UniProtKB - P0A3Y5 (KKA3_ENTFL)
Protein
Aminoglycoside 3'-phosphotransferase
Gene
aphA
Organism
Enterococcus faecalis (Streptococcus faecalis)
Status
Functioni
Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.
Catalytic activityi
- EC:2.7.1.95
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 190 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kanamycin kinase activity Source: UniProtKB-EC
GO - Biological processi
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Antibiotic resistance |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
SABIO-RKi | P0A3Y5 |
Names & Taxonomyi
Protein namesi | Recommended name: Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)Alternative name(s): APH(3')III Kanamycin kinase, type III Neomycin-kanamycin phosphotransferase type III |
Gene namesi | Name:aphA |
Encoded oni | Plasmid pJH1 |
Organismi | Enterococcus faecalis (Streptococcus faecalis) |
Taxonomic identifieri | 1351 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Enterococcaceae › Enterococcus |
Pathology & Biotechi
Chemistry databases
DrugBanki | DB04395, Phosphoaminophosphonic Acid-Adenylate Ester |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204805 | 1 – 264 | Aminoglycoside 3'-phosphotransferaseAdd BLAST | 264 |
Proteomic databases
PRIDEi | P0A3Y5 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0A3Y5 |
SMRi | P0A3Y5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A3Y5 |
Family & Domainsi
Sequence similaritiesi
Belongs to the aminoglycoside phosphotransferase family.Curated
Phylogenomic databases
OrthoDBi | 1457558at2 |
Family and domain databases
CDDi | cd05150, APH, 1 hit |
InterProi | View protein in InterPro IPR002575, Aminoglycoside_PTrfase IPR024165, Kan/Strep_kinase IPR011009, Kinase-like_dom_sf |
Pfami | View protein in Pfam PF01636, APH, 1 hit |
PIRSFi | PIRSF000706, Kanamycin_kin, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A3Y5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKMRISPEL KKLIEKYRCV KDTEGMSPAK VYKLVGENEN LYLKMTDSRY
60 70 80 90 100
KGTTYDVERE KDMMLWLEGK LPVPKVLHFE RHDGWSNLLM SEADGVLCSE
110 120 130 140 150
EYEDEQSPEK IIELYAECIR LFHSIDISDC PYTNSLDSRL AELDYLLNND
160 170 180 190 200
LADVDCENWE EDTPFKDPRE LYDFLKTEKP EEELVFSHGD LGDSNIFVKD
210 220 230 240 250
GKVSGFIDLG RSGRADKWYD IAFCVRSIRE DIGEEQYVEL FFDLLGIKPD
260
WEKIKYYILL DELF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01547 Genomic DNA Translation: CAA24789.1 X99398 Genomic DNA Translation: CAA67773.1 |
PIRi | A00665, PKSOJF |
RefSeqi | WP_001096887.1, NZ_WYAD01000043.1 YP_783930.1, NC_008445.1 |
Genome annotation databases
GeneIDi | 58729489 |
KEGGi | ag:CAA24789 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01547 Genomic DNA Translation: CAA24789.1 X99398 Genomic DNA Translation: CAA67773.1 |
PIRi | A00665, PKSOJF |
RefSeqi | WP_001096887.1, NZ_WYAD01000043.1 YP_783930.1, NC_008445.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1J7I | X-ray | 3.20 | A | 1-264 | [»] | |
1J7L | X-ray | 2.20 | A/B | 1-264 | [»] | |
1J7U | X-ray | 2.40 | A/B | 1-264 | [»] | |
1L8T | X-ray | 2.40 | A | 2-264 | [»] | |
2B0Q | X-ray | 2.70 | A | 2-264 | [»] | |
2BKK | X-ray | 2.15 | A/C | 1-264 | [»] | |
3Q2J | X-ray | 2.15 | A/B | 1-264 | [»] | |
3TM0 | X-ray | 2.10 | A | 2-264 | [»] | |
BMRBi | P0A3Y5 | |||||
SMRi | P0A3Y5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
DrugBanki | DB04395, Phosphoaminophosphonic Acid-Adenylate Ester |
Proteomic databases
PRIDEi | P0A3Y5 |
Genome annotation databases
GeneIDi | 58729489 |
KEGGi | ag:CAA24789 |
Phylogenomic databases
OrthoDBi | 1457558at2 |
Enzyme and pathway databases
SABIO-RKi | P0A3Y5 |
Miscellaneous databases
EvolutionaryTracei | P0A3Y5 |
PROi | PR:P0A3Y5 |
Family and domain databases
CDDi | cd05150, APH, 1 hit |
InterProi | View protein in InterPro IPR002575, Aminoglycoside_PTrfase IPR024165, Kan/Strep_kinase IPR011009, Kinase-like_dom_sf |
Pfami | View protein in Pfam PF01636, APH, 1 hit |
PIRSFi | PIRSF000706, Kanamycin_kin, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KKA3_ENTFL | |
Accessioni | P0A3Y5Primary (citable) accession number: P0A3Y5 Secondary accession number(s): P00554 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 10, 2021 | |
This is version 86 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, PlasmidDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families