UniProtKB - P0A334 (KCSA_STRLI)

BLAST

>sp|P0A334|KCSA_STRLI pH-gated potassium channel KcsA OS=Streptomyces lividans OX=1916 GN=kcsA PE=1 SV=1
MPPMLSGLLARLVKLLLGRHGSALHWRAAGAATVLLVIVLLAGSYLAVLAERGAPGAQLI
TYPRALWWSVETATTVGYGDLYPVTLWGRLVAVVVMVAGITSFGLVTAALATWFVGREQE
RRGHFVRHSEKAAEEAYTRTTRALHERFDRLERMLDDNRR

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History
Entry version 75 (28 Feb 2018)
Sequence version 1 (15 Mar 2005)
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Protein

pH-gated potassium channel KcsA

Gene

kcsA

Organism

Streptomyces lividans

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K⁺ > Rb⁺ > NH4⁺ >> Na⁺ > Li⁺.1 Publication

Miscellaneous

The amino acids 62-79 are situated in the membrane and are important for channel structure and properties.

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ion channel, Ligand-gated ion channel
Biological process	Ion transport, Transport

Protein family/group databases

TCDBⁱ	1.A.1.1.1 the voltage-gated ion channel (vic) superfamily

TCDBⁱ

1.A.1.1.1 the voltage-gated ion channel (vic) superfamily

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: pH-gated potassium channel KcsA Alternative name(s): Streptomyces lividans K+ channel Short name: SKC1
Gene namesⁱ	Name:kcsA Synonyms:skc1
Organismⁱ	Streptomyces lividans
Taxonomic identifierⁱ	1916 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Actinobacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces

Subcellular locationⁱ

Cell membrane ; Multi-pass membrane protein

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 27	CytoplasmicAdd BLAST	27
Transmembraneⁱ	28 – 50	HelicalAdd BLAST	23
Topological domainⁱ	51 – 61	ExtracellularAdd BLAST	11
Intramembraneⁱ	62 – 72	Helical; Pore-formingAdd BLAST	11
Intramembraneⁱ	73 – 80	Pore-forming	8
Topological domainⁱ	81 – 87	Extracellular	7
Transmembraneⁱ	88 – 111	HelicalAdd BLAST	24
Topological domainⁱ	112 – 160	CytoplasmicAdd BLAST	49

GO - Cellular componentⁱ

voltage-gated potassium channel complex Source: InterPro

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell membrane, Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Cells grow slower and to lower myceliar densities.1 Publication

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	71	E → A: Prevents channel inactivation. 1 Publication		1

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB07416 (2S)-2-(BUTYRYLOXY)-3-HYDROXYPROPYL NONANOATE DB01851 Tetrabutylammonium Ion DB08837 Tetraethylammonium

DrugBankⁱ

DB07416 (2S)-2-(BUTYRYLOXY)-3-HYDROXYPROPYL NONANOATE
DB01851 Tetrabutylammonium Ion
DB08837 Tetraethylammonium

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000054102	1 – 160	pH-gated potassium channel KcsAAdd BLAST		160

Interactionⁱ

Subunit structureⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
itself		37	EBI-7262059,EBI-7262059

With

Entry

#Exp.

IntAct

Notes

itself

EBI-7262059,EBI-7262059

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

Database of interacting proteins More...DIPⁱ	DIP-29626N
Molecular INTeraction database More...MINTⁱ	P0A334

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	25 – 51	Combined sources	27
Beta strandⁱ	52 – 55	Combined sources	4
Helixⁱ	62 – 73	Combined sources	12
Beta strandⁱ	79 – 81	Combined sources	3
Helixⁱ	86 – 120	Combined sources	35
Turnⁱ	121 – 123	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	P0A334
SMRⁱ	P0A334
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P0A334

EvolutionaryTraceⁱ

P0A334

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	75 – 80	Selectivity filter		6

Domainⁱ

The cytoplasmic C-terminus is involved in the gating mechanism.

Sequence similaritiesⁱ

Belongs to the potassium channel family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Family and domain databases

InterProⁱ	View protein in InterPro IPR013099 K_chnl_dom IPR028325 VG_K_chnl
PANTHERⁱ	PTHR11537 PTHR11537, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07885 Ion_trans_2, 1 hit
PRINTSⁱ	PR00169 KCHANNEL

Sequenceⁱ

Sequence statusⁱ: Complete.

