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Protein

RNA polymerase sigma-54 factor

Gene

rpoN

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the xylCAB operon and the xylS gene. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for receipt of the melting signal from the remotely bound activator protein XylR for the expression of the xylCAB operon and xylS.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi386 – 405H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Sigma factor, Transferase
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-1026-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-54 factor
Gene namesi
Name:rpoN
Synonyms:ntrA
Ordered Locus Names:PP_0952
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  • protein-DNA complex Source: CollecTF

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002055351 – 497RNA polymerase sigma-54 factorAdd BLAST497

Proteomic databases

PRIDEiP0A171

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-8302876,EBI-8302876

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

MINTiP0A171
STRINGi160488.PP_0952

Structurei

3D structure databases

ProteinModelPortaliP0A171
SMRiP0A171
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi474 – 482RPON box9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 35Gln-richAdd BLAST25

Sequence similaritiesi

Belongs to the sigma-54 factor family.Curated

Phylogenomic databases

eggNOGiENOG4105DTW Bacteria
COG1508 LUCA
HOGENOMiHOG000071905
KOiK03092
OMAiVTTQKFM

Family and domain databases

Gene3Di1.10.10.1330, 1 hit
InterProiView protein in InterPro
IPR000394 RNA_pol_sigma_54
IPR007046 RNA_pol_sigma_54_core-bd
IPR007634 RNA_pol_sigma_54_DNA-bd
IPR038709 RpoN_core-bd_sf
PANTHERiPTHR32248 PTHR32248, 1 hit
PfamiView protein in Pfam
PF00309 Sigma54_AID, 1 hit
PF04963 Sigma54_CBD, 1 hit
PF04552 Sigma54_DBD, 1 hit
PIRSFiPIRSF000774 RpoN, 1 hit
PRINTSiPR00045 SIGMA54FCT
TIGRFAMsiTIGR02395 rpoN_sigma, 1 hit
PROSITEiView protein in PROSITE
PS00717 SIGMA54_1, 1 hit
PS00718 SIGMA54_2, 1 hit
PS50044 SIGMA54_3, 1 hit

Sequencei

Sequence statusi: Complete.

P0A171-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ
60 70 80 90 100
EDGEDFDNSD PMADNAENKP AAEVQDNSFQ ESTVSADNLE DGEWSERIPN
110 120 130 140 150
ELPVDTAWED IYQTSASSLP SNDDDEWDFT TRTSAGESLQ SHLLWQLNLA
160 170 180 190 200
PMSDTDRLIA VTLIDSINGQ GYLEDTLEEI CAGFDPELDI ELDEVEAVLH
210 220 230 240 250
RIQQFEPAGV GARNLGECLL LQLRQLPATT PWMTEAKRLV TDFIDLLGSR
260 270 280 290 300
DYSQLMRRMK IKEDELRQVI ELVQSLNPRP GSQIESSEPE YVVPDVIVRK
310 320 330 340 350
DSDRWLVELN QEAIPRLRVN PQYAGFVRRA DTSADNTFMR NQLQEARWFI
360 370 380 390 400
KSLQSRNETL MKVATQIVEH QRGFLDHGDE AMKPLVLHDI AEAVGMHEST
410 420 430 440 450
ISRVTTQKYM HTPRGIYELK YFFSSHVSTS EGGECSSTAI RAIIKKLVAA
460 470 480 490
ENQKKPLSDS KIAGLLEAQG IQVARRTVAK YRESLGIAPS SERKRLM
Length:497
Mass (Da):56,215
Last modified:March 1, 2005 - v1
Checksum:i921E788B48B5B2F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181C → S in CAA34494 (PubMed:2602128).Curated1
Sequence conflicti366Q → R in CAA34494 (PubMed:2602128).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16474 Genomic DNA Translation: CAA34494.1
AE015451 Genomic DNA Translation: AAN66577.1
PIRiT01753
RefSeqiNP_743113.1, NC_002947.4
WP_003255133.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN66577; AAN66577; PP_0952
GeneIDi1044867
KEGGippu:PP_0952
PATRICifig|160488.4.peg.1013

Similar proteinsi

Entry informationi

Entry nameiRP54_PSEPK
AccessioniPrimary (citable) accession number: P0A171
Secondary accession number(s): P15591
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: June 20, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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