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UniProtKB - P0A334 (KCSA_STRLI)
Protein
pH-gated potassium channel KcsA
Gene
kcsA
Organism
Streptomyces lividans
Status
Functioni
Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K+ > Rb+ > NH4+ >> Na+ > Li+.
1 PublicationMiscellaneous
The amino acids 62-79 are situated in the membrane and are important for channel structure and properties.
GO - Molecular functioni
- identical protein binding Source: IntAct
- voltage-gated potassium channel activity Source: InterPro
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel |
Biological process | Ion transport, Transport |
Protein family/group databases
TCDBi | 1.A.1.1.1, the voltage-gated ion channel (vic) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: pH-gated potassium channel KcsAAlternative name(s): Streptomyces lividans K+ channel Short name: SKC1 |
Gene namesi | Name:kcsA Synonyms:skc1 |
Organismi | Streptomyces lividans |
Taxonomic identifieri | 1916 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces |
Subcellular locationi
Plasma membrane
Plasma Membrane
- voltage-gated potassium channel complex Source: InterPro
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 27 | CytoplasmicAdd BLAST | 27 | |
Transmembranei | 28 – 50 | HelicalAdd BLAST | 23 | |
Topological domaini | 51 – 61 | ExtracellularAdd BLAST | 11 | |
Intramembranei | 62 – 72 | Helical; Pore-formingAdd BLAST | 11 | |
Intramembranei | 73 – 80 | Pore-forming | 8 | |
Topological domaini | 81 – 87 | Extracellular | 7 | |
Transmembranei | 88 – 111 | HelicalAdd BLAST | 24 | |
Topological domaini | 112 – 160 | CytoplasmicAdd BLAST | 49 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells grow slower and to lower myceliar densities.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 71 | E → A: Prevents channel inactivation. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB07416, (2S)-2-(BUTYRYLOXY)-3-HYDROXYPROPYL NONANOATE DB01851, Tetrabutylammonium Ion DB08837, Tetraethylammonium |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054102 | 1 – 160 | pH-gated potassium channel KcsAAdd BLAST | 160 |
Interactioni
Subunit structurei
Homotetramer.
2 PublicationsBinary interactionsi
P0A334
With | #Exp. | IntAct |
---|---|---|
itself | 37 | EBI-7262059,EBI-7262059 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
DIPi | DIP-29626N |
MINTi | P0A334 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0A334 |
BMRBi | P0A334 |
PCDDBi | P0A334 |
SMRi | P0A334 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A334 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 75 – 80 | Selectivity filter | 6 |
Domaini
The cytoplasmic C-terminus is involved in the gating mechanism.
Sequence similaritiesi
Belongs to the potassium channel family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
InterProi | View protein in InterPro IPR013099, K_chnl_dom IPR028325, VG_K_chnl |
PANTHERi | PTHR11537, PTHR11537, 1 hit |
Pfami | View protein in Pfam PF07885, Ion_trans_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A334-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPPMLSGLLA RLVKLLLGRH GSALHWRAAG AATVLLVIVL LAGSYLAVLA
60 70 80 90 100
ERGAPGAQLI TYPRALWWSV ETATTVGYGD LYPVTLWGRL VAVVVMVAGI
110 120 130 140 150
TSFGLVTAAL ATWFVGREQE RRGHFVRHSE KAAEEAYTRT TRALHERFDR
160
LERMLDDNRR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z37969 Genomic DNA Translation: CAA86025.1 |
PIRi | S60172 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z37969 Genomic DNA Translation: CAA86025.1 |
PIRi | S60172 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BL8 | X-ray | 3.20 | A/B/C/D | 23-119 | [»] | |
1F6G | NMR | - | A/B/C/D | 1-160 | [»] | |
1J95 | X-ray | 2.80 | A/B/C/D | 1-125 | [»] | |
1JQ1 | NMR | - | A/B/C/D | 86-119 | [»] | |
1JQ2 | NMR | - | A/B/C/D | 86-119 | [»] | |
1JVM | X-ray | 2.80 | A/B/C/D | 1-125 | [»] | |
1K4C | X-ray | 2.00 | C | 1-124 | [»] | |
1K4D | X-ray | 2.30 | C | 1-124 | [»] | |
1R3I | X-ray | 2.40 | C | 1-124 | [»] | |
1R3J | X-ray | 1.90 | C | 1-124 | [»] | |
1R3K | X-ray | 2.80 | C | 1-124 | [»] | |
1R3L | X-ray | 2.41 | C | 1-124 | [»] | |
