UniProtKB - P0A4V5 (A85C_MYCBO)
Diacylglycerol acyltransferase/mycolyltransferase Ag85C
fbpC
Functioni
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria to fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).
By similarityCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 170 | NucleophileBy similarity | 1 | |
Binding sitei | 170 | SubstrateBy similarity | 1 | |
Binding sitei | 198 | SubstrateBy similarity | 1 | |
Active sitei | 274 | By similarity | 1 | |
Active sitei | 306 | By similarity | 1 |
GO - Molecular functioni
- diacylglycerol O-acyltransferase activity Source: UniProtKB-EC
- trehalose O-mycolyltransferase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Acyltransferase, Transferase |
Protein family/group databases
ESTHERi | myctu-a85c, A85-Mycolyl-transferase |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:fbpC Synonyms:mpt45 Ordered Locus Names:BQ2027_MB0134C |
Organismi | Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) |
Taxonomic identifieri | 233413 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 45 | Sequence analysisAdd BLAST | 45 | |
ChainiPRO_0000000224 | 46 – 340 | Diacylglycerol acyltransferase/mycolyltransferase Ag85CAdd BLAST | 295 |
Interactioni
Subunit structurei
Homodimer.
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 86 – 87 | Substrate bindingBy similarity | 2 | |
Regioni | 102 – 112 | Fibronectin-bindingAdd BLAST | 11 | |
Regioni | 276 – 279 | Substrate bindingBy similarity | 4 | |
Regioni | 306 – 308 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
OMAi | RGNGQNY |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase-like |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MTFFEQVRRL RSAATTLPRR LAIAAMGAVL VYGLVGTFGG PATAGAFSRP
60 70 80 90 100
GLPVEYLQVP SASMGRDIKV QFQGGGPHAV YLLDGLRAQD DYNGWDINTP
110 120 130 140 150
AFEEYYQSGL SVIMPVGGQS SFYTDWYQPS QSNGQNYTYK WETFLTREMP
160 170 180 190 200
AWLQANKGVS PTGNAAVGLS MSGGSALILA AYYPQQFPYA ASLSGFLNPS
210 220 230 240 250
EGWWPTLIGL AMNDSGGYNA NSMWGPSSDP AWKRNDPMVQ IPRLVANNTR
260 270 280 290 300
IWVYCGNGTP SDLGGDNIPA KFLEGLTLRT NQTFRDTYAA DGGRNGVFNF
310 320 330 340
PPNGTHSWPY WNEQLVAMKA DIQHVLNGAT PPAAPAAPAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | LT708304 Genomic DNA Translation: SIT98556.1 |
RefSeqi | NP_853801.1, NC_002945.3 WP_003400908.1, NC_002945.4 |
Genome annotation databases
GeneIDi | 45424095 |
PATRICi | fig|233413.5.peg.151 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | LT708304 Genomic DNA Translation: SIT98556.1 |
RefSeqi | NP_853801.1, NC_002945.3 WP_003400908.1, NC_002945.4 |
3D structure databases
SMRi | P0A4V5 |
ModBasei | Search... |
Protein family/group databases
ESTHERi | myctu-a85c, A85-Mycolyl-transferase |
Genome annotation databases
GeneIDi | 45424095 |
PATRICi | fig|233413.5.peg.151 |
Phylogenomic databases
OMAi | RGNGQNY |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase-like |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | A85C_MYCBO | |
Accessioni | P0A4V5Primary (citable) accession number: P0A4V5 Secondary accession number(s): A0A1R3XUE5 X2BE42 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | March 15, 2005 | |
Last modified: | February 23, 2022 | |
This is version 84 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |