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Protein

Parathion hydrolase

Gene

opd

Organism
Brevundimonas diminuta (Pseudomonas diminuta)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates. All of the phosphate triesters found to be substrates are synthetic compounds. The identity of any naturally occurring substrate for the enzyme is unknown. Has no detectable activity with phosphate monoesters or diesters and no activity as an esterase or protease. It catalyzes the hydrolysis of the insecticide paraoxon at a rate approaching the diffusion limit and thus appears to be optimally evolved for utilizing this synthetic substrate.

Catalytic activityi

An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55Zinc 1; via tele nitrogenCombined sources1 Publication1
Metal bindingi57Zinc 1; via tele nitrogenCombined sources1 Publication1
Metal bindingi169Zinc 1; via carbamate groupCombined sources1 Publication1
Metal bindingi169Zinc 2; via carbamate groupCombined sources1 Publication1
Metal bindingi201Zinc 2; via pros nitrogenCombined sources1 Publication1
Metal bindingi230Zinc 2; via tele nitrogenCombined sources1 Publication1
Metal bindingi301Zinc 1Combined sources1 Publication1

GO - Molecular functioni

  • aryldialkylphosphatase activity Source: CACAO
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3322
BRENDAi3.1.1.2 982
3.1.8.1 982
SABIO-RKiP0A434

Names & Taxonomyi

Protein namesi
Recommended name:
Parathion hydrolase (EC:3.1.8.1)
Alternative name(s):
Phosphotriesterase
Short name:
PTE
Gene namesi
Name:opd
Encoded oniPlasmid pCMS10 Publication
OrganismiBrevundimonas diminuta (Pseudomonas diminuta)
Taxonomic identifieri293 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeBrevundimonas

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Biotechnological usei

Has attracted interest because of its potential use in the detoxification of chemical waste and warfare agents and its ability to degrade agricultural pesticides such as parathion.

Chemistry databases

DrugBankiDB02138 Diethyl 4-Methylbenzylphosphonate
DB02127 Methylphosphonic Acid Diisopropyl Ester
DB03347 Triethyl Phosphate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Tat-type signalPROSITE-ProRule annotation1 PublicationAdd BLAST29
ChainiPRO_000002986030 – 365Parathion hydrolaseAdd BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei169N6-carboxylysinePROSITE-ProRule annotationCombined sources1 Publication1

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1365
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0A434
SMRiP0A434
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A434

Family & Domainsi

Sequence similaritiesi

Belongs to the metallo-dependent hydrolases superfamily. Phosphotriesterase family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

CDDicd00530 PTE, 1 hit
InterProiView protein in InterPro
IPR017947 AryldialkylPase_Zn-BS
IPR032466 Metal_Hydrolase
IPR001559 Phosphotriesterase
IPR006311 TAT_signal
PANTHERiPTHR10819 PTHR10819, 1 hit
PfamiView protein in Pfam
PF02126 PTE, 1 hit
SUPFAMiSSF51556 SSF51556, 1 hit
PROSITEiView protein in PROSITE
PS01322 PHOSPHOTRIESTERASE_1, 1 hit
PS51347 PHOSPHOTRIESTERASE_2, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A434-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQTRRVVLKS AAAAGTLLGG LAGCASVAGS IGTGDRINTV RGPITISEAG
60 70 80 90 100
FTLTHEHICG SSAGFLRAWP EFFGSRKALA EKAVRGLRRA RAAGVRTIVD
110 120 130 140 150
VSTFDIGRDV SLLAEVSRAA DVHIVAATGL WFDPPLSMRL RSVEELTQFF
160 170 180 190 200
LREIQYGIED TGIRAGIIKV ATTGKATPFQ ELVLKAAARA SLATGVPVTT
210 220 230 240 250
HTAASQRDGE QQAAIFESEG LSPSRVCIGH SDDTDDLSYL TALAARGYLI
260 270 280 290 300
GLDHIPHSAI GLEDNASASA LLGIRSWQTR ALLIKALIDQ GYMKQILVSN
310 320 330 340 350
DWLFGFSSYV TNIMDVMDRV NPDGMAFIPL RVIPFLREKG VPQETLAGIT
360
VTNPARFLSP TLRAS
Length:365
Mass (Da):39,004
Last modified:March 15, 2005 - v1
Checksum:i41FF8E4B029B46DC
GO

Sequence cautioni

The sequence AAA98299 differs from that shown. Reason: Frameshift at several positions.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20392 Genomic DNA Translation: AAA98299.1 Frameshift.
PIRiA28214

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20392 Genomic DNA Translation: AAA98299.1 Frameshift.
PIRiA28214

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DPMX-ray2.10A/B36-363[»]
1EYWX-ray1.90A35-365[»]
1EZ2X-ray1.90A/B35-365[»]
1HZYX-ray1.30A/B34-365[»]
1I0BX-ray1.30A/B34-365[»]
1I0DX-ray1.30A/B34-365[»]
1JGMX-ray1.30A/B30-365[»]
1PSCX-ray2.00A/B1-365[»]
1PTAX-ray2.10A36-362[»]
1QW7X-ray1.90A/B30-365[»]
2O4MX-ray1.64A/B/C/P34-364[»]
2O4QX-ray1.95A/B/K/P34-364[»]
2OB3X-ray1.04A/B35-364[»]
2OQLX-ray1.80A/B35-365[»]
3CAKX-ray1.83A/B35-365[»]
3CS2X-ray1.95A/B/K/P34-364[»]
3E3HX-ray2.15A/B30-365[»]
3UPMX-ray1.95A/B35-361[»]
3UR2X-ray2.00A/B35-363[»]
3UR5X-ray1.60A/B35-361[»]
3URAX-ray1.88A/B35-361[»]
3URBX-ray1.77A/B35-361[»]
3URNX-ray1.95A/B35-361[»]
3URQX-ray2.10A/B35-361[»]
4E3TX-ray1.65A/B34-365[»]
4GY0X-ray1.85A/B34-365[»]
4GY1X-ray1.50A/B34-365[»]
4ZSTX-ray2.01A/B30-365[»]
4ZSUX-ray2.01A/B30-365[»]
ProteinModelPortaliP0A434
SMRiP0A434
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB02138 Diethyl 4-Methylbenzylphosphonate
DB02127 Methylphosphonic Acid Diisopropyl Ester
DB03347 Triethyl Phosphate

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3322
BRENDAi3.1.1.2 982
3.1.8.1 982
SABIO-RKiP0A434

Miscellaneous databases

EvolutionaryTraceiP0A434

Family and domain databases

CDDicd00530 PTE, 1 hit
InterProiView protein in InterPro
IPR017947 AryldialkylPase_Zn-BS
IPR032466 Metal_Hydrolase
IPR001559 Phosphotriesterase
IPR006311 TAT_signal
PANTHERiPTHR10819 PTHR10819, 1 hit
PfamiView protein in Pfam
PF02126 PTE, 1 hit
SUPFAMiSSF51556 SSF51556, 1 hit
PROSITEiView protein in PROSITE
PS01322 PHOSPHOTRIESTERASE_1, 1 hit
PS51347 PHOSPHOTRIESTERASE_2, 1 hit
PS51318 TAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOPD_BREDI
AccessioniPrimary (citable) accession number: P0A434
Secondary accession number(s): P13739, P16648
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: July 18, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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