UniProtKB - P09838 (TDT_MOUSE)
Protein
DNA nucleotidylexotransferase
Gene
Dntt
Organism
Mus musculus (Mouse)
Status
Functioni
Transferase that catalyzes the nontemplated addition of nucleoside triphosphate to coding ends during V(D)J recombination (N addition). Involved in the generation of diversity in the antigen-binding region of immunoglobulin heavy and light chains and T-cell receptors during B- and T-cell development. Does not act on double-stranded DNA with blunt ends.5 Publications
3'-to-5' DNA exonuclease. Involved in the generation of diversity in the antigen-binding region of immunoglobulin heavy and light chains and T-cell receptors during B- and T-cell development. Acts on single-stranded and double-stranded DNA with 3' or 5' extensions, but not on double-stranded DNA with blunt ends. Attenuates not only isoform TDT-S-catalyzed N addition, but also P (palindromic) addition in coding joins (PubMed:11938351). Lacks terminal transferase activity (PubMed:11136823, PubMed:7556063).3 Publications
Catalytic activityi
- EC:2.7.7.312 Publications
Cofactori
Mg2+1 PublicationNote: Can also utilize other divalent cations, such as Mn2+ and Co2+ (in vitro).Curated1 Publication
Kineticsi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 343 | MagnesiumCombined sourcesCurated | 1 | |
Metal bindingi | 345 | MagnesiumCombined sourcesCurated | 1 | |
Metal bindingi | 434 | MagnesiumCombined sourcesCurated | 1 |
GO - Molecular functioni
- DNA binding Source: InterPro
- DNA-directed DNA polymerase activity Source: InterPro
- DNA nucleotidylexotransferase activity Source: MGI
- hydrolase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- DNA metabolic process Source: MGI
- DNA modification Source: UniProtKB-KW
- double-strand break repair via nonhomologous end joining Source: GO_Central
- response to ATP Source: MGI
Keywordsi
Molecular function | Hydrolase, Nucleotidyltransferase, Transferase |
Biological process | Terminal addition |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.7.7.31, 3474 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Dntt Synonyms:Tdt |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98659, Dntt |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear chromatin Source: MGI
- nuclear matrix Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: MGI
- euchromatin Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 29 | D → A: Almost complete loss of exonuclease activity in TDT-L; when associated with A-170 and A-473. Decreased transferase activity in TDT-S; when associated with A-170 and A-473. 1 Publication | 1 | |
Mutagenesisi | 170 | D → A: Almost complete loss of exonuclease activity in TDT-L; when associated with A-29 and A-473. Decreased transferase activity in TDT-S; when associated with A-29 and A-473. 1 Publication | 1 | |
Mutagenesisi | 342 | H → A: Nearly abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 398 | L → A: Nearly abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 399 | D → A: Nearly abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 400 | H → A: Reduces enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 403 | K → A: Nearly abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 473 | D → A: Almost complete loss of exonuclease activity in TDT-L; when associated with A-29 and A-170. Decreased transferase activity in TDT-S; when associated with A-29 and A-170. 1 Publication | 1 | |
Mutagenesisi | 473 | D → A: Nearly abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 475 | H → A: Nearly abolishes enzyme activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218792 | 1 – 510 | DNA nucleotidylexotransferaseAdd BLAST | 510 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 134 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P09838 |
MaxQBi | P09838 |
PaxDbi | P09838 |
PRIDEi | P09838 |
PTM databases
iPTMneti | P09838 |
PhosphoSitePlusi | P09838 |
Expressioni
Tissue specificityi
Isoform TDT-L: Expressed in the thymus, and, at lower levels, in the bone marrow (PubMed:8464703, PubMed:11136823, PubMed:7556063). Detected in both cycling and noncycling pro-B and pre-B cells (at protein level) (PubMed:11938351). Isoform TDT-S: Expressed in both cycling and noncycling pro-B, but not pre-B, cells (at protein level) (PubMed:11938351). Not detected in mature peripheral or germinal center B cells (PubMed:11938351).4 Publications
Gene expression databases
Bgeei | ENSMUSG00000025014, Expressed in thymus and 54 other tissues |
ExpressionAtlasi | P09838, baseline and differential |
Genevisiblei | P09838, MM |
Interactioni
Subunit structurei
Interacts with PRP19 and DNTTIP1.
Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.
Interacts with TRERF1.
