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Protein

Intestinal-type alkaline phosphatase

Gene

ALPI

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

In most mammals there are four different isozymes: placental, germ cell, intestinal and tissue non-specific (liver/bone/kidney).

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61MagnesiumBy similarity1
Metal bindingi61Zinc 1By similarity1
Active sitei111Phosphoserine intermediate1
Metal bindingi111Zinc 1By similarity1
Metal bindingi174MagnesiumBy similarity1
Metal bindingi330MagnesiumBy similarity1
Metal bindingi335Zinc 2By similarity1
Metal bindingi339Zinc 2; via tele nitrogenBy similarity1
Metal bindingi376Zinc 1By similarity1
Metal bindingi377Zinc 1; via tele nitrogenBy similarity1
Metal bindingi451Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protease binding Source: ParkinsonsUK-UCL
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1483166 Synthesis of PA
R-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins
R-HSA-8935690 Digestion
SABIO-RKiP09923

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase (EC:3.1.3.1)
Short name:
IAP
Short name:
Intestinal alkaline phosphatase
Gene namesi
Name:ALPI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000163295.4
HGNCiHGNC:437 ALPI
MIMi171740 gene
neXtProtiNX_P09923

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi248
OpenTargetsiENSG00000163295
PharmGKBiPA24728

Chemistry databases

ChEMBLiCHEMBL5573

Polymorphism and mutation databases

BioMutaiALPI
DMDMi130744

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 192 PublicationsAdd BLAST19
ChainiPRO_000002403720 – 503Intestinal-type alkaline phosphataseAdd BLAST484
PropeptideiPRO_0000024038504 – 528Removed in mature formBy similarityAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi140 ↔ 202By similarity
Glycosylationi141N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi268N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi429N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi486 ↔ 493By similarity
Lipidationi503GPI-anchor amidated aspartateBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

EPDiP09923
MaxQBiP09923
PaxDbiP09923
PeptideAtlasiP09923
PRIDEiP09923
ProteomicsDBi52281

PTM databases

DEPODiP09923
GlyConnecti11
iPTMnetiP09923
PhosphoSitePlusiP09923
UniCarbKBiP09923

Expressioni

Gene expression databases

BgeeiENSG00000163295 Expressed in 28 organ(s), highest expression level in jejunal mucosa
CleanExiHS_ALPI
ExpressionAtlasiP09923 baseline and differential
GenevisibleiP09923 HS

Organism-specific databases

HPAiHPA038764
HPA038765
HPA051699

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106749, 18 interactors
IntActiP09923, 9 interactors
MINTiP09923
STRINGi9606.ENSP00000295463

Chemistry databases

BindingDBiP09923

Structurei

3D structure databases

ProteinModelPortaliP09923
SMRiP09923
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126 Eukaryota
COG1785 LUCA
GeneTreeiENSGT00390000008704
HOGENOMiHOG000099118
HOVERGENiHBG007345
InParanoidiP09923
KOiK01077
OMAiYVWSRKG
OrthoDBiEOG091G067H
PhylomeDBiP09923
TreeFamiTF323513

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P09923-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQGPWVLLLL GLRLQLSLGV IPAEEENPAF WNRQAAEALD AAKKLQPIQK
60 70 80 90 100
VAKNLILFLG DGLGVPTVTA TRILKGQKNG KLGPETPLAM DRFPYLALSK
110 120 130 140 150
TYNVDRQVPD SAATATAYLC GVKANFQTIG LSAAARFNQC NTTRGNEVIS
160 170 180 190 200
VMNRAKQAGK SVGVVTTTRV QHASPAGTYA HTVNRNWYSD ADMPASARQE
210 220 230 240 250
GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPADA SQNGIRLDGK
260 270 280 290 300
NLVQEWLAKH QGAWYVWNRT ELMQASLDQS VTHLMGLFEP GDTKYEIHRD
310 320 330 340 350
PTLDPSLMEM TEAALRLLSR NPRGFYLFVE GGRIDHGHHE GVAYQALTEA
360 370 380 390 400
VMFDDAIERA GQLTSEEDTL TLVTADHSHV FSFGGYTLRG SSIFGLAPSK
410 420 430 440 450
AQDSKAYTSI LYGNGPGYVF NSGVRPDVNE SESGSPDYQQ QAAVPLSSET
460 470 480 490 500
HGGEDVAVFA RGPQAHLVHG VQEQSFVAHV MAFAACLEPY TACDLAPPAC
510 520
TTDAAHPVAA SLPLLAGTLL LLGASAAP
Length:528
Mass (Da):56,812
Last modified:April 1, 1990 - v2
Checksum:i465306BEDF9F0B79
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WEQ0F8WEQ0_HUMAN
Intestinal-type alkaline phosphatas...
ALPI
50Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti347L → V in AAA51703 (PubMed:3469665).Curated1
Sequence conflicti410I → T in AAA51704 (PubMed:3468508).Curated1
Sequence conflicti497P → L in AAA51703 (PubMed:3469665).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050524144R → H. Corresponds to variant dbSNP:rs7559279Ensembl.1
Natural variantiVAR_011816298H → L. Corresponds to variant dbSNP:rs1047223Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15694 mRNA Translation: AAA51703.1
M31008 mRNA Translation: AAA51704.1
J03930 Genomic DNA Translation: AAA98617.1
AK313163 mRNA Translation: BAG35981.1
AC068134 Genomic DNA Translation: AAY24089.1
CH471063 Genomic DNA Translation: EAW70997.1
BC132678 mRNA Translation: AAI32679.1
Y00512 Genomic DNA Translation: CAA68564.1
M19161 Genomic DNA Translation: AAA51705.1
CCDSiCCDS2492.1
PIRiA31073 PAHUI
RefSeqiNP_001622.2, NM_001631.4
UniGeneiHs.284255
Hs.37009