P0A334-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPPMLSGLLA RLVKLLLGRH GSALHWRAAG AATVLLVIVL LAGSYLAVLA 
        60         70         80         90        100
ERGAPGAQLI TYPRALWWSV ETATTVGYGD LYPVTLWGRL VAVVVMVAGI 
       110        120        130        140        150
TSFGLVTAAL ATWFVGREQE RRGHFVRHSE KAAEEAYTRT TRALHERFDR 
       160
LERMLDDNRR

Length:160

Mass (Da):17,694

Last modified:March 15, 2005 - v1

Checksum:ⁱDEBD9E64384BF40C

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z37969 Genomic DNA Translation: CAA86025.1
PIRⁱ	S60172

Protein	Similar proteins		Species	Score	Length	Source
P0A334	pH-gated potassium channel KcsA	)	STRCO		160	UniRef100_P0A333
Voltage-gated potassium channel		Streptomyces lividans TK24		157
Voltage-gated potassium channel		Streptomyces sp. 2114.2		157
UPI00019A9F18		STRLI		139
UPI00080A7F4E		STRLI		96

Protein	Similar proteins		Species	Score	Length	Source
P0A334	pH-gated potassium channel KcsA	)	STRCO		160	UniRef90_P0A333
Voltage-gated potassium channel		Streptomyces lividans TK24		157
Voltage-gated potassium channel		Streptomyces sp. 2114.2		157
pH-gated potassium channel KcsA		Streptomyces sp. 111WW2		157
UPI00019A9F18		STRLI		139
+5

Protein	Similar proteins		Species	Score	Length	Source
P0A334	pH-gated potassium channel KcsA	)	STRCO		160	UniRef50_P0A333
Voltage-gated potassium channel		Streptomyces lividans TK24		157
Voltage-gated potassium channel		Streptomyces sp. 2114.2		157
pH-gated potassium channel KcsA		Streptomyces sp. 111WW2		157
Voltage-gated potassium channel		Streptomyces sp. WM6386		148
+14

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z37969 Genomic DNA Translation: CAA86025.1
PIRⁱ	S60172

