UniProtKB - P0A379 (CR3AA_BACTT)
Protein
Pesticidal crystal protein Cry3Aa
Gene
cry3Aa
Organism
Bacillus thuringiensis subsp. tenebrionis
Status
Functioni
Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera.
Miscellaneous
Toxic segment of the protein is located in the N-terminus.
GO - Molecular functioni
- signaling receptor binding Source: InterPro
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- pathogenesis Source: UniProtKB-KW
- sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Keywordsi
Molecular function | Toxin |
Biological process | Sporulation, Virulence |
Names & Taxonomyi
Protein namesi | Recommended name: Pesticidal crystal protein Cry3AaAlternative name(s): 73 kDa crystal protein Crystaline entomocidal protoxin Insecticidal delta-endotoxin CryIIIA(a) |
Gene namesi | Name:cry3Aa Synonyms:bt13, cry3A, cryC, cryIIIa, cryIIIA(a) |
Organismi | Bacillus thuringiensis subsp. tenebrionis |
Taxonomic identifieri | 1444 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › |
Pathology & Biotechi
Biotechnological usei
Introduced by genetic manipulation and expressed in insect-resistant potato by Monsanto.
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000006345 | 1 – 57 | Removed in mature formAdd BLAST | 57 | |
ChainiPRO_0000006346 | 58 – 644 | Pesticidal crystal protein Cry3AaAdd BLAST | 587 |
Proteomic databases
PRIDEi | P0A379 |
Expressioni
Developmental stagei
The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A379 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A379 |
Family & Domainsi
Sequence similaritiesi
Belongs to the delta endotoxin family.Curated
Family and domain databases
Gene3Di | 1.20.190.10, 1 hit 2.100.10.10, 1 hit 2.60.120.260, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR038979, Pest_crys IPR005638, Pest_crys_C IPR005639, Pest_crys_N IPR036716, Pest_crys_N_sf IPR001178, Pest_cryst_cen_dom IPR036399, Pest_cryst_cen_dom_sf |
PANTHERi | PTHR37003, PTHR37003, 1 hit |
Pfami | View protein in Pfam PF03944, Endotoxin_C, 1 hit PF00555, Endotoxin_M, 1 hit PF03945, Endotoxin_N, 1 hit |
SUPFAMi | SSF49785, SSF49785, 1 hit SSF51096, SSF51096, 1 hit SSF56849, SSF56849, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A379-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNPNNRSEHD TIKTTENNEV PTNHVQYPLA ETPNPTLEDL NYKEFLRMTA
60 70 80 90 100
DNNTEALDSS TTKDVIQKGI SVVGDLLGVV GFPFGGALVS FYTNFLNTIW
110 120 130 140 150
PSEDPWKAFM EQVEALMDQK IADYAKNKAL AELQGLQNNV EDYVSALSSW
160 170 180 190 200
QKNPVSSRNP HSQGRIRELF SQAESHFRNS MPSFAISGYE VLFLTTYAQA
210 220 230 240 250
ANTHLFLLKD AQIYGEEWGY EKEDIAEFYK RQLKLTQEYT DHCVKWYNVG
260 270 280 290 300
LDKLRGSSYE SWVNFNRYRR EMTLTVLDLI ALFPLYDVRL YPKEVKTELT
310 320 330 340 350
RDVLTDPIVG VNNLRGYGTT FSNIENYIRK PHLFDYLHRI QFHTRFQPGY
360 370 380 390 400
YGNDSFNYWS GNYVSTRPSI GSNDIITSPF YGNKSSEPVQ NLEFNGEKVY
410 420 430 440 450
RAVANTNLAV WPSAVYSGVT KVEFSQYNDQ TDEASTQTYD SKRNVGAVSW
460 470 480 490 500
DSIDQLPPET TDEPLEKGYS HQLNYVMCFL MQGSRGTIPV LTWTHKSVDF
510 520 530 540 550
FNMIDSKKIT QLPLVKAYKL QSGASVVAGP RFTGGDIIQC TENGSAATIY
560 570 580 590 600
VTPDVSYSQK YRARIHYAST SQITFTLSLD GAPFNQYYFD KTINKGDTLT
610 620 630 640
YNSFNLASFS TPFELSGNNL QIGVTGLSAG DKVYIDKIEF IPVN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 544 | G → A in AAA22542 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00420 Genomic DNA Translation: CAA68482.1 J02978 Genomic DNA Translation: AAA22541.1 M30503 Genomic DNA Translation: AAA22542.1 U10985 Genomic DNA Translation: AAC43266.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00420 Genomic DNA Translation: CAA68482.1 J02978 Genomic DNA Translation: AAA22541.1 M30503 Genomic DNA Translation: AAA22542.1 U10985 Genomic DNA Translation: AAC43266.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DLC | X-ray | 2.50 | A | 61-644 | [»] | |
4QX0 | X-ray | 2.80 | A | 58-644 | [»] | |
4QX1 | X-ray | 2.80 | A | 58-644 | [»] | |
4QX2 | X-ray | 2.90 | A | 58-644 | [»] | |
4QX3 | X-ray | 2.90 | A | 58-644 | [»] | |
SMRi | P0A379 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PRIDEi | P0A379 |
Miscellaneous databases
EvolutionaryTracei | P0A379 |
Family and domain databases
Gene3Di | 1.20.190.10, 1 hit 2.100.10.10, 1 hit 2.60.120.260, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR038979, Pest_crys IPR005638, Pest_crys_C IPR005639, Pest_crys_N IPR036716, Pest_crys_N_sf IPR001178, Pest_cryst_cen_dom IPR036399, Pest_cryst_cen_dom_sf |
PANTHERi | PTHR37003, PTHR37003, 1 hit |
Pfami | View protein in Pfam PF03944, Endotoxin_C, 1 hit PF00555, Endotoxin_M, 1 hit PF03945, Endotoxin_N, 1 hit |
SUPFAMi | SSF49785, SSF49785, 1 hit SSF51096, SSF51096, 1 hit SSF56849, SSF56849, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CR3AA_BACTT | |
Accessioni | P0A379Primary (citable) accession number: P0A379 Secondary accession number(s): P07130, P21255 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | April 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 61 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Genetically modified foodDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families