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Entry version 81 (18 Sep 2019)
Sequence version 2 (23 Jan 2007)
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Protein

MAPK phosphothreonine lyase

Gene

spvC

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Secreted effector that irreversibly inactivates host MAP kinases by catalyzing the dephosphorylation of the phosphothreonine residue in the pT-X-pY motif in MAPK2/ERK2, MAPK3/ERK1, and p38, via a beta-elimination reaction leading to a dehydrobutyrine residue. Is also able to remove the phosphate group from phospho-JNK in vitro, but JNK may not be a substrate in vivo. Could help suppress localized proinflammatory responses at infection foci in the spleen and liver, and thereby facilitate bacterial growth.4 Publications

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 10.60 sec(-1) with the Erk2 phosphopeptide DHTGFL-pT-E-pY-VATR as substrate.
  1. KM=52.2 µM for the Erk2 phosphopeptide DHTGFL-pT-E-pY-VATR1 Publication
  1. Vmax=22.72 µmol/min/mg enzyme with the Erk2 phosphopeptide DHTGFL-pT-E-pY-VATR as substrate1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei106Proton donor1
Active sitei136Proton acceptor1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLyase
Biological processVirulence

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
SENT99287:PSLT038-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
MAPK phosphothreonine lyase (EC:4.2.3.-)
Alternative name(s):
27.5 kDa virulence protein
Secreted effector protein SpvC
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:spvC
Synonyms:mkaD, vsdD
Ordered Locus Names:PSLT038
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates if the gene coding for the protein originates from the hydrogenosome, the mitochondrion, the nucleomorph, different plastids or a plasmid. The absence of this section means that the gene is located in one of the main chromosomal element(s).<p><a href='/help/encoded_on' target='_top'>More...</a></p>Encoded oniPlasmid pSLT3 Publications
Plasmid pEX1022 Publications
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri99287 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001014 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Plasmid pSLT

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

  • Secreted 1 Publication
  • Note: Can be secreted in vitro by either the SPI-1 or SPI-2 type III secretion systems (T3SS). Translocation of the protein into the cytosol of infected macrophages by intracellular bacteria is dependent on the SPI-2 T3SS. Translocated SpvC proteins appear to be distributed evenly in the cytoplasm of infected cells, and neither colocalize with the SCV membrane nor accumulate in the nucleus of infected cells.

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Strains lacking this gene are attenuated for systemic virulence in mice.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi86F → D: Marked decrease in enzymatic activity. 1 Publication1
Mutagenesisi90R → E: Slight decrease in enzymatic activity. 1 Publication1
Mutagenesisi100F → E: Marked decrease in enzymatic activity. 2 Publications1
Mutagenesisi100F → L: Loss of enzymatic activity. 2 Publications1
Mutagenesisi104K → A or R: Loss of enzymatic activity. 2 Publications1
Mutagenesisi106H → A: Marked decrease in enzymatic activity. 2 Publications1
Mutagenesisi106H → K: 7-fold decrease in enzymatic activity, but no effect on substrate affinity. 2 Publications1
Mutagenesisi106H → N: Loss of enzymatic activity. 2 Publications1
Mutagenesisi134K → A: 2-fold decrease in enzymatic activity. 2 Publications1
Mutagenesisi134K → E: Slight decrease in enzymatic activity. 2 Publications1
Mutagenesisi134K → R: No effect on enzymatic activity. 2 Publications1
Mutagenesisi136K → A or R: Loss of enzymatic activity. 2 Publications1
Mutagenesisi148R → A: Marked decrease in enzymatic activity. 2 Publications1
Mutagenesisi148R → Q: Loss of enzymatic activity. 2 Publications1
Mutagenesisi149V → D: Loss of enzymatic activity. 1 Publication1
Mutagenesisi158Y → E: Marked decrease in enzymatic activity. 2 Publications1
Mutagenesisi158Y → F: 20-fold decrease in enzymatic activity, but no effect on substrate affinity. 2 Publications1
Mutagenesisi160K → A: More than 5-fold decrease in substrate affinity. 2 Publications1
Mutagenesisi160K → E: Slight decrease in enzymatic activity. 2 Publications1
Mutagenesisi160K → R: 2-fold decrease in enzymatic activity, but no effect on substrate affinity. 2 Publications1
Mutagenesisi201D → N: 47-fold decrease in enzymatic activity, but no effect on substrate affinity. 1 Publication1
Mutagenesisi213R → A or Q: Loss of enzymatic activity. 2 Publications1
Mutagenesisi215E → A: Nearly no decrease in enzymatic activity. 1 Publication1
Mutagenesisi220R → A: Marked decrease in enzymatic activity. 2 Publications1
Mutagenesisi220R → Q: Loss of enzymatic activity. 2 Publications1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemoved1 Publication
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002216682 – 241MAPK phosphothreonine lyaseAdd BLAST240