Genome annotation databases

EnsembliENST00000295463; ENSP00000295463; ENSG00000163295
GeneIDi248
KEGGihsa:248
UCSCiuc002vst.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

Alkaline phosphatase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15694 mRNA Translation: AAA51703.1
M31008 mRNA Translation: AAA51704.1
J03930 Genomic DNA Translation: AAA98617.1
AK313163 mRNA Translation: BAG35981.1
AC068134 Genomic DNA Translation: AAY24089.1
CH471063 Genomic DNA Translation: EAW70997.1
BC132678 mRNA Translation: AAI32679.1
Y00512 Genomic DNA Translation: CAA68564.1
M19161 Genomic DNA Translation: AAA51705.1
CCDSiCCDS2492.1
PIRiA31073 PAHUI
RefSeqiNP_001622.2, NM_001631.4
UniGeneiHs.284255
Hs.37009

3D structure databases

ProteinModelPortaliP09923
SMRiP09923
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106749, 18 interactors
IntActiP09923, 9 interactors
MINTiP09923
STRINGi9606.ENSP00000295463

Chemistry databases

BindingDBiP09923
ChEMBLiCHEMBL5573

PTM databases

DEPODiP09923
GlyConnecti11
iPTMnetiP09923
PhosphoSitePlusiP09923
UniCarbKBiP09923

Polymorphism and mutation databases

BioMutaiALPI
DMDMi130744

Proteomic databases

EPDiP09923
MaxQBiP09923
PaxDbiP09923
PeptideAtlasiP09923
PRIDEiP09923
ProteomicsDBi52281

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295463; ENSP00000295463; ENSG00000163295
GeneIDi248
KEGGihsa:248
UCSCiuc002vst.4 human

Organism-specific databases

CTDi248
DisGeNETi248
EuPathDBiHostDB:ENSG00000163295.4
GeneCardsiALPI
HGNCiHGNC:437 ALPI
HPAiHPA038764
HPA038765
HPA051699
MIMi171740 gene
neXtProtiNX_P09923
OpenTargetsiENSG00000163295
PharmGKBiPA24728
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4126 Eukaryota
COG1785 LUCA
GeneTreeiENSGT00390000008704
HOGENOMiHOG000099118
HOVERGENiHBG007345
InParanoidiP09923
KOiK01077
OMAiYVWSRKG
OrthoDBiEOG091G067H
PhylomeDBiP09923
TreeFamiTF323513

Enzyme and pathway databases

ReactomeiR-HSA-1483166 Synthesis of PA
R-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins
R-HSA-8935690 Digestion
SABIO-RKiP09923

Miscellaneous databases

ChiTaRSiALPI human
GeneWikiiALPI
GenomeRNAii248
PROiPR:P09923
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163295 Expressed in 28 organ(s), highest expression level in jejunal mucosa
CleanExiHS_ALPI
ExpressionAtlasiP09923 baseline and differential
GenevisibleiP09923 HS

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPBI_HUMAN
AccessioniPrimary (citable) accession number: P09923
Secondary accession number(s): B2R7Y4
, Q53S80, Q9UBV5, Q9UCL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 1, 1990
Last modified: October 10, 2018
This is version 187 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. SIMILARITY comments
    Index of protein domains and families
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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