1ZWI | X-ray | 2.50 | C | 22-123 | [»] | |
2A9H | NMR | - | A/B/C/D | 1-132 | [»] | |
2ATK | X-ray | 2.50 | C | 1-124 | [»] | |
2BOB | X-ray | 2.76 | C | 1-124 | [»] | |
2BOC | X-ray | 3.01 | C | 1-124 | [»] | |
2DWD | X-ray | 2.60 | C | 22-124 | [»] | |
2DWE | X-ray | 2.50 | C | 22-124 | [»] | |
2H8P | X-ray | 2.25 | C | 22-78 | [»] | |
D | 80-122 | [»] | ||||
2HG5 | X-ray | 2.75 | C | 22-78 | [»] | |
D | 80-122 | [»] | ||||
2HJF | X-ray | 2.90 | C | 22-124 | [»] | |
2HVJ | X-ray | 2.75 | C | 1-124 | [»] | |
2HVK | X-ray | 1.90 | C | 1-124 | [»] | |
2IH1 | X-ray | 2.40 | C | 3-122 | [»] | |
2IH3 | X-ray | 1.72 | C | 3-122 | [»] | |
2ITC | X-ray | 3.20 | C | 1-124 | [»] | |
2ITD | X-ray | 2.70 | C | 1-124 | [»] | |
2JK5 | X-ray | 2.40 | C | 1-124 | [»] | |
2NLJ | X-ray | 2.52 | C | 1-124 | [»] | |
2P7T | X-ray | 2.05 | C | 22-124 | [»] | |
2QTO | X-ray | 3.20 | A/B/C/D | 23-119 | [»] | |
2W0F | X-ray | 2.40 | C | 1-124 | [»] | |
3EFF | X-ray | 3.80 | K/L/M/N | 22-160 | [»] | |
3F5W | X-ray | 3.30 | C | 21-124 | [»] | |
3F7V | X-ray | 3.20 | C | 21-124 | [»] | |
3F7Y | X-ray | 3.40 | C | 21-124 | [»] | |
3FB5 | X-ray | 2.80 | C | 21-124 | [»] | |
3FB6 | X-ray | 3.00 | C | 21-124 | [»] | |
3FB7 | X-ray | 3.30 | C | 21-124 | [»] | |
3FB8 | X-ray | 3.40 | C | 21-124 | [»] | |
3GB7 | X-ray | 2.85 | C | 1-124 | [»] | |
3HPL | X-ray | 3.20 | C | 1-124 | [»] | |
3IFX | Other | 3.56 | A/B/C/D | 1-123 | [»] | |
3IGA | X-ray | 2.75 | C | 1-124 | [»] | |
3OGC | X-ray | 3.80 | C | 2-124 | [»] | |
3OR6 | X-ray | 2.70 | C | 22-124 | [»] | |
3OR7 | X-ray | 2.30 | C | 22-124 | [»] | |
3PJS | X-ray | 3.80 | K/L/M/N | 22-160 | [»] | |
3STL | X-ray | 2.40 | C | 22-124 | [»] | |
3STZ | X-ray | 2.50 | C | 23-124 | [»] | |
4LBE | X-ray | 2.75 | C | 2-124 | [»] | |
4LCU | X-ray | 2.75 | C | 2-124 | [»] | |
4MSW | X-ray | 2.06 | C | 22-124 | [»] | |
4UUJ | X-ray | 2.40 | C | 22-124 | [»] | |
5E1A | X-ray | 3.40 | C | 4-124 | [»] | |
5EBL | X-ray | 2.30 | C | 1-125 | [»] | |
5EBM | X-ray | 2.50 | C | 1-125 | [»] | |
5EBW | X-ray | 2.30 | C | 1-123 | [»] | |
5EC1 | X-ray | 2.75 | C | 1-125 | [»] | |
5EC2 | X-ray | 2.30 | C | 1-125 | [»] | |
5J9P | X-ray | 2.85 | C | 22-117 | [»] | |
5VK6 | X-ray | 2.25 | C | 26-121 | [»] | |
5VKE | X-ray | 2.37 | C | 26-121 | [»] | |
5VKH | X-ray | 2.25 | C | 22-124 | [»] | |
6NFU | X-ray | 2.09 | C | 22-124 | [»] | |
6NFV | X-ray | 2.13 | C | 22-124 | [»] | |
6PA0 | X-ray | 2.05 | C | 22-124 | [»] | |
6W0A | X-ray | 3.24 | C | 28-120 | [»] | |
6W0B | X-ray | 3.60 | C | 28-120 | [»] | |
6W0C | X-ray | 3.56 | C | 28-120 | [»] | |
6W0D | X-ray | 3.64 | C | 28-120 | [»] | |
6W0E | X-ray | 3.51 | C | 28-120 | [»] | |
6W0F | X-ray | 2.40 | C | 22-124 | [»] | |
6W0G | X-ray | 2.60 | C | 22-124 | [»] | |
6W0H | X-ray | 2.60 | C | 22-124 | [»] | |
6W0I | X-ray | 2.33 | C | 22-124 | [»] | |
6W0J | X-ray | 2.50 | C | 22-124 | [»] | |
7M2I | X-ray | 2.69 | C | 26-116 | [»] | |
7M2J | X-ray | 3.20 | C | 26-116 | [»] | |
7RP0 | X-ray | 2.48 | C | 22-124 | [»] | |
AlphaFoldDBi | P0A334 | |||||
BMRBi | P0A334 | |||||
PCDDBi | P0A334 | |||||
SMRi | P0A334 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-29626N |
MINTi | P0A334 |
Chemistry databases
DrugBanki | DB07416, (2S)-2-(BUTYRYLOXY)-3-HYDROXYPROPYL NONANOATE DB01851, Tetrabutylammonium Ion DB08837, Tetraethylammonium |
Protein family/group databases
TCDBi | 1.A.1.1.1, the voltage-gated ion channel (vic) superfamily |
Protocols and materials databases
ABCDi | P0A334, 3 sequenced antibodies |
Miscellaneous databases
EvolutionaryTracei | P0A334 |
Family and domain databases
InterProi | View protein in InterPro IPR013099, K_chnl_dom IPR028325, VG_K_chnl |
PANTHERi | PTHR11537, PTHR11537, 1 hit |
Pfami | View protein in Pfam PF07885, Ion_trans_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KCSA_STRLI | |
Accessioni | P0A334Primary (citable) accession number: P0A334 Secondary accession number(s): Q54397 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | March 15, 2005 | |
Last modified: | May 25, 2022 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families