UniProtKB - P0A3B2 (BIPA_ECO27)
Protein
50S ribosomal subunit assembly factor BipA
Gene
bipA
Organism
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Status
Functioni
A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA (By similarity). GTPase that impacts interactions between enteropathogenic E.coli (EPEC) and epithelial cells and also has an effect on motility (PubMed:9622352).UniRule annotation1 Publication
Miscellaneous
The initial events following infection of human cells by EPEC bacteria include the destruction of microvilli and the accumulation of actin underneath the adherent bacteria (part of the attaching and effacing lesion).1 Publication
Catalytic activityi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 15 – 20 | GTPUniRule annotation | 6 | |
Nucleotide bindingi | 128 – 131 | GTPUniRule annotation | 4 |
GO - Molecular functioni
- GTPase activity Source: InterPro
- GTP binding Source: UniProtKB-KW
- rRNA binding Source: UniProtKB-KW
- tRNA binding Source: UniProtKB-KW
GO - Biological processi
- pathogenesis Source: UniProtKB-KW
- ribosome biogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, RNA-binding, rRNA-binding, tRNA-binding |
Biological process | Ribosome biogenesis, Virulence |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: 50S ribosomal subunit assembly factor BipAUniRule annotation (EC:3.6.5.-UniRule annotation1 Publication)Alternative name(s): GTP-binding protein BipAUniRule annotation GTP-binding protein BipA/TypA Ribosome-dependent GTPase BipA Tyrosine phosphorylated protein A1 Publication |
Gene namesi | Name:bipA1 PublicationUniRule annotation Synonyms:o5911 Publication, typA1 Publication Ordered Locus Names:E2348C_4177 |
Organismi | Escherichia coli O127:H6 (strain E2348/69 / EPEC) |
Taxonomic identifieri | 574521 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation Note: Binds to ribosomes.UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Deletion alters the pattern of protein synthesis during both exponential growth and carbon starvation (PubMed:9642082). No longer alters host cytoskeleton; upon infection of HeLa cells microvilli are not disrupted (although some thicken), bacteria adhere to host cells but actin does not accumulate beneath them. 100-fold increased sensitivity of bacteria to human BPI. Increased bacterial cell motililty, increased secretion of flagellin gene (fliC) (PubMed:9622352). Decreased accumulation in infected HeLa cells of a phosphotyrosine protein that might be bacterial Tir, enhanced Ca2+ efflux from infected HeLa cells (PubMed:9765944).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000091551 | 1 – 607 | 50S ribosomal subunit assembly factor BipAAdd BLAST | 607 |
Post-translational modificationi
Phosphorylated on tyrosine, probably by autophosphorylation (PubMed:9622352, PubMed:9642082) (Probable). Phosphorylation is strongly activated by proteins present in strain E2348/69 / EPEC / MAR001 but not K12 / DH5 alpha. Phosphorylation increases GTPase activity (PubMed:9622352).1 Publication2 Publications
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Purifies as a 125-130 kDa band, also copurifies with RNA polymerase.