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1BL8	X-ray	3.20	A/B/C/D	23-119	[»]
	1F6G	NMR	-	A/B/C/D	1-160	[»]
	1J95	X-ray	2.80	A/B/C/D	1-125	[»]
	1JQ1	NMR	-	A/B/C/D	86-119	[»]
	1JQ2	NMR	-	A/B/C/D	86-119	[»]
	1JVM	X-ray	2.80	A/B/C/D	1-125	[»]
	1K4C	X-ray	2.00	C	1-124	[»]
	1K4D	X-ray	2.30	C	1-124	[»]
	1R3I	X-ray	2.40	C	1-124	[»]
	1R3J	X-ray	1.90	C	1-124	[»]
	1R3K	X-ray	2.80	C	1-124	[»]
	1R3L	X-ray	2.41	C	1-124	[»]
	1S33	model	-	A/B/C/D	23-119	[»]
	1ZWI	X-ray	2.50	C	22-123	[»]
	2A9H	NMR	-	A/B/C/D	1-132	[»]
	2ATK	X-ray	2.50	C	1-124	[»]
	2BOB	X-ray	2.76	C	1-124	[»]
	2BOC	X-ray	3.01	C	1-124	[»]
	2DWD	X-ray	2.60	C	22-124	[»]
	2DWE	X-ray	2.50	C	22-124	[»]
	2H8P	X-ray	2.25	C	22-78	[»]
				D	80-122	[»]
	2HG5	X-ray	2.75	C	22-78	[»]
				D	80-122	[»]
	2HJF	X-ray	2.90	C	22-124	[»]
	2HVJ	X-ray	2.75	C	1-124	[»]
	2HVK	X-ray	1.90	C	1-124	[»]
	2IH1	X-ray	2.40	C	3-122	[»]
	2IH3	X-ray	1.72	C	3-122	[»]
	2ITC	X-ray	3.20	C	1-124	[»]
	2ITD	X-ray	2.70	C	1-124	[»]
	2JK5	X-ray	2.40	C	1-124	[»]
	2NLJ	X-ray	2.52	C	1-124	[»]
	2P7T	X-ray	2.05	C	22-124	[»]
	2QTO	X-ray	3.20	A/B/C/D	23-119	[»]
	2W0F	X-ray	2.40	C	1-124	[»]
	3EFF	X-ray	3.80	K/L/M/N	22-160	[»]
	3F5W	X-ray	3.30	C	21-124	[»]
	3F7V	X-ray	3.20	C	21-124	[»]
	3F7Y	X-ray	3.40	C	21-124	[»]
	3FB5	X-ray	2.80	C	21-124	[»]
	3FB6	X-ray	3.00	C	21-124	[»]
	3FB7	X-ray	3.30	C	21-124	[»]
	3FB8	X-ray	3.40	C	21-124	[»]
	3GB7	X-ray	2.85	C	1-124	[»]
	3HPL	X-ray	3.20	C	1-124	[»]
	3IFX	Other	3.56	A/B/C/D	1-123	[»]
	3IGA	X-ray	2.75	C	1-124	[»]
	3OGC	X-ray	3.80	C	2-124	[»]
	3OR6	X-ray	2.70	C	22-124	[»]
	3OR7	X-ray	2.30	C	22-124	[»]
	3PJS	X-ray	3.80	K/L/M/N	22-160	[»]
	3STL	X-ray	2.40	C	22-124	[»]
	3STZ	X-ray	2.50	C	23-124	[»]
	4LBE	X-ray	2.75	C	2-124	[»]
	4LCU	X-ray	2.75	C	2-124	[»]
	4MSW	X-ray	2.06	C	22-124	[»]
	4UUJ	X-ray	2.40	C	22-124	[»]
	5E1A	X-ray	3.40	C	4-124	[»]
	5EBL	X-ray	2.30	C	1-125	[»]
	5EBM	X-ray	2.50	C	1-125	[»]
	5EBW	X-ray	2.30	C	1-123	[»]
	5EC1	X-ray	2.75	C	1-125	[»]
5EC2	X-ray	2.30	C	1-125	[»]
5J9P	X-ray	2.85	C	22-117	[»]
5VK6	X-ray	2.25	C	26-121	[»]
5VKE	X-ray	2.37	C	26-121	[»]
5VKH	X-ray	2.25	C	22-124	[»]
ProteinModelPortalⁱ	P0A334
SMRⁱ	P0A334
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

DIPⁱ	DIP-29626N
MINTⁱ	P0A334

Chemistry databases

DrugBankⁱ	DB07416 (2S)-2-(BUTYRYLOXY)-3-HYDROXYPROPYL NONANOATE DB01851 Tetrabutylammonium Ion DB08837 Tetraethylammonium

DrugBankⁱ

DB07416 (2S)-2-(BUTYRYLOXY)-3-HYDROXYPROPYL NONANOATE
DB01851 Tetrabutylammonium Ion
DB08837 Tetraethylammonium

Protein family/group databases

TCDBⁱ	1.A.1.1.1 the voltage-gated ion channel (vic) superfamily

TCDBⁱ

1.A.1.1.1 the voltage-gated ion channel (vic) superfamily

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P0A334

EvolutionaryTraceⁱ

P0A334

Family and domain databases

InterProⁱ	View protein in InterPro IPR013099 K_chnl_dom IPR028325 VG_K_chnl
PANTHERⁱ	PTHR11537 PTHR11537, 1 hit
Pfamⁱ	View protein in Pfam PF07885 Ion_trans_2, 1 hit
PRINTSⁱ	PR00169 KCHANNEL
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KCSA_STRLI
Accessionⁱ	P0A334Primary (citable) accession number: P0A334 Secondary accession number(s): Q54397
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
	Last sequence update:	March 15, 2005
	Last modified:	February 28, 2018
	This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB

UniParc

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Supporting data

UniProtKB - P0A334 (KCSA_STRLI)

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pH-gated potassium channel KcsA

kcsA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Family and domain databases

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

Protocols and materials databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