UniProtKB - P09895 (RL5_RAT)
60S ribosomal protein L5
Rpl5
Functioni
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53. Interacts with RRP1B.
By similarityGO - Molecular functioni
- 5S rRNA binding Source: RGD
- mRNA 3'-UTR binding Source: RGD
- mRNA 5'-UTR binding Source: RGD
- mRNA binding Source: RGD
- structural constituent of ribosome Source: RGD
- ubiquitin ligase inhibitor activity Source: RGD
- ubiquitin protein ligase binding Source: RGD
GO - Biological processi
- cellular response to inorganic substance Source: RGD
- negative regulation of protein neddylation Source: RGD
- negative regulation of ubiquitin-dependent protein catabolic process Source: RGD
- negative regulation of ubiquitin protein ligase activity Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of isoleucine-tRNA ligase activity Source: RGD
- positive regulation of methionine-tRNA ligase activity Source: RGD
- positive regulation of phosphatase activity Source: RGD
- positive regulation of threonine-tRNA ligase activity Source: RGD
- positive regulation of translation Source: RGD
- protein stabilization Source: RGD
- regulation of signal transduction by p53 class mediator Source: RGD
- ribosomal large subunit assembly Source: RGD
- ribosomal large subunit biogenesis Source: RGD
- rRNA processing Source: RGD
- translation Source: InterPro
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding |
Enzyme and pathway databases
Reactomei | R-RNO-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-RNO-1799339, SRP-dependent cotranslational protein targeting to membrane R-RNO-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-RNO-72689, Formation of a pool of free 40S subunits R-RNO-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-RNO-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-RNO-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: 60S ribosomal protein L5 |
Gene namesi | Name:Rpl5 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619825, Rpl5 |
Subcellular locationi
Cytosol
- cytosolic large ribosomal subunit Source: RGD
- cytosolic ribosome Source: RGD
Nucleus
- nucleolus Source: UniProtKB
- nucleoplasm Source: RGD
- nucleus Source: RGD
Other locations
- aminoacyl-tRNA synthetase multienzyme complex Source: RGD
- cytoplasm Source: UniProtKB
- postsynaptic density Source: SynGO
- protein-containing complex Source: RGD
- ribonucleoprotein complex Source: RGD
- synapse Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000131436 | 2 – 297 | 60S ribosomal protein L5Add BLAST | 296 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylglycineBy similarity | 1 | |
Modified residuei | 5 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 48 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 185 | PhosphoserineBy similarity | 1 | |
Modified residuei | 220 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 220 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 220 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 232 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 272 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P09895 |
PaxDbi | P09895 |
PRIDEi | P09895 |
PTM databases
iPTMneti | P09895 |
PhosphoSitePlusi | P09895 |
SwissPalmi | P09895 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000023529, Expressed in spleen and 21 other tissues |
Genevisiblei | P09895, RN |
Interactioni
Subunit structurei
Component of the large ribosomal subunit (LSU).
Part of a LSU subcomplex, the 5S RNP which is composed of the 5S RNA, RPL5 and RPL11.
Interacts with NVL in an ATP-dependent manner.
By similarityBinary interactionsi
P09895
With | #Exp. | IntAct |
---|---|---|
Ppp1cb [P62142] | 2 | EBI-916235,EBI-352326 |
GO - Molecular functioni
- ubiquitin protein ligase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249635, 2 interactors |
IntActi | P09895, 11 interactors |
MINTi | P09895 |
STRINGi | 10116.ENSRNOP00000036514 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 253 – 297 | DisorderedSequence analysisAdd BLAST | 45 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 271 – 297 | Basic and acidic residuesSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0875, Eukaryota |
