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Protein

Granulocyte colony-stimulating factor

Gene

CSF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Caution

PubMed:2420009 misquotes the gene name as "CSF1".Curated

GO - Molecular functioni

  • cytokine activity Source: BHF-UCL
  • enzyme binding Source: UniProtKB
  • granulocyte colony-stimulating factor receptor binding Source: GO_Central
  • growth factor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionCytokine, Growth factor

Enzyme and pathway databases

ReactomeiR-HSA-449836 Other interleukin signaling
R-HSA-6783783 Interleukin-10 signaling
SignaLinkiP09919
SIGNORiP09919

Names & Taxonomyi

Protein namesi
Recommended name:
Granulocyte colony-stimulating factor
Short name:
G-CSF
Alternative name(s):
Pluripoietin
INN: Filgrastim
INN: Lenograstim
Gene namesi
Name:CSF3
Synonyms:C17orf33, GCSF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000108342.12
HGNCiHGNC:2438 CSF3
MIMi138970 gene
neXtProtiNX_P09919

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).

Organism-specific databases

DisGeNETi1440
OpenTargetsiENSG00000108342
PharmGKBiPA26941

Protein family/group databases

Allergomei8367 Hom s G-CSF

Polymorphism and mutation databases

BioMutaiCSF3
DMDMi117564

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
ChainiPRO_000001557030 – 207Granulocyte colony-stimulating factorAdd BLAST178

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi69 ↔ 75
Disulfide bondi97 ↔ 107
Glycosylationi166O-linked (GalNAc...) threonineBy similarity1

Post-translational modificationi

O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP09919
PeptideAtlasiP09919
PRIDEiP09919
ProteomicsDBi52279
52280 [P09919-2]

PTM databases

iPTMnetiP09919
PhosphoSitePlusiP09919

Expressioni

Gene expression databases

BgeeiENSG00000108342
CleanExiHS_CSF3
ExpressionAtlasiP09919 baseline and differential
GenevisibleiP09919 HS

Interactioni

Subunit structurei

Monomer.2 Publications

GO - Molecular functioni

  • cytokine activity Source: BHF-UCL
  • enzyme binding Source: UniProtKB
  • granulocyte colony-stimulating factor receptor binding Source: GO_Central
  • growth factor activity Source: GO_Central

Protein-protein interaction databases

BioGridi107827, 10 interactors
DIPiDIP-61120N
IntActiP09919, 2 interactors
STRINGi9606.ENSP00000225474

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 39Combined sources3
Helixi41 – 65Combined sources25
Helixi69 – 72Combined sources4
Helixi77 – 87Combined sources11
Beta strandi99 – 101Combined sources3
Helixi104 – 124Combined sources21
Turni125 – 127Combined sources3
Turni130 – 132Combined sources3
Helixi133 – 157Combined sources25
Beta strandi166 – 168Combined sources3
Helixi176 – 201Combined sources26
Turni203 – 205Combined sources3

3D structure databases

ProteinModelPortaliP09919
SMRiP09919
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09919

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-6 superfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIM2 Eukaryota
ENOG4111DA4 LUCA
GeneTreeiENSGT00390000017328
HOGENOMiHOG000015371
HOVERGENiHBG005411
InParanoidiP09919
KOiK05423
OMAiKLCHPEE
OrthoDBiEOG091G0NDU
PhylomeDBiP09919
TreeFamiTF337698

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR030474 IL-6/GCSF/MGF
IPR030473 IL6/GCSF/MGF_CS
PfamiView protein in Pfam
PF16647 GCSF, 1 hit
PIRSFiPIRSF001935 IL6_MGF_GCSF, 1 hit
PRINTSiPR00433 IL6GCSFMGF
SMARTiView protein in SMART
SM00126 IL6, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit
PROSITEiView protein in PROSITE
PS00254 INTERLEUKIN_6, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P09919-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ
60 70 80 90 100
VRKIQGDGAA LQEKLVSECA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ
110 120 130 140 150
ALQLAGCLSQ LHSGLFLYQG LLQALEGISP ELGPTLDTLQ LDVADFATTI
160 170 180 190 200
WQQMEELGMA PALQPTQGAM PAFASAFQRR AGGVLVASHL QSFLEVSYRV

LRHLAQP
Length:207
Mass (Da):22,293
Last modified:July 1, 1989 - v1
Checksum:i421F635ECC776996
GO
Isoform Short (identifier: P09919-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     66-68: Missing.

Show »
Length:204
Mass (Da):21,978
Checksum:i5F07C52F13D6A6D3
GO
Isoform 3 (identifier: P09919-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-104: Missing.

Note: No experimental confirmation available.
Show »
Length:171
Mass (Da):18,434
Checksum:iC62B366732AD7664
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30A → M AA sequence (PubMed:8554326).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_013073157L → M1 PublicationCorresponds to variant dbSNP:rs2227329Ensembl.1
Natural variantiVAR_013074174A → T1 PublicationCorresponds to variant dbSNP:rs2227330Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00267366 – 68Missing in isoform Short. 3 Publications3
Alternative sequenceiVSP_04524669 – 104Missing in isoform 3. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03438 mRNA Translation: CAA27168.1
X03656 Genomic DNA Translation: CAA27291.1
X03655 mRNA Translation: CAA27290.1
M17706 mRNA Translation: AAA35882.1
AF388025 Genomic DNA Translation: AAK62469.1
CD013926 mRNA No translation available.
AC090844 Genomic DNA No translation available.
M13008 mRNA Translation: AAA03056.1
CCDSiCCDS11357.1 [P09919-1]
CCDS11358.2 [P09919-3]
CCDS42314.1 [P09919-2]
PIRiA24573
A25093 FQHUGL
RefSeqiNP_000750.1, NM_000759.3 [P09919-1]
NP_001171618.1, NM_001178147.1
NP_757373.1, NM_172219.2 [P09919-2]
NP_757374.2, NM_172220.2 [P09919-3]
UniGeneiHs.2233

Genome annotation databases

EnsembliENST00000225474; ENSP00000225474; ENSG00000108342 [P09919-1]
ENST00000394148; ENSP00000377704; ENSG00000108342 [P09919-3]
ENST00000394149; ENSP00000377705; ENSG00000108342 [P09919-2]
GeneIDi1440
KEGGihsa:1440
UCSCiuc002htp.4 human [P09919-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCSF3_HUMAN
AccessioniPrimary (citable) accession number: P09919
Secondary accession number(s): A8MXR7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 20, 2018
This is version 195 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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