UniProtKB - P09980 (REP_ECOLI)
Protein
ATP-dependent DNA helicase Rep
Gene
rep
Organism
Escherichia coli (strain K12)
Status
Functioni
Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction.UniRule annotation1 Publication
Catalytic activityi
- EC:3.6.4.12UniRule annotation2 Publications
Activity regulationi
Binding to DNA induces dimerization, which is required for DNA helicase activity.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 278 | ATPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 22 – 29 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- 3'-5' DNA helicase activity Source: GO_Central
- 3'-5' exonuclease activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-UniRule
- DNA helicase activity Source: EcoCyc
- single-stranded DNA binding Source: UniProtKB-UniRule
GO - Biological processi
- bacterial-type DNA replication Source: EcoCyc
- DNA unwinding involved in DNA replication Source: EcoCyc
- recombinational repair Source: GO_Central
- response to radiation Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10837-MONOMER MetaCyc:EG10837-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:repUniRule annotation Ordered Locus Names:b3778, JW5604 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000102068 | 1 – 673 | ATP-dependent DNA helicase RepAdd BLAST | 673 |
Proteomic databases
jPOSTi | P09980 |
PaxDbi | P09980 |
PRIDEi | P09980 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation2 PublicationsBinary interactionsi
P09980
With | #Exp. | IntAct |
---|---|---|
dnaB [P0ACB0] | 3 | EBI-6558011,EBI-548978 |
Protein-protein interaction databases
BioGRIDi | 4263317, 69 interactors 852591, 3 interactors |
ComplexPortali | CPX-1953, Replication restart pre-primosome complex priC-rep variant CPX-5911, Replication restart primosome complex, priC-rep variant |
DIPi | DIP-10662N |
IntActi | P09980, 4 interactors |
STRINGi | 511145.b3778 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P09980 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09980 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 280 | UvrD-like helicase ATP-bindingUniRule annotationAdd BLAST | 280 | |
Domaini | 281 – 562 | UvrD-like helicase C-terminalUniRule annotationAdd BLAST | 282 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0210, Bacteria |
HOGENOMi | CLU_004585_5_4_6 |
InParanoidi | P09980 |
PhylomeDBi | P09980 |
Family and domain databases
Gene3Di | 1.10.10.160, 1 hit |
HAMAPi | MF_01920, Helicase_Rep, 1 hit |
InterProi | View protein in InterPro IPR013986, DExx_box_DNA_helicase_dom_sf IPR014017, DNA_helicase_UvrD-like_C IPR000212, DNA_helicase_UvrD/REP IPR005752, Helicase_Rep IPR027417, P-loop_NTPase IPR014016, UvrD-like_ATP-bd IPR034739, UvrD/AddA_N |
PANTHERi | PTHR11070, PTHR11070, 1 hit |
Pfami | View protein in Pfam PF00580, UvrD-helicase, 1 hit PF13361, UvrD_C, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01074, rep, 1 hit |
PROSITEi | View protein in PROSITE PS51198, UVRD_HELICASE_ATP_BIND, 1 hit PS51217, UVRD_HELICASE_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
P09980-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLNPGQQQA VEFVTGPCLV LAGAGSGKTR VITNKIAHLI RGCGYQARHI
60 70 80 90 100
AAVTFTNKAA REMKERVGQT LGRKEARGLM ISTFHTLGLD IIKREYAALG
110 120 130 140 150
MKANFSLFDD TDQLALLKEL TEGLIEDDKV LLQQLISTIS NWKNDLKTPS
160 170 180 190 200
QAAASAIGER DRIFAHCYGL YDAHLKACNV LDFDDLILLP TLLLQRNEEV
210 220 230 240 250
RKRWQNKIRY LLVDEYQDTN TSQYELVKLL VGSRARFTVV GDDDQSIYSW
260 270 280 290 300
RGARPQNLVL LSQDFPALKV IKLEQNYRSS GRILKAANIL IANNPHVFEK
310 320 330 340 350
RLFSELGYGA ELKVLSANNE EHEAERVTGE LIAHHFVNKT QYKDYAILYR
360 370 380 390 400
GNHQSRVFEK FLMQNRIPYK ISGGTSFFSR PEIKDLLAYL RVLTNPDDDS
410 420 430 440 450
AFLRIVNTPK REIGPATLKK LGEWAMTRNK SMFTASFDMG LSQTLSGRGY