GeneTreei | ENSGT00950000183210 |
HOGENOMi | CLU_056222_1_0_1 |
InParanoidi | P09895 |
OMAi | KSQFQGY |
OrthoDBi | 999609at2759 |
PhylomeDBi | P09895 |
TreeFami | TF300044 |
Family and domain databases
HAMAPi | MF_01337_A, Ribosomal_L18_A, 1 hit |
InterProi | View protein in InterPro IPR005485, Rbsml_L5_euk/L18_arc IPR025607, Rbsml_L5e_C |
PANTHERi | PTHR23410, PTHR23410, 1 hit |
Pfami | View protein in Pfam PF14204, Ribosomal_L18_c, 1 hit PF17144, Ribosomal_L5e, 1 hit |
PRINTSi | PR00058, RIBOSOMALL5 |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGFVKVVKNK AYFKRYQVRF RRRREGKTDY YARKRLVIQD KNKYNTPKYR
60 70 80 90 100
MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVKV GLTNYAAAYC
110 120 130 140 150
TGLLLARRLL NRFGMDKIYE GQVEVNGDEY NVESIDGQPG AFTCYLDAGL
160 170 180 190 200
ARTTTGNKVF GALKGAVDGG LSIPHSTKRF PGYDSESKEF NAEVHRKHIM
210 220 230 240 250
GQNVADYMRY LMEEDEDAYK KQFSQYIKNN VTPDMMEEMY KKAHAAIREN
260 270 280 290
PVYEKKPKRE VKKKRWNRPK MSLAQKKDRV AQKKASFLRA QERAAES
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A8I6A0J9 | A0A8I6A0J9_RAT | 60S ribosomal protein L5 | Rpl5 | 278 | Annotation score: | ||
A0A8I6ARH8 | A0A8I6ARH8_RAT | 60S ribosomal protein L5 | Rpl5 | 349 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 236 | Missing in AAA42074 (PubMed:3624282).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17419 mRNA Translation: AAA42074.1 X06148 mRNA Translation: CAA29506.1 BC060561 mRNA Translation: AAH60561.1 |
PIRi | S00111, R5RTL5 |
RefSeqi | NP_112361.1, NM_031099.1 XP_006250647.1, XM_006250585.3 |
Genome annotation databases
Ensembli | ENSRNOT00000030428; ENSRNOP00000036514; ENSRNOG00000023529 |
GeneIDi | 81763 |
KEGGi | rno:81763 |
UCSCi | RGD:619825, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17419 mRNA Translation: AAA42074.1 X06148 mRNA Translation: CAA29506.1 BC060561 mRNA Translation: AAH60561.1 |
PIRi | S00111, R5RTL5 |
RefSeqi | NP_112361.1, NM_031099.1 XP_006250647.1, XM_006250585.3 |
3D structure databases
AlphaFoldDBi | P09895 |
SMRi | P09895 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249635, 2 interactors |
IntActi | P09895, 11 interactors |
MINTi | P09895 |
STRINGi | 10116.ENSRNOP00000036514 |
PTM databases
iPTMneti | P09895 |
PhosphoSitePlusi | P09895 |
SwissPalmi | P09895 |
Proteomic databases
jPOSTi | P09895 |
PaxDbi | P09895 |
PRIDEi | P09895 |
Genome annotation databases
Ensembli | ENSRNOT00000030428; ENSRNOP00000036514; ENSRNOG00000023529 |
GeneIDi | 81763 |
KEGGi | rno:81763 |
UCSCi | RGD:619825, rat |
Organism-specific databases
CTDi | 6125 |
RGDi | 619825, Rpl5 |
Phylogenomic databases
eggNOGi | KOG0875, Eukaryota |
GeneTreei | ENSGT00950000183210 |
HOGENOMi | CLU_056222_1_0_1 |
InParanoidi | P09895 |
OMAi | KSQFQGY |
OrthoDBi | 999609at2759 |
PhylomeDBi | P09895 |
TreeFami | TF300044 |
Enzyme and pathway databases
Reactomei | R-RNO-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-RNO-1799339, SRP-dependent cotranslational protein targeting to membrane R-RNO-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-RNO-72689, Formation of a pool of free 40S subunits R-RNO-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-RNO-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-RNO-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
PROi | PR:P09895 |
Gene expression databases
Bgeei | ENSRNOG00000023529, Expressed in spleen and 21 other tissues |
Genevisiblei | P09895, RN |
Family and domain databases
HAMAPi | MF_01337_A, Ribosomal_L18_A, 1 hit |
InterProi | View protein in InterPro IPR005485, Rbsml_L5_euk/L18_arc IPR025607, Rbsml_L5e_C |
PANTHERi | PTHR23410, PTHR23410, 1 hit |
Pfami | View protein in Pfam PF14204, Ribosomal_L18_c, 1 hit PF17144, Ribosomal_L5e, 1 hit |
PRINTSi | PR00058, RIBOSOMALL5 |
MobiDBi | Search... |
Entry informationi
Entry namei | RL5_RAT | |
Accessioni | P09895Primary (citable) accession number: P09895 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Ribosomal proteins
Ribosomal proteins families and list of entries - SIMILARITY comments
Index of protein domains and families