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
P0A2M9

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Database of interacting proteins

More...
DIPi
DIP-46403N

Protein interaction database and analysis system

More...
IntActi
P0A2M9, 1 interactor

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1241
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P0A2M9

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P0A2M9

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the phosphothreonine lyase family.Curated

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000028458

KEGG Orthology (KO)

More...
KOi
K13450

Identification of Orthologs from Complete Genome Data

More...
OMAi
FTLYVKP

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.2430.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003519 OspF/SpvC
IPR038498 OspF/SpvC_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03536 VRP3, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR01342 SALVRPPROT

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P0A2M9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPINRPNLNL NIPPLNIVAA YDGAEIPSTN KHLKNNFNSL HNQMRKMPVS
60 70 80 90 100
HFKEALDVPD YSGMRQSGFF AMSQGFQLNN HGYDVFIHAR RESPQSQGKF
110 120 130 140 150
AGDKFHISVL RDMVPQAFQA LSGLLFSEDS PVDKWKVTDM EKVVQQARVS
160 170 180 190 200
LGAQFTLYIK PDQENSQYSA SFLHKTRQFI ECLESRLSEN GVISGQCPES
210 220 230 240
DVHPENWKYL SYRNELRSGR DGGEMQRQAL REEPFYRLMT E
Length:241
Mass (Da):27,646
Last modified:January 23, 2007 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i77625271D78F8814
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M34355 Genomic DNA Translation: AAA27162.1
AE006471 Genomic DNA Translation: AAL23529.1

Protein sequence database of the Protein Information Resource

More...
PIRi
JQ0747

NCBI Reference Sequences

More...
RefSeqi
NP_490528.1, NC_003277.2
WP_001122242.1, NC_003277.2

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAL23529; AAL23529; PSLT038

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1256201
40104905

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
stm:PSLT038

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|99287.12.peg.4889

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34355 Genomic DNA Translation: AAA27162.1
AE006471 Genomic DNA Translation: AAL23529.1
PIRiJQ0747
RefSeqiNP_490528.1, NC_003277.2
WP_001122242.1, NC_003277.2

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P1WX-ray2.30A1-241[»]
2Q8YX-ray2.00A1-241[»]
2Z8MX-ray2.00A/B1-241[»]
2Z8NX-ray1.80A/B1-241[»]
2Z8OX-ray2.40A/B1-241[»]
2Z8PX-ray1.80A1-241[»]
4H43X-ray2.30A/B1-241[»]
4HAHX-ray1.80A/B1-241[»]
SMRiP0A2M9
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

DIPiDIP-46403N
IntActiP0A2M9, 1 interactor

Proteomic databases

PRIDEiP0A2M9

Genome annotation databases

EnsemblBacteriaiAAL23529; AAL23529; PSLT038
GeneIDi1256201
40104905
KEGGistm:PSLT038
PATRICifig|99287.12.peg.4889

Phylogenomic databases

HOGENOMiHOG000028458
KOiK13450
OMAiFTLYVKP

Enzyme and pathway databases

BioCyciSENT99287:PSLT038-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0A2M9

Protein Ontology

More...
PROi
PR:P0A2M9

Family and domain databases

Gene3Di3.30.2430.10, 1 hit
InterProiView protein in InterPro
IPR003519 OspF/SpvC
IPR038498 OspF/SpvC_sf
PfamiView protein in Pfam
PF03536 VRP3, 1 hit
PRINTSiPR01342 SALVRPPROT

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSPVC_SALTY
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0A2M9
Secondary accession number(s): P21456
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: January 23, 2007
Last modified: September 18, 2019
This is version 81 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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