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Protein

Methionine aminopeptidase

Gene

map

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.UniRule annotation1 Publication

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79SubstrateUniRule annotation1
Metal bindingi97Divalent metal cation 1UniRule annotation1
Metal bindingi108Divalent metal cation 1UniRule annotation1
Metal bindingi108Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi171Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei178SubstrateUniRule annotation1
Metal bindingi204Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi235Divalent metal cation 1UniRule annotation1
Metal bindingi235Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-217-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:mapUniRule annotation
Synonyms:pepM
Ordered Locus Names:STM0215
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001489522 – 264Methionine aminopeptidaseAdd BLAST263

Proteomic databases

PaxDbiP0A1X6
PRIDEiP0A1X6

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi99287.STM0215

Structurei

3D structure databases

ProteinModelPortaliP0A1X6
SMRiP0A1X6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA1 Bacteria
COG0024 LUCA
HOGENOMiHOG000030427
KOiK01265
OMAiGDHAYTF
PhylomeDBiP0A1X6

Family and domain databases

CDDicd01086 MetAP1, 1 hit
HAMAPiMF_01974 MetAP_1, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002467 Pept_M24A_MAP1
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00500 met_pdase_I, 1 hit
PROSITEiView protein in PROSITE
PS00680 MAP_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A1X6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAISIKTSED IEKMRVAGRL AAEVLEMIEP YIKPGVTTGE LDRICNDYIV
60 70 80 90 100
NEQHAISACL GYHGYPKSVC ISINEVVCHG IPDDAKHLKD GDIVNIDVTV
110 120 130 140 150
IKDEFHGDTS KMFIVGKPTI LGERLCRVTQ ESLYLGIKMV KPGIRLRTIG
160 170 180 190 200
AAIQKYAEGE GFSVVREYCG HGIGRGFHEE PQVLHYDADD GGVVLQPGMT
210 220 230 240 250
FTIEPMLNAG DYRIRTMKDG WTVKTKDRSL SAQYEHTIVV TENGCEILTL
260
RKDDTIPAII THDE
Length:264
Mass (Da):29,292
Last modified:January 23, 2007 - v2
Checksum:i8563A54BBE98A860
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136 – 138GIK → ALR AA sequence (PubMed:2651123).Curated3
Sequence conflicti145R → N AA sequence (PubMed:2651123).Curated1
Sequence conflicti148T → E AA sequence (PubMed:2651123).Curated1
Sequence conflicti159G → A AA sequence (PubMed:2651123).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55778 Genomic DNA Translation: CAA39298.1
AE006468 Genomic DNA Translation: AAL19179.1
PIRiS12027
RefSeqiNP_459220.1, NC_003197.2
WP_001018214.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL19179; AAL19179; STM0215
GeneIDi1251733
KEGGistm:STM0215
PATRICifig|99287.12.peg.228

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55778 Genomic DNA Translation: CAA39298.1
AE006468 Genomic DNA Translation: AAL19179.1
PIRiS12027
RefSeqiNP_459220.1, NC_003197.2
WP_001018214.1, NC_003197.2

3D structure databases

ProteinModelPortaliP0A1X6
SMRiP0A1X6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0215

Proteomic databases

PaxDbiP0A1X6
PRIDEiP0A1X6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19179; AAL19179; STM0215
GeneIDi1251733
KEGGistm:STM0215
PATRICifig|99287.12.peg.228

Phylogenomic databases

eggNOGiENOG4105CA1 Bacteria
COG0024 LUCA
HOGENOMiHOG000030427
KOiK01265
OMAiGDHAYTF
PhylomeDBiP0A1X6

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-217-MONOMER

Family and domain databases

CDDicd01086 MetAP1, 1 hit
HAMAPiMF_01974 MetAP_1, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002467 Pept_M24A_MAP1
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00500 met_pdase_I, 1 hit
PROSITEiView protein in PROSITE
PS00680 MAP_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMAP1_SALTY
AccessioniPrimary (citable) accession number: P0A1X6
Secondary accession number(s): P10882
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 23, 2007
Last modified: October 10, 2018
This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
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Main funding by: National Institutes of Health

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