UniProtKB - P0A251 (AHPC_SALTY)
Protein
Alkyl hydroperoxide reductase C
Gene
ahpC
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.2 Publications
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by AhpF.1 Publication1 Publication
Catalytic activityi
- EC:1.11.1.261 Publication
Kineticsi
kcat is 55.1 sec(-1) with H2O2 as substrate.1 Publication
- KM=1.4 µM for H2O21 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 47 | Cysteine sulfenic acid (-SOH) intermediate1 Publication | 1 |
GO - Molecular functioni
- alkylhydroperoxide reductase activity Source: UniProtKB-EC
- peroxidase activity Source: UniProtKB-KW
- peroxiredoxin activity Source: InterPro
GO - Biological processi
- cell redox homeostasis Source: GO_Central
- response to oxidative stress Source: InterPro
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Enzyme and pathway databases
BRENDAi | 1.11.1.15, 5542 |
Protein family/group databases
PeroxiBasei | 4829, SetypAhpC |
Names & Taxonomyi
Protein namesi | Recommended name: Alkyl hydroperoxide reductase C1 Publication (EC:1.11.1.261 Publication)Alternative name(s): Alkyl hydroperoxide reductase protein C221 Publication Peroxiredoxin Thioredoxin peroxidase |
Gene namesi | Name:ahpC1 Publication Ordered Locus Names:STM0608 |
Organismi | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
Taxonomic identifieri | 99287 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000135120 | 2 – 187 | Alkyl hydroperoxide reductase CAdd BLAST | 186 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 47 | Interchain (with C-166); in linked formCombined sources1 Publication | ||
Disulfide bondi | 166 | Interchain (with C-47); in linked formCombined sources1 Publication |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | P0A251 |
Interactioni
Subunit structurei
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0A251 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0A251 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 157 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 156 |
Sequence similaritiesi
Keywords - Domaini
Redox-active centerPhylogenomic databases
HOGENOMi | CLU_042529_21_3_6 |
OMAi | FWYPKDF |
PhylomeDBi | P0A251 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR017559, AhpC IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10681:SF121, PTHR10681:SF121, 1 hit |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR03137, AhpC, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A251-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLINTKIKP FKNQAFKNGE FIEVTEKDTE GRWSVFFFYP ADFTFVCPTE
60 70 80 90 100
LGDVADHYEE LQKLGVDVYS VSTDTHFTHK AWHSSSETIA KIKYAMIGDP
110 120 130 140 150
TGALTRNFDN MREDEGLADR ATFVVDPQGI IQAIEVTAEG IGRDASDLLR
160 170 180
KIKAAQYVAA HPGEVCPAKW KEGEATLAPS LDLVGKI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | S → G AA sequence (PubMed:2643600).Curated | 1 | |
Sequence conflicti | 5 | N → D AA sequence (PubMed:2643600).Curated | 1 | |
Sequence conflicti | 14 | Q → N AA sequence (PubMed:2643600).Curated | 1 | |
Sequence conflicti | 17 | K → H AA sequence (PubMed:2643600).Curated | 1 | |
Sequence conflicti | 20 | E → H AA sequence (PubMed:2643600).Curated | 1 | |
Sequence conflicti | 23 | E → S AA sequence (PubMed:2643600).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05478 Unassigned DNA Translation: AAA16431.1 AE006468 Genomic DNA Translation: AAL19559.1 |
PIRi | A35441 |
RefSeqi | NP_459600.1, NC_003197.2 WP_000052802.1, NC_003197.2 |
Genome annotation databases
EnsemblBacteriai | AAL19559; AAL19559; STM0608 |
GeneIDi | 1252128 57493531 |
KEGGi | stm:STM0608 |
PATRICi | fig|99287.12.peg.640 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05478 Unassigned DNA Translation: AAA16431.1 AE006468 Genomic DNA Translation: AAL19559.1 |
PIRi | A35441 |
RefSeqi | NP_459600.1, NC_003197.2 WP_000052802.1, NC_003197.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1N8J | X-ray | 2.17 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T | 2-187 | [»] | |
1YEP | X-ray | 2.50 | A/B/C/D/E | 2-187 | [»] | |
1YEX | X-ray | 2.30 | A/B/C/D/E | 2-187 | [»] | |
1YF0 | X-ray | 2.50 | A/B/C/D/E | 2-187 | [»] | |
1YF1 | X-ray | 2.60 | A/B/C/D/E/F/G/H/I/J | 2-187 | [»] | |
3EMP | X-ray | 4.00 | A/B/C/D/E | 2-187 | [»] | |
4MA9 | X-ray | 1.82 | A/B/C/D/E | 2-187 | [»] | |
4MAB | X-ray | 1.90 | A/B/C/D/E | 2-187 | [»] | |
4XRA | X-ray | 1.75 | A/B/C/D/E | 2-187 | [»] | |
4XRD | X-ray | 2.30 | A/B/C/D/E | 2-187 | [»] | |
4XS1 | X-ray | 2.10 | A/B/C/D/E | 2-187 | [»] | |
4XS4 | X-ray | 2.65 | A/B/C/D/E | 2-187 | [»] | |
4XS6 | X-ray | 3.35 | A/B/C/D/E | 2-187 | [»] | |
4XTS | X-ray | 2.70 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T | 2-187 | [»] | |
5UKA | X-ray | 1.90 | A/B/C/D/E | 2-187 | [»] | |
SMRi | P0A251 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
PeroxiBasei | 4829, SetypAhpC |
Proteomic databases
PaxDbi | P0A251 |
Genome annotation databases
EnsemblBacteriai | AAL19559; AAL19559; STM0608 |
GeneIDi | 1252128 57493531 |
KEGGi | stm:STM0608 |
PATRICi | fig|99287.12.peg.640 |
Phylogenomic databases
HOGENOMi | CLU_042529_21_3_6 |
OMAi | FWYPKDF |
PhylomeDBi | P0A251 |
Enzyme and pathway databases
BRENDAi | 1.11.1.15, 5542 |
Miscellaneous databases
EvolutionaryTracei | P0A251 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR017559, AhpC IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10681:SF121, PTHR10681:SF121, 1 hit |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR03137, AhpC, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AHPC_SALTY | |
Accessioni | P0A251Primary (citable) accession number: P0A251 Secondary accession number(s): P19479 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 115 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families