By similarityProtein-protein interaction databases
BioGRIDi | 204095, 4 interactors |
STRINGi | 10090.ENSMUSP00000062078 |
Miscellaneous databases
RNActi | P09838, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P09838 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P09838 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 124 | BRCTPROSITE-ProRule annotationAdd BLAST | 98 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 151 – 510 | Mediates interaction with DNTTIP2By similarityAdd BLAST | 360 | |
Regioni | 258 – 262 | Involved in DNA binding2 Publications | 5 | |
Regioni | 333 – 338 | Deoxynucleoside triphosphate bindingCombined sourcesCurated | 6 | |
Regioni | 342 – 345 | Deoxynucleoside triphosphate bindingCombined sourcesCurated | 4 | |
Regioni | 449 – 450 | Deoxynucleoside triphosphate bindingCombined sourcesCurated | 2 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 11 – 17 | Nuclear localization signalBy similarity | 7 |
Sequence similaritiesi
Belongs to the DNA polymerase type-X family.Curated
Phylogenomic databases
eggNOGi | KOG2534, Eukaryota |
GeneTreei | ENSGT00940000158584 |
HOGENOMi | CLU_008698_0_0_1 |
InParanoidi | P09838 |
OMAi | YCDLVES |
OrthoDBi | 1212057at2759 |
PhylomeDBi | P09838 |
TreeFami | TF103012 |
Family and domain databases
CDDi | cd00141, NT_POLXc, 1 hit |
Gene3Di | 1.10.150.110, 1 hit 3.30.210.10, 1 hit 3.30.460.10, 1 hit 3.40.50.10190, 1 hit |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR002054, DNA-dir_DNA_pol_X IPR019843, DNA_pol-X_BS IPR010996, DNA_pol_b-like_N IPR028207, DNA_pol_B_palm_palm IPR018944, DNA_pol_lambd_fingers_domain IPR027421, DNA_pol_lamdba_lyase_dom_sf IPR037160, DNA_Pol_thumb_sf IPR022312, DNA_pol_X IPR043519, NT_sf IPR029398, PolB_thumb IPR027292, TdT IPR001726, TdT/Mu |
Pfami | View protein in Pfam PF00533, BRCT, 1 hit PF14792, DNA_pol_B_palm, 1 hit PF14791, DNA_pol_B_thumb, 1 hit PF10391, DNA_pol_lambd_f, 1 hit PF14716, HHH_8, 1 hit |
PIRSFi | PIRSF000817, DNA_NT, 1 hit PIRSF501175, TDT, 1 hit |
PRINTSi | PR00869, DNAPOLX PR00871, DNAPOLXTDT |
SMARTi | View protein in SMART SM00292, BRCT, 1 hit SM00483, POLXc, 1 hit |
SUPFAMi | SSF47802, SSF47802, 1 hit SSF52113, SSF52113, 1 hit SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit PS00522, DNA_POLYMERASE_X, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform TDT-S (identifier: P09838-2) [UniParc]FASTAAdd to basket
Also known as: TDT-Small, TdtS1 Publication
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDPLQAVHLG PRKKRPRQLG TPVASTPYDI RFRDLVLFIL EKKMGTTRRA
60 70 80 90 100
FLMELARRKG FRVENELSDS VTHIVAENNS GSDVLEWLQL QNIKASSELE
110 120 130 140 150
LLDISWLIEC MGAGKPVEMM GRHQLVVNRN SSPSPVPGSQ NVPAPAVKKI
160 170 180 190 200
SQYACQRRTT LNNYNQLFTD ALDILAENDE LRENEGSCLA FMRASSVLKS
210 220 230 240 250
LPFPITSMKD TEGIPCLGDK VKSIIEGIIE DGESSEAKAV LNDERYKSFK
260 270 280 290 300
LFTSVFGVGL KTAEKWFRMG FRTLSKIQSD KSLRFTQMQK AGFLYYEDLV
310 320 330 340 350
SCVNRPEAEA VSMLVKEAVV TFLPDALVTM TGGFRRGKMT GHDVDFLITS
360 370 380 390 400
PEATEDEEQQ LLHKVTDFWK QQGLLLYCDI LESTFEKFKQ PSRKVDALDH
410 420 430 440 450
FQKCFLILKL DHGRVHSEKS GQQEGKGWKA IRVDLVMCPY DRRAFALLGW
460 470 480 490 500
TGSRQFERDL RRYATHERKM MLDNHALYDR TKRVFLEAES EEEIFAHLGL
510
DYIEPWERNA
Isoform TDT-L (identifier: P09838-1) [UniParc]FASTAAdd to basket
Also known as: TDT-Large, TdtL1 Publication
