteriai | AAC77028; AAC77028; b4058 BAE78060; BAE78060; BAE78060 |
GeneIDi | 58391765 948559 |
KEGGi | ecj:JW4019 eco:b4058 |
PATRICi | fig|1411691.4.peg.2647 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13495 Genomic DNA Translation: AAA24754.1 U00006 Genomic DNA Translation: AAC43152.1 U00096 Genomic DNA Translation: AAC77028.1 AP009048 Genomic DNA Translation: BAE78060.1 X01621 Genomic DNA Translation: CAA25764.1 J01721 Genomic DNA Translation: AAA24753.1 |
PIRi | A23869, BVECUA |
RefSeqi | NP_418482.1, NC_000913.3 WP_000357740.1, NZ_STEB01000022.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4DFC | X-ray | 2.80 | B/D | 131-250 | [»] | |
SMRi | P0A698 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262670, 52 interactors 852853, 2 interactors |
ComplexPortali | CPX-2151, UvrAB complex CPX-2155, TRCF-UvrA complex |
DIPi | DIP-35876N |
IntActi | P0A698, 17 interactors |
STRINGi | 511145.b4058 |
Proteomic databases
jPOSTi | P0A698 |
PaxDbi | P0A698 |
PRIDEi | P0A698 |
Genome annotation databases
EnsemblBacteriai | AAC77028; AAC77028; b4058 BAE78060; BAE78060; BAE78060 |
GeneIDi | 58391765 948559 |
KEGGi | ecj:JW4019 eco:b4058 |
PATRICi | fig|1411691.4.peg.2647 |
Organism-specific databases
EchoBASEi | EB1054 |
Phylogenomic databases
eggNOGi | COG0178, Bacteria |
HOGENOMi | CLU_001370_0_2_6 |
InParanoidi | P0A698 |
PhylomeDBi | P0A698 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11061-MONOMER MetaCyc:EG11061-MONOMER |
Miscellaneous databases
PROi | PR:P0A698 |
Family and domain databases
HAMAPi | MF_00205, UvrA, 1 hit |
InterProi | View protein in InterPro IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase IPR004602, UvrA IPR041552, UvrA_DNA-bd IPR041102, UvrA_inter |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit PF17755, UvrA_DNA-bind, 1 hit PF17760, UvrA_inter, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR00630, uvra, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UVRA_ECOLI | |
Accessioni | P0A698Primary (citable) accession number: P0A698 Secondary accession number(s): P07671, P76788, Q2M6P6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families