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Protein

Glycogen phosphorylase, muscle form

Gene

Pygm

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76AMPBy similarity1
Sitei109Involved in the association of subunitsBy similarity1
Sitei143Involved in the association of subunitsBy similarity1
Sitei156May be involved in allosteric controlBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Glycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen metabolism
LigandNucleotide-binding, Pyridoxal phosphate

Protein family/group databases

CAZyiGT35 Glycosyltransferase Family 35

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen phosphorylase, muscle form (EC:2.4.1.1)
Alternative name(s):
Myophosphorylase
Gene namesi
Name:Pygm
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3461 Pygm

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001885332 – 842Glycogen phosphorylase, muscle formAdd BLAST841

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei15Phosphoserine; by PHK; in form phosphorylase ABy similarity1
Modified residuei26PhosphoserineCombined sources1
Modified residuei204PhosphotyrosineCombined sources1
Modified residuei227PhosphotyrosineCombined sources1
Modified residuei430PhosphoserineBy similarity1
Modified residuei473PhosphotyrosineCombined sources1
Modified residuei514PhosphoserineCombined sources1
Modified residuei681N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei747PhosphoserineCombined sources1
Modified residuei748PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP09812
PRIDEiP09812

PTM databases

iPTMnetiP09812
PhosphoSitePlusiP09812

Interactioni

Subunit structurei

Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A.

GO - Molecular functioni

Protein-protein interaction databases

IntActiP09812, 1 interactor
STRINGi10116.ENSRNOP00000028636

Structurei

3D structure databases

ProteinModelPortaliP09812
SMRiP09812
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Phylogenomic databases

eggNOGiKOG2099 Eukaryota
COG0058 LUCA
HOGENOMiHOG000278444
HOVERGENiHBG006848
InParanoidiP09812
PhylomeDBiP09812

Family and domain databases

CDDicd04300 GT1_Glycogen_Phosphorylase, 1 hit
InterProiView protein in InterPro
IPR011833 Glycg_phsphrylas
IPR000811 Glyco_trans_35
IPR035090 Pyridoxal_P_attach_site
PANTHERiPTHR11468 PTHR11468, 1 hit
PfamiView protein in Pfam
PF00343 Phosphorylase, 1 hit
PIRSFiPIRSF000460 Pprylas_GlgP, 1 hit
TIGRFAMsiTIGR02093 P_ylase, 1 hit
PROSITEiView protein in PROSITE
PS00102 PHOSPHORYLASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P09812-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRPLSDQDK RKQISVRGLA GVENVSDLKK NFNRHLHFTL VKDRNVATPR
60 70 80 90 100
DYYFALAHTV RDHLVDRWIR TQQHYYAKDP KRIYYLSLEL YMGRTLQNTM
110 120 130 140 150
VNLALENACD EATYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT
160 170 180 190 200
LGLAAYGYGI RYEFGIFNQK ICGGWQMEEA DDWLRYGNPW EKARPEFTLP
210 220 230 240 250
VHFYGRVEHT SQGAKWVDTQ VVLAMPYDTP VPGYRNNVVN TMRLWSAKAP
260 270 280 290 300
PYFNLKDFNV GGYIQAVLDR NLAENISRVL YPNDKFFEGK ELRLKQEYFV
310 320 330 340 350
VAATLQDIIR RFKSSKFGCR DPVRTNFDAF PDKVAIQLND THPSLAIPEL
360 370 380 390 400
IRILVDLERL DWDKAWDVTV KTCAYTNHTV LPEALERWPV HLMETLLPRH
410 420 430 440 450
LQIIYEINQR FLNRVAAAFP GDVDRLRRMS LVEEGAVKRI NMAHLCIAGS
460 470 480 490 500
HAVNGVARIH SEILKKTIFK DFYELEPHKF QNKTNGITPR RWLVLCNPGL
510 520 530 540 550
AEVIAERIGE EYISDLDQLR KLLSYLDDQA FIRDVAKVKQ ENKLKFSAYL
560 570 580 590 600
ETEYKVHINP NSLFDVQVKR IHEYKRQLLN CLHIITLYNR IKREPNRFMV
610 620 630 640 650
PRTIMIGGKA APGYHMAKMI IKLITAIGDV VNHDPAVGDR FRVIFLENYR
660 670 680 690 700
VSLAEKVIPA ADLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
710 720 730 740 750
AEEAGEDNFF IFGMRVEDVE RLDQRGYNAQ EYYDRIPELR QIIEQLSSGF
760 770 780 790 800
FSPKQPDLFK DIVNMVMHHD RFKVFADYEE YIKCQDKVSE LYKNPREWTR
810 820 830 840
MVIRNIATSG KFSSDRTIAQ YAREIWGLEP SRQRLPAPDE KI
Length:842
Mass (Da):97,273
Last modified:January 23, 2007 - v5
Checksum:iEA30BBB63FE69700
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V8V3G3V8V3_RAT
Alpha-1,4 glucan phosphorylase
Pygm rCG_48197
842Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti640 – 641RF → L (PubMed:2424788).Curated2
Sequence conflicti724Q → N (PubMed:2424788).Curated1
Sequence conflicti766V → L in CAA26835 (PubMed:3840433).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10669 mRNA Translation: AAA41253.1
X03032 mRNA Translation: CAA26835.1
PIRiS34624
UniGeneiRn.11238

Genome annotation databases

UCSCiRGD:3461 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10669 mRNA Translation: AAA41253.1
X03032 mRNA Translation: CAA26835.1
PIRiS34624
UniGeneiRn.11238

3D structure databases

ProteinModelPortaliP09812
SMRiP09812
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09812, 1 interactor
STRINGi10116.ENSRNOP00000028636

Protein family/group databases

CAZyiGT35 Glycosyltransferase Family 35

PTM databases

iPTMnetiP09812
PhosphoSitePlusiP09812

Proteomic databases

PaxDbiP09812
PRIDEiP09812

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:3461 rat

Organism-specific databases

RGDi3461 Pygm

Phylogenomic databases

eggNOGiKOG2099 Eukaryota
COG0058 LUCA
HOGENOMiHOG000278444
HOVERGENiHBG006848
InParanoidiP09812
PhylomeDBiP09812

Miscellaneous databases

PROiPR:P09812

Family and domain databases

CDDicd04300 GT1_Glycogen_Phosphorylase, 1 hit
InterProiView protein in InterPro
IPR011833 Glycg_phsphrylas
IPR000811 Glyco_trans_35
IPR035090 Pyridoxal_P_attach_site
PANTHERiPTHR11468 PTHR11468, 1 hit
PfamiView protein in Pfam
PF00343 Phosphorylase, 1 hit
PIRSFiPIRSF000460 Pprylas_GlgP, 1 hit
TIGRFAMsiTIGR02093 P_ylase, 1 hit
PROSITEiView protein in PROSITE
PS00102 PHOSPHORYLASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPYGM_RAT
AccessioniPrimary (citable) accession number: P09812
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 140 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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