The sequence of this isoform differs from the canonical sequence as follows:
482-482: K → KGKTVTISPLDGKVSKLQKAL
Note: Exhibits 3'-to-5' DNA exonuclease activity (EC=3.1.11.-) (PubMed:23856622). May have a shorter half-life than isoform TDT-S (PubMed:7556063, PubMed:10878023).3 Publications
Show »Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 26 | T → M in CAA48634 (PubMed:8464703).Curated | 1 | |
Sequence conflicti | 99 | L → F in CAA48634 (PubMed:8464703).Curated | 1 | |
Sequence conflicti | 193 | R → G in CAA27735 (PubMed:3755527).Curated | 1 | |
Sequence conflicti | 287 | Q → K in CAA27735 (PubMed:3755527).Curated | 1 | |
Sequence conflicti | 309 | E → Q in CAA27735 (PubMed:3755527).Curated | 1 | |
Sequence conflicti | 367 | D → H in CAA27735 (PubMed:3755527).Curated | 1 | |
Sequence conflicti | 441 – 445 | DRRAF → ECAC in CAA27735 (PubMed:3755527).Curated | 5 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_059967 | 482 | K → KGKTVTISPLDGKVSKLQKA L in isoform TDT-L. 3 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04123 mRNA Translation: CAA27735.1 X68670 mRNA Translation: CAA48634.2 AF316014 mRNA Translation: AAK07884.1 AF316015 mRNA Translation: AAK07885.1 AK087978 mRNA Translation: BAC40071.1 AK088709 mRNA Translation: BAC40518.1 |
CCDSi | CCDS29807.1 [P09838-1] CCDS37984.1 [P09838-2] |
PIRi | B23595 |
RefSeqi | NP_001036693.1, NM_001043228.1 [P09838-2] NP_033371.2, NM_009345.2 [P09838-1] |
Genome annotation databases
Ensembli | ENSMUST00000051806; ENSMUSP00000062078; ENSMUSG00000025014 [P09838-1] ENSMUST00000112200; ENSMUSP00000107819; ENSMUSG00000025014 [P09838-2] |
GeneIDi | 21673 |
KEGGi | mmu:21673 |
UCSCi | uc008hlo.1, mouse [P09838-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04123 mRNA Translation: CAA27735.1 X68670 mRNA Translation: CAA48634.2 AF316014 mRNA Translation: AAK07884.1 AF316015 mRNA Translation: AAK07885.1 AK087978 mRNA Translation: BAC40071.1 AK088709 mRNA Translation: BAC40518.1 |
CCDSi | CCDS29807.1 [P09838-1] CCDS37984.1 [P09838-2] |
PIRi | B23595 |
RefSeqi | NP_001036693.1, NM_001043228.1 [P09838-2] NP_033371.2, NM_009345.2 [P09838-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JMS | X-ray | 2.36 | A | 130-510 | [»] | |
1KDH | X-ray | 3.00 | A | 148-510 | [»] | |
1KEJ | X-ray | 3.00 | A | 148-510 | [»] | |
4I27 | X-ray | 2.60 | A | 132-510 | [»] | |
4I28 | X-ray | 2.15 | A | 132-510 | [»] | |
4I29 | X-ray | 2.20 | A | 132-510 | [»] | |
4I2A | X-ray | 1.90 | A | 132-510 | [»] | |
4I2B | X-ray | 2.20 | A | 132-510 | [»] | |
4I2C | X-ray | 2.10 | A | 132-510 | [»] | |
4I2D | X-ray | 2.30 | A | 132-510 | [»] | |
4I2E | X-ray | 2.00 | A | 132-510 | [»] | |
4I2F | X-ray | 2.10 | A | 132-510 | [»] | |
4I2G | X-ray | 2.50 | A | 132-510 | [»] | |
4I2H | X-ray | 2.75 | A | 132-510 | [»] | |
4I2I | X-ray | 2.50 | A | 132-510 | [»] | |
4I2J | X-ray | 2.70 | A | 132-510 | [»] | |
4IQT | X-ray | 2.60 | A | 132-510 | [»] | |
4IQU | X-ray | 2.40 | A | 132-510 | [»] | |
4IQV | X-ray | 2.90 | A | 132-510 | [»] | |
4IQW | X-ray | 2.60 | A | 132-510 | [»] | |
4QZ8 | X-ray | 2.70 | A | 132-510 | [»] | |
4QZ9 | X-ray | 2.05 | A | 132-510 | [»] | |
4QZA | X-ray | 2.15 | A | 132-510 | [»] | |
4QZB | X-ray | 2.15 | A | 132-510 | [»] | |
4QZC | X-ray | 2.75 | A | 132-510 | [»] | |
4QZD | X-ray | 2.70 | A | 132-510 | [»] | |
4QZE | X-ray | 2.25 | A | 132-510 | [»] | |
