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Protein

Penicillinase repressor

Gene

blaI

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor that constitutively blocks expression of beta-lactamase. Binds DNA as a dimer (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi7 – 71H-T-H motifBy similarityAdd BLAST65

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processAntibiotic resistance, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillinase repressor
Alternative name(s):
Beta-lactamase repressor protein
Regulatory protein BlaI
Gene namesi
Name:blaI
Synonyms:penI
Encoded oniPlasmid pI2580 Publication
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi101 – 102NF → AA: Abolishes proteolytic cleavage. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000627931 – 126Penicillinase repressorAdd BLAST126

Post-translational modificationi

Upon exposure to beta-lactams, the protease BlaR1 is activated and cleaves BlaI at a single site. This proteolytic cleavage impairs dimerization and abolishes repressor activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei101 – 102Cleavage2

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1126
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 21Combined sources12
Beta strandi22 – 26Combined sources5
Helixi27 – 35Combined sources9
Helixi42 – 54Combined sources13
Beta strandi57 – 63Combined sources7
Beta strandi66 – 71Combined sources6
Helixi75 – 91Combined sources17
Helixi95 – 104Combined sources10
Helixi110 – 123Combined sources14

3D structure databases

ProteinModelPortaliP0A042
SMRiP0A042
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A042

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni74 – 126Important for dimerizationBy similarityAdd BLAST53

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR005650 BlaI_family
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR34134 PTHR34134, 1 hit
PfamiView protein in Pfam
PF03965 Penicillinase_R, 1 hit
PIRSFiPIRSF019455 CopR_AtkY, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit

Sequencei

Sequence statusi: Complete.

P0A042-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANKQVEISM AEWDVMNIIW GKKSVSANEI VVEIQKYKEV SDKTIRTLIT
60 70 80 90 100
RLYKKEIIKR YKSENIYFYS SNIKEDDIKM KTAKTFLNKL YGGDMKSLVL
110 120
NFAKNEELNN KEIEELRDIL NDISKK
Length:126
Mass (Da):14,876
Last modified:February 15, 2005 - v1
Checksum:iE0759E83A5183117
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52734 Genomic DNA Translation: CAA36951.1
M62650 Genomic DNA Translation: AAA26603.1
M92376 Genomic DNA Translation: AAA26605.1
PIRiS11782
RefSeqiNP_878025.1, NC_005054.1
WP_001284656.1, NZ_PYFC01000004.1
YP_003329486.1, NC_013550.1
YP_006937604.1, NC_013319.1
YP_006937749.1, NC_013323.1
YP_006938265.1, NC_013337.1
YP_006938772.1, NC_013352.1
YP_008709797.1, NC_022598.1

Genome annotation databases

GeneIDi13874752
13874901
13875432
13875953
17363238
2598289
29755021
8655738

Similar proteinsi

Entry informationi

Entry nameiBLAI_STAAU
AccessioniPrimary (citable) accession number: P0A042
Secondary accession number(s): P18415
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: July 18, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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