Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

LexA repressor 1

Gene

lexA1

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.UniRule annotation

Catalytic activityi

Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei123For autocatalytic cleavage activityUniRule annotation1
Active sitei160For autocatalytic cleavage activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi28 – 48H-T-H motifUniRule annotationAdd BLAST21

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Hydrolase, Repressor
Biological processDNA damage, DNA repair, DNA replication, SOS response, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-2284-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
LexA repressor 1UniRule annotation (EC:3.4.21.88UniRule annotation)
Gene namesi
Name:lexA1UniRule annotation
Synonyms:lexA, lexA-1
Ordered Locus Names:PP_2143
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  • protein-DNA complex Source: CollecTF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001700691 – 202LexA repressor 1Add BLAST202

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei88 – 89Cleavage; by autolysisUniRule annotation2

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PRIDEiP0A153

Expressioni

Gene expression databases

CollecTFiEXPREG_00000710

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi160488.PP_2143

Structurei

3D structure databases

ProteinModelPortaliP0A153
SMRiP0A153
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S24 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DS7 Bacteria
COG1974 LUCA
HOGENOMiHOG000232167
KOiK01356
OMAiRQMMQAM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00015 LexA, 1 hit
InterProiView protein in InterPro
IPR006200 LexA
IPR036286 LexA/Signal_pep-like_sf
IPR006199 LexA_DNA-bd_dom
IPR006197 Peptidase_S24_LexA
IPR015927 Peptidase_S24_S26A/B/C
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF01726 LexA_DNA_bind, 1 hit
PF00717 Peptidase_S24, 1 hit
PRINTSiPR00726 LEXASERPTASE
SUPFAMiSSF46785 SSF46785, 1 hit
SSF51306 SSF51306, 1 hit
TIGRFAMsiTIGR00498 lexA, 1 hit

Sequencei

Sequence statusi: Complete.

P0A153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKLTPRQAE ILAFIKRCLE DNGFPPTRAE IAQELGFKSP NAAEEHLKAL
60 70 80 90 100
ARKGAIEMTP GASRGIRIPG LEAKAEEAGL PIIGRVAAGA PILAEQHIEQ
110 120 130 140 150
SCNINPAFFH PQADYLLRVH GMSMKDVGIF DGDLLAVHTC REARNGQIVV
160 170 180 190 200
ARIGDEVTVK RFKREGSKVW LLAENPEFAP IEVDLKEQEL VIEGLSVGVI

RR
Length:202
Mass (Da):22,148
Last modified:March 1, 2005 - v1
Checksum:iEF43C178C97D0679
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN67756.1
RefSeqiNP_744292.1, NC_002947.4
WP_010953131.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN67756; AAN67756; PP_2143
GeneIDi1044998
KEGGippu:PP_2143
PATRICifig|160488.4.peg.2260

Similar proteinsi

Entry informationi

Entry nameiLEXA1_PSEPK
AccessioniPrimary (citable) accession number: P0A153
Secondary accession number(s): P37453, Q9EUU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health