4QZF | X-ray | 2.60 | A | 132-510 | [»] | |
4QZG | X-ray | 2.75 | A | 132-510 | [»] | |
4QZH | X-ray | 2.60 | A | 132-510 | [»] | |
4QZI | X-ray | 2.65 | A | 132-510 | [»] | |
5D46 | X-ray | 2.80 | A | 132-510 | [»] | |
5D49 | X-ray | 1.99 | A | 132-510 | [»] | |
5D4B | X-ray | 2.66 | A/B | 132-510 | [»] | |
6GO3 | X-ray | 2.20 | A | 132-377 | [»] | |
A | 409-510 | [»] | ||||
6GO4 | X-ray | 1.96 | A | 132-377 | [»] | |
A | 409-510 | [»] | ||||
6GO5 | X-ray | 2.35 | A/B | 132-377 | [»] | |
A/B | 409-510 | [»] | ||||
6GO6 | X-ray | 2.09 | A | 132-377 | [»] | |
A | 409-510 | [»] | ||||
6GO7 | X-ray | 2.55 | A | 132-377 | [»] | |
A | 409-510 | [»] | ||||
SMRi | P09838 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204095, 4 interactors |
STRINGi | 10090.ENSMUSP00000062078 |
PTM databases
iPTMneti | P09838 |
PhosphoSitePlusi | P09838 |
Proteomic databases
jPOSTi | P09838 |
MaxQBi | P09838 |
PaxDbi | P09838 |
PRIDEi | P09838 |
Protocols and materials databases
Antibodypediai | 16924, 563 antibodies |
DNASUi | 21673 |
Genome annotation databases
Ensembli | ENSMUST00000051806; ENSMUSP00000062078; ENSMUSG00000025014 [P09838-1] ENSMUST00000112200; ENSMUSP00000107819; ENSMUSG00000025014 [P09838-2] |
GeneIDi | 21673 |
KEGGi | mmu:21673 |
UCSCi | uc008hlo.1, mouse [P09838-2] |
Organism-specific databases
CTDi | 1791 |
MGIi | MGI:98659, Dntt |
Phylogenomic databases
eggNOGi | KOG2534, Eukaryota |
GeneTreei | ENSGT00940000158584 |
HOGENOMi | CLU_008698_0_0_1 |
InParanoidi | P09838 |
OMAi | YCDLVES |
OrthoDBi | 1212057at2759 |
PhylomeDBi | P09838 |
TreeFami | TF103012 |
Enzyme and pathway databases
BRENDAi | 2.7.7.31, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 21673, 7 hits in 19 CRISPR screens |
ChiTaRSi | Dntt, mouse |
EvolutionaryTracei | P09838 |
PROi | PR:P09838 |
RNActi | P09838, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025014, Expressed in thymus and 54 other tissues |
ExpressionAtlasi | P09838, baseline and differential |
Genevisiblei | P09838, MM |
Family and domain databases
CDDi | cd00141, NT_POLXc, 1 hit |
Gene3Di | 1.10.150.110, 1 hit 3.30.210.10, 1 hit 3.30.460.10, 1 hit 3.40.50.10190, 1 hit |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR002054, DNA-dir_DNA_pol_X IPR019843, DNA_pol-X_BS IPR010996, DNA_pol_b-like_N IPR028207, DNA_pol_B_palm_palm IPR018944, DNA_pol_lambd_fingers_domain IPR027421, DNA_pol_lamdba_lyase_dom_sf IPR037160, DNA_Pol_thumb_sf IPR022312, DNA_pol_X IPR043519, NT_sf IPR029398, PolB_thumb IPR027292, TdT IPR001726, TdT/Mu |
Pfami | View protein in Pfam PF00533, BRCT, 1 hit PF14792, DNA_pol_B_palm, 1 hit PF14791, DNA_pol_B_thumb, 1 hit PF10391, DNA_pol_lambd_f, 1 hit PF14716, HHH_8, 1 hit |
PIRSFi | PIRSF000817, DNA_NT, 1 hit PIRSF501175, TDT, 1 hit |
PRINTSi | PR00869, DNAPOLX PR00871, DNAPOLXTDT |
SMARTi | View protein in SMART SM00292, BRCT, 1 hit SM00483, POLXc, 1 hit |
SUPFAMi | SSF47802, SSF47802, 1 hit SSF52113, SSF52113, 1 hit SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit PS00522, DNA_POLYMERASE_X, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TDT_MOUSE | |
Accessioni | P09838Primary (citable) accession number: P09838 Secondary accession number(s): Q99PD0, Q99PD1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | December 5, 2018 | |
Last modified: | December 2, 2020 | |
This is version 196 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families