1 PublicationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 198 | tr-type GUniRule annotationAdd BLAST | 196 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. BipA subfamily.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_017016_4_0_6 |
OMAi | SMLFTIN |
Family and domain databases
CDDi | cd03710, BipA_TypA_C, 1 hit |
Gene3Di | 2.40.50.250, 1 hit |
HAMAPi | MF_00849, BipA, 1 hit |
InterProi | View protein in InterPro IPR035651, BipA_V IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR000795, TF_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR042116, TypA/BipA_C IPR006298, TypA_GTP-bd |
PANTHERi | PTHR42908:SF8, PTHR42908:SF8, 1 hit |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit TIGR01394, TypA_BipA, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A3B2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIEKLRNIAI IAHVDHGKTT LVDKLLQQSG TFDSRAETQE RVMDSNDLEK
60 70 80 90 100
ERGITILAKN TAIKWNDYRI NIVDTPGHAD FGGEVERVMS MVDSVLLVVD
110 120 130 140 150
AFDGPMPQTR FVTKKAFAYG LKPIVVINKV DRPGARPDWV VDQVFDLFVN
160 170 180 190 200
LDATDEQLDF PIVYASALNG IAGLDHEDMA EDMTPLYQAI VDHVPAPDVD
210 220 230 240 250
LDGPFQMQIS QLDYNSYVGV IGIGRIKRGK VKPNQQVTII DSEGKTRNAK
260 270 280 290 300
VGKVLGHLGL ERIETDLAEA GDIVAITGLG ELNISDTVCD TQNVEALPAL
310 320 330 340 350
SVDEPTVSMF FCVNTSPFCG KEGKFVTSRQ ILDRLNKELV HNVALRVEET
360 370 380 390 400
EDADAFRVSG RGELHLSVLI ENMRREGFEL AVSRPKVIFR EIDGRKQEPY
410 420 430 440 450
ENVTLDVEEQ HQGSVMQALG ERKGDLKNMN PDGKGRVRLD YVIPSRGLIG
460 470 480 490 500
FRSEFMTMTS GTGLLYSTFS HYDDVRPGEV GQRQNGVLIS NGQGKAVAFA
510 520 530 540 550
LFGLQDRGKL FLGHGAEVYE GQIIGIHSRS NDLTVNCLTG KKLTNMRASG
560 570 580 590 600
TDEAVVLVPP IRMTLEQALE FIDDDELVEV TPTSIRIRKR HLTENDRRRA
NRAPKDD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 151 | L → F in AAC13722 (PubMed:9642082).Curated | 1 | |
Sequence conflicti | 409 | E → Q in AAC13722 (PubMed:9642082).Curated | 1 | |
Sequence conflicti | 573 | D → H in AAC13722 (PubMed:9642082).Curated | 1 | |
Sequence conflicti | 576 | E → K in AAC13722 (PubMed:9642082).Curated | 1 | |
Sequence conflicti | 583 | T → I in AAC13722 (PubMed:9642082).Curated | 1 | |
Sequence conflicti | 589 – 607 | Missing in AAC13722 (PubMed:9642082).CuratedAdd BLAST | 19 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 489 | I → T in strain: MAR001. | 1 | |
Natural varianti | 497 | V → A in strain: MAR001. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ224871 Genomic DNA Translation: CAA12172.1 AJ278218 Genomic DNA Translation: CAC20136.1 FM180568 Genomic DNA Translation: CAS11725.1 AF058333 Genomic DNA Translation: AAC13722.1 |
RefSeqi | WP_000570668.1, NC_011601.1 |
Genome annotation databases
EnsemblBacteriai | CAS11725; CAS11725; E2348C_4177 |
GeneIDi | 49586844 |
KEGGi | ecg:E2348C_4177 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ224871 Genomic DNA Translation: CAA12172.1 AJ278218 Genomic DNA Translation: CAC20136.1 FM180568 Genomic DNA Translation: CAS11725.1 AF058333 Genomic DNA Translation: AAC13722.1 |
RefSeqi | WP_000570668.1, NC_011601.1 |
3D structure databases
SMRi | P0A3B2 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | CAS11725; CAS11725; E2348C_4177 |
GeneIDi | 49586844 |
KEGGi | ecg:E2348C_4177 |
Phylogenomic databases
HOGENOMi | CLU_017016_4_0_6 |
OMAi | SMLFTIN |
Family and domain databases
CDDi | cd03710, BipA_TypA_C, 1 hit |
Gene3Di | 2.40.50.250, 1 hit |
HAMAPi | MF_00849, BipA, 1 hit |
InterProi | View protein in InterPro IPR035651, BipA_V IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR000795, TF_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR042116, TypA/BipA_C IPR006298, TypA_GTP-bd |
PANTHERi | PTHR42908:SF8, PTHR42908:SF8, 1 hit |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit TIGR01394, TypA_BipA, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BIPA_ECO27 | |
Accessioni | P0A3B2Primary (citable) accession number: P0A3B2 Secondary accession number(s): B7UNJ3 Q9EXN7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | March 15, 2005 | |
Last modified: | December 2, 2020 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families