UniProtKB - P0A0B7 (AHPC_STAA8)
Protein
Alkyl hydroperoxide reductase C
Gene
ahpC
Organism
Staphylococcus aureus (strain NCTC 8325 / PS 47)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Is important for survival under desiccation conditions. Not required for virulence although is necessary for nasal colonization.1 Publication
Miscellaneous
In S.aureus, the removal of hydrogen peroxide appears to occur predominately via KatA, with AhpC acting as an alternative.1 Publication
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by AhpF.By similarity
Catalytic activityi
- EC:1.11.1.26By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 49 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- alkylhydroperoxide reductase activity Source: UniProtKB-EC
- thioredoxin peroxidase activity Source: GO_Central
GO - Biological processi
- cell redox homeostasis Source: GO_Central
- response to oxidative stress Source: GO_Central
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Names & Taxonomyi
Protein namesi | Recommended name: Alkyl hydroperoxide reductase C (EC:1.11.1.26By similarity)Alternative name(s): Peroxiredoxin Thioredoxin peroxidase |
Gene namesi | Name:ahpC Ordered Locus Names:SAOUHSC_00365 |
Organismi | Staphylococcus aureus (strain NCTC 8325 / PS 47) |
Taxonomic identifieri | 93061 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm By similarity
GO - Cellular componenti
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000135128 | 2 – 189 | Alkyl hydroperoxide reductase CAdd BLAST | 188 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 49 | Interchain (with C-168); in linked formBy similarity | ||
Disulfide bondi | 168 | Interchain (with C-49); in linked formBy similarity |
Keywords - PTMi
Disulfide bondExpressioni
Inductioni
Induced by iron and osmotic shock. Repressed under metal-depleted growth conditions and by manganese-rich growth conditions. Negatively regulated by the ferric uptake regulator (Fur) and PerR.2 Publications
Interactioni
Subunit structurei
Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer.
By similarityProtein-protein interaction databases
STRINGi | 1280.SAXN108_0431 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 159 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 158 |
Sequence similaritiesi
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | COG0450, Bacteria |
HOGENOMi | CLU_042529_21_3_9 |
OMAi | FWYPKDF |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR017559, AhpC IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10681:SF121, PTHR10681:SF121, 1 hit |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR03137, AhpC, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0A0B7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLINKEILP FTAQAFDPKK DQFKEVTQED LKGSWSVVCF YPADFSFVCP
60 70 80 90 100
TELEDLQNQY EELQKLGVNV FSVSTDTHFV HKAWHDHSDA ISKITYTMIG
110 120 130 140 150
DPSQTITRNF DVLDEATGLA QRGTFIIDPD GVVQASEINA DGIGRDASTL
160 170 180
AHKIKAAQYV RKNPGEVCPA KWEEGAKTLQ PGLDLVGKI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | S → G AA sequence (PubMed:7551034).Curated | 1 | |
Sequence conflicti | 17 | D → DD AA sequence (PubMed:7551034).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92441 Genomic DNA Translation: AAB51151.1 CP000253 Genomic DNA Translation: ABD29531.1 |
PIRi | S52934 |
RefSeqi | WP_000052781.1, NZ_LS483365.1 YP_498954.1, NC_007795.1 |
Genome annotation databases
EnsemblBacteriai | ABD29531; ABD29531; SAOUHSC_00365 |
GeneIDi | 3919784 45573660 |
KEGGi | sao:SAOUHSC_00365 |
PATRICi | fig|93061.5.peg.335 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92441 Genomic DNA Translation: AAB51151.1 CP000253 Genomic DNA Translation: ABD29531.1 |
PIRi | S52934 |
RefSeqi | WP_000052781.1, NZ_LS483365.1 YP_498954.1, NC_007795.1 |
3D structure databases
SMRi | P0A0B7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 1280.SAXN108_0431 |
Genome annotation databases
EnsemblBacteriai | ABD29531; ABD29531; SAOUHSC_00365 |
GeneIDi | 3919784 45573660 |
KEGGi | sao:SAOUHSC_00365 |
PATRICi | fig|93061.5.peg.335 |
Phylogenomic databases
eggNOGi | COG0450, Bacteria |
HOGENOMi | CLU_042529_21_3_9 |
OMAi | FWYPKDF |
Miscellaneous databases
PROi | PR:P0A0B7 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR017559, AhpC IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10681:SF121, PTHR10681:SF121, 1 hit |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR03137, AhpC, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AHPC_STAA8 | |
Accessioni | P0A0B7Primary (citable) accession number: P0A0B7 Secondary accession number(s): Q2G0Z6, Q53647 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | March 1, 2005 | |
Last modified: | December 2, 2020 | |
This is version 93 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families