460 470 480 490 500
EALTRFTHWL AEIQRLAERE PIAAVRDLIH GMDYESWLYE TSPSPKAAEM
510 520 530 540 550
RMKNVNQLFS WMTEMLEGSE LDEPMTLTQV VTRFTLRDMM ERGESEEELD
560 570 580 590 600
QVQLMTLHAS KGLEFPYVYM VGMEEGFLPH QSSIDEDNID EERRLAYVGI
610 620 630 640 650
TRAQKELTFT LCKERRQYGE LVRPEPSRFL LELPQDDLIW EQERKVVSAE
660 670
ERMQKGQSHL ANLKAMMAAK RGK
Sequence cautioni
The sequence CAA28481 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 73 | R → H in CAA28481 (PubMed:3029683).Curated | 1 | |
Sequence conflicti | 76 | A → Q in CAA28481 (PubMed:3029683).Curated | 1 | |
Sequence conflicti | 163 – 165 | IFA → LLL in CAA28481 (PubMed:3029683).Curated | 3 | |
Sequence conflicti | 196 | R → A in AAA67579 (PubMed:1379743).Curated | 1 | |
Sequence conflicti | 202 – 203 | KR → NG in CAA28481 (PubMed:3029683).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04794 Genomic DNA Translation: CAA28481.1 Frameshift. M87049 Genomic DNA Translation: AAA67579.1 U00096 Genomic DNA Translation: AAT48209.1 AP009048 Genomic DNA Translation: BAE77520.1 M11055 Genomic DNA Translation: AAA24518.1 |
PIRi | E65181, HJECDR |
RefSeqi | WP_001238899.1, NZ_LN832404.1 YP_026251.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48209; AAT48209; b3778 BAE77520; BAE77520; BAE77520 |
GeneIDi | 948292 |
KEGGi | ecj:JW5604 eco:b3778 |
PATRICi | fig|511145.12.peg.3893 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04794 Genomic DNA Translation: CAA28481.1 Frameshift. M87049 Genomic DNA Translation: AAA67579.1 U00096 Genomic DNA Translation: AAT48209.1 AP009048 Genomic DNA Translation: BAE77520.1 M11055 Genomic DNA Translation: AAA24518.1 |
PIRi | E65181, HJECDR |
RefSeqi | WP_001238899.1, NZ_LN832404.1 YP_026251.1, NC_000913.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UAA | X-ray | 3.00 | A/B | 1-673 | [»] | |
SMRi | P09980 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263317, 69 interactors 852591, 3 interactors |
ComplexPortali | CPX-1953, Replication restart pre-primosome complex priC-rep variant CPX-5911, Replication restart primosome complex, priC-rep variant |
DIPi | DIP-10662N |
IntActi | P09980, 4 interactors |
STRINGi | 511145.b3778 |
Proteomic databases
jPOSTi | P09980 |
PaxDbi | P09980 |
PRIDEi | P09980 |
Genome annotation databases
EnsemblBacteriai | AAT48209; AAT48209; b3778 BAE77520; BAE77520; BAE77520 |
GeneIDi | 948292 |
KEGGi | ecj:JW5604 eco:b3778 |
PATRICi | fig|511145.12.peg.3893 |
Organism-specific databases
EchoBASEi | EB0830 |
Phylogenomic databases
eggNOGi | COG0210, Bacteria |
HOGENOMi | CLU_004585_5_4_6 |
InParanoidi | P09980 |
PhylomeDBi | P09980 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10837-MONOMER MetaCyc:EG10837-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P09980 |
PROi | PR:P09980 |
Family and domain databases
Gene3Di | 1.10.10.160, 1 hit |
HAMAPi | MF_01920, Helicase_Rep, 1 hit |
InterProi | View protein in InterPro IPR013986, DExx_box_DNA_helicase_dom_sf IPR014017, DNA_helicase_UvrD-like_C IPR000212, DNA_helicase_UvrD/REP IPR005752, Helicase_Rep IPR027417, P-loop_NTPase IPR014016, UvrD-like_ATP-bd IPR034739, UvrD/AddA_N |
PANTHERi | PTHR11070, PTHR11070, 1 hit |
Pfami | View protein in Pfam PF00580, UvrD-helicase, 1 hit PF13361, UvrD_C, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01074, rep, 1 hit |
PROSITEi | View protein in PROSITE PS51198, UVRD_HELICASE_ATP_BIND, 1 hit PS51217, UVRD_HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | REP_ECOLI | |
Accessioni | P09980Primary (citable) accession number: P09980 Secondary accession number(s): Q2M886, Q6BF05 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | October 11, 2004 | |
Last modified: | April 7, 2021 | |
This is version 169 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families