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Protein

Colicin-E9

Gene

col

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi550ZincBy similarity1
Metal bindingi575ZincBy similarity1
Metal bindingi579ZincBy similarity1

GO - Molecular functioni

  • endonuclease activity Source: CAFA
  • metal ion binding Source: UniProtKB-KW
  • protein domain specific binding Source: CAFA

GO - Biological processi

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Bacteriocin, Endonuclease, Hydrolase, Nuclease
LigandMetal-binding, Zinc

Protein family/group databases

TCDBi1.C.1.4.1 the channel-forming colicin (colicin) family

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E9 (EC:3.1.-.-)
Gene namesi
Name:col
Synonyms:cei
Encoded oniPlasmid ColE90 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002186841 – 582Colicin-E9Add BLAST582

Proteomic databases

PRIDEiP09883

Interactioni

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-16992N
IntActiP09883, 6 interactors
MINTiP09883

Structurei

Secondary structure

1582
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00342
ProteinModelPortaliP09883
SMRiP09883
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09883

Family & Domainsi

Sequence similaritiesi

Belongs to the colicin/pyosin nuclease family.Curated

Family and domain databases

CDDicd00085 HNHc, 1 hit
Gene3Di3.90.540.10, 1 hit
InterProiView protein in InterPro
IPR024575 Cloacin_colicin_fam
IPR037146 Colicin/pyocin_DNase_dom_sf
IPR024566 Colicin_R_dom
IPR003615 HNH_nuc
IPR016128 Pyosin/cloacin_T_dom
IPR036302 Pyosin/cloacin_T_dom_sf
PfamiView protein in Pfam
PF03515 Cloacin, 1 hit
PF11570 E2R135, 1 hit
PRINTSiPR01295 CLOACIN
SUPFAMiSSF69369 SSF69369, 1 hit

Sequencei

Sequence statusi: Complete.

P09883-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGGDGRGHN TGAHSTSGNI NGGPTGIGVS GGASDGSGWS SENNPWGGGS
60 70 80 90 100
GSGIHWGGGS GRGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA
110 120 130 140 150
GGLAVSISAS ELSAAIAGII AKLKKVNLKF TPFGVVLSSL IPSEIAKDDP
160 170 180 190 200
NMMSKIVTSL PADDITESPV SSLPLDKATV NVNVRVVDDV KDERQNISVV
210 220 230 240 250
SGVPMSVPVV DAKPTERPGV FTASIPGAPV LNISVNDSTP AVQTLSPGVT
260 270 280 290 300
NNTDKDVRPA GFTQGGNTRD AVIRFPKDSG HNAVYVSVSD VLSPDQVKQR
310 320 330 340 350
QDEENRRQQE WDATHPVEAA ERNYERARAE LNQANEDVAR NQERQAKAVQ
360 370 380 390 400
VYNSRKSELD AANKTLADAI AEIKQFNRFA HDPMAGGHRM WQMAGLKAQR
410 420 430 440 450
AQTDVNNKQA AFDAAAKEKS DADAALSAAQ ERRKQKENKE KDAKDKLDKE
460 470 480 490 500
SKRNKPGKAT GKGKPVGDKW LDDAGKDSGA PIPDRIADKL RDKEFKSFDD
510 520 530 540 550
FRKAVWEEVS KDPELSKNLN PSNKSSVSKG YSPFTPKNQQ VGGRKVYELH
560 570 580
HDKPISQGGE VYDMDNIRVT TPKRHIDIHR GK
Length:582
Mass (Da):61,587
Last modified:October 1, 1996 - v4
Checksum:i47A71B57B45AFDD9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti511K → R (PubMed:2646600).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12591 Genomic DNA Translation: CAA31104.1
X15858 Genomic DNA Translation: CAA33862.1
PIRiPQ0032
RefSeqiWP_012644886.1, NC_011977.1
YP_002533537.1, NC_011977.1

Genome annotation databases

GeneIDi7377410

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12591 Genomic DNA Translation: CAA31104.1
X15858 Genomic DNA Translation: CAA33862.1
PIRiPQ0032
RefSeqiWP_012644886.1, NC_011977.1
YP_002533537.1, NC_011977.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BXIX-ray2.05B449-582[»]
1EMVX-ray1.70B450-582[»]
1FR2X-ray1.60B450-582[»]
1FSJX-ray1.80B/C/D/E450-582[»]
1V13X-ray2.00A/B450-582[»]
1V14X-ray2.90A/B/C/D450-582[»]
1V15X-ray2.40A/B/C/D450-582[»]
2GYKX-ray1.60B/F450-582[»]
2GZEX-ray1.80B450-582[»]
2GZFX-ray1.75B450-582[»]
2GZGX-ray1.70B450-582[»]
2GZIX-ray1.70B450-582[»]
2GZJX-ray1.60B/F450-582[»]
2IVZX-ray2.00E/F/G/H32-47[»]
2K5XNMR-B450-582[»]
2VLNX-ray1.60B450-582[»]
2VLOX-ray1.80B450-582[»]
2VLPX-ray2.00B450-582[»]
2VLQX-ray1.60B450-582[»]
2WPTX-ray1.78B450-582[»]
3O0EX-ray3.01L/M/N/O/P/Q2-18[»]
4JMLX-ray2.00E32-47[»]
5EW5X-ray3.20A/B/C/D1-582[»]
DisProtiDP00342
ProteinModelPortaliP09883
SMRiP09883
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-16992N
IntActiP09883, 6 interactors
MINTiP09883

Protein family/group databases

TCDBi1.C.1.4.1 the channel-forming colicin (colicin) family

Proteomic databases

PRIDEiP09883

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi7377410

Miscellaneous databases

EvolutionaryTraceiP09883

Family and domain databases

CDDicd00085 HNHc, 1 hit
Gene3Di3.90.540.10, 1 hit
InterProiView protein in InterPro
IPR024575 Cloacin_colicin_fam
IPR037146 Colicin/pyocin_DNase_dom_sf
IPR024566 Colicin_R_dom
IPR003615 HNH_nuc
IPR016128 Pyosin/cloacin_T_dom
IPR036302 Pyosin/cloacin_T_dom_sf
PfamiView protein in Pfam
PF03515 Cloacin, 1 hit
PF11570 E2R135, 1 hit
PRINTSiPR01295 CLOACIN
SUPFAMiSSF69369 SSF69369, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCEA9_ECOLX
AccessioniPrimary (citable) accession number: P09883
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 112 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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