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Protein

Alpha-amylase 1

Gene

amyA

Organism
Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This amylase is a highly liquefying-type: oligomers appeared at the beginning of incubation, followed by a graded decrease in the amounts of maltotriose, maltose and glucose in prolonged incubation.

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

pH dependencei

Optimum pH is 5.5. Stable above pH 5.5.

Temperature dependencei

Optimum temperature is about 90 degrees Celsius. Highly thermostable. Retains 70% of its maximal activity after heating 1 hour at 90 degrees Celsius, but no decrease in activity was observed within the same time at 80 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei125NucleophileBy similarity1
Active sitei216Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciDTHE309799:G1GCP-719-MONOMER

Protein family/group databases

CAZyiGH57 Glycoside Hydrolase Family 57

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 1 (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:amyA
Ordered Locus Names:DICTH_0745
OrganismiDictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
Taxonomic identifieri309799 [NCBI]
Taxonomic lineageiBacteriaDictyoglomiDictyoglomalesDictyoglomaceaeDictyoglomus
Proteomesi
  • UP000001733 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001845712 – 686Alpha-amylase 1Add BLAST685

Interactioni

Protein-protein interaction databases

STRINGi309799.DICTH_0745

Structurei

3D structure databases

SMRiP09961
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 57 family.Curated

Phylogenomic databases

eggNOGiENOG4105C6W Bacteria
COG1449 LUCA
HOGENOMiHOG000223207
KOiK22451
OMAiHFMSAGL
OrthoDBiPOG091H0IYA

Family and domain databases

Gene3Di2.70.98.10, 1 hit
InterProiView protein in InterPro
IPR015179 A-amylase/a-glucTrfase_C
IPR015178 A-amylase/a-glucTrfase_central
IPR011013 Gal_mutarotase_sf_dom
IPR014718 GH-type_carb-bd
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR028995 Glyco_hydro_57/38_cen_sf
IPR004300 Glyco_hydro_57_N
PfamiView protein in Pfam
PF09094 DUF1925, 1 hit
PF09095 DUF1926, 1 hit
PF03065 Glyco_hydro_57, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014405 DUF1925, 1 hit
SUPFAMiSSF74650 SSF74650, 1 hit
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09961-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTKSIYFSLG IHNHQPVGNF DFVIERAYEM SYKPLINFFF KHPDFPINVH
60 70 80 90 100
FSGFLLLWLE KNHPEYFEKL KIMAERGQIE FVSGGFYEPI LPIIPDKDKV
110 120 130 140 150
QQIKKLNKYI YDKFGQTPKG MWLAERVWEP HLVKYIAEAG IEYVVVDDAH
160 170 180 190 200
FFSVGLKEED LFGYYLMEEQ GYKLAVFPIS MKLRYLIPFA DPEETITYLD
210 220 230 240 250
KFASEDKSKI ALLFDDGEKF GLWPDTYRTV YEEGWLETFV SKIKENFLLV
260 270 280 290 300
TPVNLYTYMQ RVKPKGRIYL PTASYREMME WVLFPEAQKE LEELVEKLKT
310 320 330 340 350
ENLWDKFSPY VKGGFWRNFL AKYDESNHMQ KKMLYVWKKV QDSPNEEVKE
360 370 380 390 400
KAMEEVFQGQ ANDAYWHGIF GGLYLPHLRT AIYEHLIKAE NYLENSEIRF
410 420 430 440 450
NIFDFDCDGN DEIIVESPFF NLYLSPNHGG SVLEWDFKTK AFNLTNVLTR
460 470 480 490 500
RKEAYHSKLS YVTSEAQGKS IHERWTAKEE GLENILFYDN HRRVSFTEKI
510 520 530 540 550
FESEPVLEDL WKDSSRLEVD SFYENYDYEI NKDENKIRVL FSGVFRGFEL
560 570 580 590 600
CKSYILYKDK SFVDVVYEIK NVSETPISLN FGWEINLNFL APNHPDYYFL
610 620 630 640 650
IGDQKYPLSS FGIEKVNNWK IFSGIGIELE CVLDVEASLY RYPIETVSLS
660 670 680
EEGFERVYQG SALIHFYKVD LPVGSTWRTT IRFWVK
Length:686
Mass (Da):81,192
Last modified:January 23, 2007 - v2
Checksum:iDFF73F94A439E187
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07896 Genomic DNA Translation: CAA30735.1
CP001146 Genomic DNA Translation: ACI18637.1
PIRiS00628 ALDYAT
RefSeqiWP_012547269.1, NC_011297.1

Genome annotation databases

EnsemblBacteriaiACI18637; ACI18637; DICTH_0745
KEGGidth:DICTH_0745

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07896 Genomic DNA Translation: CAA30735.1
CP001146 Genomic DNA Translation: ACI18637.1
PIRiS00628 ALDYAT
RefSeqiWP_012547269.1, NC_011297.1

3D structure databases

SMRiP09961
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi309799.DICTH_0745

Protein family/group databases

CAZyiGH57 Glycoside Hydrolase Family 57

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI18637; ACI18637; DICTH_0745
KEGGidth:DICTH_0745

Phylogenomic databases

eggNOGiENOG4105C6W Bacteria
COG1449 LUCA
HOGENOMiHOG000223207
KOiK22451
OMAiHFMSAGL
OrthoDBiPOG091H0IYA

Enzyme and pathway databases

BioCyciDTHE309799:G1GCP-719-MONOMER

Family and domain databases

Gene3Di2.70.98.10, 1 hit
InterProiView protein in InterPro
IPR015179 A-amylase/a-glucTrfase_C
IPR015178 A-amylase/a-glucTrfase_central
IPR011013 Gal_mutarotase_sf_dom
IPR014718 GH-type_carb-bd
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR028995 Glyco_hydro_57/38_cen_sf
IPR004300 Glyco_hydro_57_N
PfamiView protein in Pfam
PF09094 DUF1925, 1 hit
PF09095 DUF1926, 1 hit
PF03065 Glyco_hydro_57, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014405 DUF1925, 1 hit
SUPFAMiSSF74650 SSF74650, 1 hit
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY1_DICT6
AccessioniPrimary (citable) accession number: P09961
Secondary accession number(s): B5YDK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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