UniProtKB - P0A283 (PTGA_SALTY)
PTS system glucose-specific EIIA component
crr
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of PtsG and Crr is involved in glucose transport (PubMed:6754734, PubMed:6292227).
It can also phosphorylate mannose, methyl alpha-glucoside and 2-deoxy-glucose (PubMed:6292227).
The non-phosphorylated EIII-Glc is an inhibitor for uptake of certain sugars such as maltose, melibiose, lactose, and glycerol. Phosphorylated EIII-Glc, however, may be an activator for adenylate cyclase (PubMed:789369).
1 Publication3 PublicationsMiscellaneous
Cofactori
Kineticsi
- KM=3.4 µM for methyl alpha-glucoside (with phospho-IIIGlc Slow)1 Publication
- KM=6 µM for methyl alpha-glucoside1 Publication
- KM=10 µM for glucose1 Publication
- KM=40 µM for mannose1 Publication
- KM=200 µM for 2-deoxy-glucose1 Publication
- Vmax=126 µmol/min/mg enzyme with methyl alpha-glucoside as substrate1 Publication
- Vmax=126 µmol/min/mg enzyme with glucose as substrate1 Publication
- Vmax=10 µmol/min/mg enzyme with mannose as substrate1 Publication
- Vmax=10 µmol/min/mg enzyme with 2-deoxy-glucose as substrate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 76 | Zinc; shared with glycerol kinaseBy similarity | 1 | |
Sitei | 76 | Important for phospho-donor activityBy similarity | 1 | |
Active sitei | 91 | Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotationBy similarity | 1 | |
Metal bindingi | 91 | Zinc; shared with glycerol kinaseBy similarity | 1 |
GO - Molecular functioni
- kinase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: PTS system glucose-specific EIIA component1 PublicationAlternative name(s): EIIA-Glc1 Publication EIII-Glc1 Publication Glucose-specific phosphotransferase enzyme IIA component1 Publication |
Gene namesi | Name:crr Ordered Locus Names:STM2433 |
Organismi | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
Taxonomic identifieri | 99287 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000186535 | 2 – 169 | PTS system glucose-specific EIIA componentAdd BLAST | 168 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 91 | Phosphohistidine; by HPrBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P0A283 |
Interactioni
Subunit structurei
Heterodimer with glycerol kinase (glpk).
By similarityProtein-protein interaction databases
IntActi | P0A283, 1 interactor |
STRINGi | 99287.STM2433 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 143 | PTS EIIA type-1PROSITE-ProRule annotationAdd BLAST | 105 |
Domaini
Phylogenomic databases
HOGENOMi | CLU_012312_5_1_6 |
OMAi | HTKHAVG |
PhylomeDBi | P0A283 |
Family and domain databases
Gene3Di | 2.70.70.10, 1 hit |
InterProi | View protein in InterPro IPR011055, Dup_hybrid_motif IPR001127, PTS_EIIA_1_perm |
Pfami | View protein in Pfam PF00358, PTS_EIIA_1, 1 hit |
SUPFAMi | SSF51261, SSF51261, 1 hit |
TIGRFAMsi | TIGR00830, PTBA, 1 hit |
PROSITEi | View protein in PROSITE PS51093, PTS_EIIA_TYPE_1, 1 hit PS00371, PTS_EIIA_TYPE_1_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGLFDKLKSL VSDDKKDTGT IEIVAPLSGE IVNIEDVPDV VFAEKIVGDG
60 70 80 90 100
IAIKPTGNKM VAPVDGTIGK IFETNHAFSI ESDSGIELFV HFGIDTVELK
110 120 130 140 150
GEGFKRIAEE GQRVKVGDPV IEFDLPLLEE KAKSTLTPVV ISNMDEIKEL
160
IKLSGSVTVG ETPVIRIKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05210 Genomic DNA Translation: CAA28837.1 AE006468 Genomic DNA Translation: AAL21327.1 |
PIRi | A03405, WQEB3T |
RefSeqi | NP_461368.1, NC_003197.2 WP_000522253.1, NC_003197.2 |
Genome annotation databases
EnsemblBacteriai | AAL21327; AAL21327; STM2433 |
GeneIDi | 1253955 64336303 67516162 |
KEGGi | stm:STM2433 |
PATRICi | fig|99287.12.peg.2571 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05210 Genomic DNA Translation: CAA28837.1 AE006468 Genomic DNA Translation: AAL21327.1 |
PIRi | A03405, WQEB3T |
RefSeqi | NP_461368.1, NC_003197.2 WP_000522253.1, NC_003197.2 |
3D structure databases
BMRBi | P0A283 |
SMRi | P0A283 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P0A283, 1 interactor |
STRINGi | 99287.STM2433 |
Proteomic databases
PaxDbi | P0A283 |
Genome annotation databases
EnsemblBacteriai | AAL21327; AAL21327; STM2433 |
GeneIDi | 1253955 64336303 67516162 |
KEGGi | stm:STM2433 |
PATRICi | fig|99287.12.peg.2571 |
Phylogenomic databases
HOGENOMi | CLU_012312_5_1_6 |
OMAi | HTKHAVG |
PhylomeDBi | P0A283 |
Miscellaneous databases
PROi | PR:P0A283 |
Family and domain databases
Gene3Di | 2.70.70.10, 1 hit |
InterProi | View protein in InterPro IPR011055, Dup_hybrid_motif IPR001127, PTS_EIIA_1_perm |
Pfami | View protein in Pfam PF00358, PTS_EIIA_1, 1 hit |
SUPFAMi | SSF51261, SSF51261, 1 hit |
TIGRFAMsi | TIGR00830, PTBA, 1 hit |
PROSITEi | View protein in PROSITE PS51093, PTS_EIIA_TYPE_1, 1 hit PS00371, PTS_EIIA_TYPE_1_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PTGA_SALTY | |
Accessioni | P0A283Primary (citable) accession number: P0A283 Secondary accession number(s): P02908 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 94 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |