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UniProtKB - P09811 (PYGL_RAT)
Protein
Glycogen phosphorylase, liver form
Gene
Pygl
Organism
Rattus norvegicus (Rat)
Status
Functioni
Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.
By similarityCatalytic activityi
- [(1→4)-α-D-glucosyl](n) + phosphate = [(1→4)-α-D-glucosyl](n-1) + α-D-glucose 1-phosphateBy similarityEC:2.4.1.1By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
pyridoxal 5'-phosphateBy similarity
Activity regulationi
Allosterically regulated through the non-covalent binding of metabolites, being activated by AMP and inhibited by ATP, ADP, and glucose-6-phosphate. The activity is also controlled by post-translational modifications including phosphorylation and acetylation.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 76 | AMPBy similarity | 1 | |
Sitei | 109 | Involved in the association of subunitsBy similarity | 1 | |
Sitei | 143 | Involved in the association of subunitsBy similarity | 1 | |
Sitei | 156 | May be involved in allosteric controlBy similarity | 1 | |
Binding sitei | 310 | AMPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 43 – 45 | AMPBy similarity | 3 |
GO - Molecular functioni
- AMP binding Source: RGD
- ATP binding Source: RGD
- bile acid binding Source: RGD
- carbohydrate binding Source: RGD
- glucose binding Source: RGD
- glycogen phosphorylase activity Source: RGD
- identical protein binding Source: RGD
- linear malto-oligosaccharide phosphorylase activity Source: UniProtKB-EC
- purine nucleobase binding Source: RGD
- pyridoxal phosphate binding Source: GO_Central
- SHG alpha-glucan phosphorylase activity Source: UniProtKB-EC
- vitamin binding Source: RGD
GO - Biological processi
- 5-phosphoribose 1-diphosphate biosynthetic process Source: RGD
- glucose homeostasis Source: RGD
- glycogen catabolic process Source: RGD
- glycogen metabolic process Source: RGD
- necroptotic process Source: RGD
- response to bacterium Source: RGD
Keywordsi
Molecular function | Allosteric enzyme, Glycosyltransferase, Transferase |
Biological process | Carbohydrate metabolism, Glycogen metabolism |
Ligand | Nucleotide-binding, Pyridoxal phosphate |
Enzyme and pathway databases
BRENDAi | 2.4.1.1, 5301 |
Reactomei | R-RNO-6798695, Neutrophil degranulation R-RNO-70221, Glycogen breakdown (glycogenolysis) |
Protein family/group databases
CAZyi | GT35, Glycosyltransferase Family 35 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620687, Pygl |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol By similarity
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000188526 | 2 – 850 | Glycogen phosphorylase, liver formAdd BLAST | 849 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 15 | Phosphoserine; by PHK; in form phosphorylase aBy similarity | 1 | |
Modified residuei | 364 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 470 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 524 | PhosphoserineBy similarity | 1 | |
Modified residuei | 561 | PhosphoserineCombined sources | 1 | |
Modified residuei | 639 | PhosphoserineCombined sources | 1 | |
Modified residuei | 681 | N6-(pyridoxal phosphate)lysineBy similarity | 1 | |
Modified residuei | 796 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Acetylation, which is up-regulated by glucose and insulin and down-regulated by glucagon, inhibits the glycogen phosphorylase activity by promoting PPP1R3B-mediated recruitment of phosphatase PP1 and Ser-15 dephosphorylation.By similarity
Phosphorylation at Ser-15 converts inactive phosphorylase b into active phosphorylase a. Dephosphorylation of Ser-15 by phosphatase PP1 inactivates the enzyme.By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P09811 |
PaxDbi | P09811 |
PRIDEi | P09811 |
PTM databases
iPTMneti | P09811 |
PhosphoSitePlusi | P09811 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000006388, Expressed in liver and 21 other tissues |
Genevisiblei | P09811, RN |
Interactioni
Subunit structurei
GO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248952, 1 interactor |
IntActi | P09811, 2 interactors |
MINTi | P09811 |
STRINGi | 10116.ENSRNOP00000009183 |
Chemistry databases
BindingDBi | P09811 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycogen phosphorylase family.Curated
Phylogenomic databases
eggNOGi | KOG2099, Eukaryota |
GeneTreei | ENSGT00950000183148 |
HOGENOMi | CLU_010198_1_0_1 |
InParanoidi | P09811 |
OMAi | KHKRTFT |
OrthoDBi | 240595at2759 |
PhylomeDBi | P09811 |
TreeFami | TF300309 |
Family and domain databases
InterProi | View protein in InterPro IPR011833, Glycg_phsphrylas IPR000811, Glyco_trans_35 IPR035090, Pyridoxal_P_attach_site |
PANTHERi | PTHR11468, PTHR11468, 1 hit |
Pfami | View protein in Pfam PF00343, Phosphorylase, 1 hit |
PIRSFi | PIRSF000460, Pprylas_GlgP, 1 hit |
TIGRFAMsi | TIGR02093, P_ylase, 1 hit |
PROSITEi | View protein in PROSITE PS00102, PHOSPHORYLASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P09811-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKPLTDQEK RRQISIRGIV GVENVAELKK GFNRHLHFTL VKDRNVATPR
60 70 80 90 100
DYYFALAHTV RDHLVGRWIR TQQHYYDKCP KRVYYLSLEF YMGRTLQNTM
110 120 130 140 150
INLGLQNACD EAIYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT
160 170 180 190 200
LGLAAYGYGI RYEYGIFNQK IREGWQVEEA DDWLRHGNPW EKARPEFMLP
210 220 230 240 250
VHFYGRVEHT QAGTKWVDTQ VVLALPYDTP VPGYMNNTVN TMRLWSARAP
260 270 280 290 300
NDFNLQDFNV GDYIQAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
310 320 330 340 350
VAATLQDVIR RFKASKFGSK DGVGTVFDAF PDQVAIQLND THPALAIPEL
360 370 380 390 400
MRIFVDIEKL PWSKAWEITK KTFAYTNHTV LPEALERWPV DLVEKLLPRH
410 420 430 440 450
LQIIYEINQK HLDRIVALFP KDIDRMRRMS LIEEEGGKRI NMAHLCIVGC
460 470 480 490 500
HAVNGVAKIH SDIVKTQVFK DFSELEPDKF QNKTNGITPR RWLLLCNPGL
510 520 530 540 550
ADLIAEKIGE DYVKDLSQLT KLHSFVGDDI FLREIAKVKQ ENKLKFSQFL
560 570 580 590 600
EKEYKVKINP SSMFDVHVKR IHEYKRQLLN CLHVITMYNR IKKDPKKFFV
610 620 630 640 650
PRTVIIGGKA APGYHMAKMI IKLVTSVAEV VNNDPMVGSK LKVIFLENYR
660 670 680 690 700
VSLAEKVIPA TDLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
710 720 730 740 750
AEEAGEENLF IFGMRVDDVA ALDKKGYEAK EYYEALPELK LVIDQIDNGF
760 770 780 790 800
FSPNQPDLFK DIINMLFYHD RFKVFADYEA YVKCQEKVSQ LYMNQKAWNT
810 820 830 840 850
MVLRNIAASG KFSSDRTIRE YAKDIWNMEP SDLKISLSKE SSNGVNANGK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 667 | I → V in CAA27704 (PubMed:2424788).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X63515 mRNA Translation: CAA45083.1 BC070901 mRNA Translation: AAH70901.1 M85280 Genomic DNA Translation: AAA41254.1 J03080 mRNA Translation: AAA41986.1 X04069 mRNA Translation: CAA27704.1 M59460 mRNA Translation: AAA41987.1 |
PIRi | S22338 |
RefSeqi | NP_071604.1, NM_022268.1 |
Genome annotation databases
Ensembli | ENSRNOT00000009183; ENSRNOP00000009183; ENSRNOG00000006388 |
GeneIDi | 64035 |
KEGGi | rno:64035 |
UCSCi | RGD:620687, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X63515 mRNA Translation: CAA45083.1 BC070901 mRNA Translation: AAH70901.1 M85280 Genomic DNA Translation: AAA41254.1 J03080 mRNA Translation: AAA41986.1 X04069 mRNA Translation: CAA27704.1 M59460 mRNA Translation: AAA41987.1 |
PIRi | S22338 |
RefSeqi | NP_071604.1, NM_022268.1 |
3D structure databases
SMRi | P09811 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248952, 1 interactor |
IntActi | P09811, 2 interactors |
MINTi | P09811 |
STRINGi | 10116.ENSRNOP00000009183 |
Chemistry databases
BindingDBi | P09811 |
ChEMBLi | CHEMBL3239 |
Protein family/group databases
CAZyi | GT35, Glycosyltransferase Family 35 |
PTM databases
iPTMneti | P09811 |
PhosphoSitePlusi | P09811 |
Proteomic databases
jPOSTi | P09811 |
PaxDbi | P09811 |
PRIDEi | P09811 |
Genome annotation databases
Ensembli | ENSRNOT00000009183; ENSRNOP00000009183; ENSRNOG00000006388 |
GeneIDi | 64035 |
KEGGi | rno:64035 |
UCSCi | RGD:620687, rat |
Organism-specific databases
CTDi | 5836 |
RGDi | 620687, Pygl |
Phylogenomic databases
eggNOGi | KOG2099, Eukaryota |
GeneTreei | ENSGT00950000183148 |
HOGENOMi | CLU_010198_1_0_1 |
InParanoidi | P09811 |
OMAi | KHKRTFT |
OrthoDBi | 240595at2759 |
PhylomeDBi | P09811 |
TreeFami | TF300309 |
Enzyme and pathway databases
BRENDAi | 2.4.1.1, 5301 |
Reactomei | R-RNO-6798695, Neutrophil degranulation R-RNO-70221, Glycogen breakdown (glycogenolysis) |
Miscellaneous databases
PROi | PR:P09811 |
Gene expression databases
Bgeei | ENSRNOG00000006388, Expressed in liver and 21 other tissues |
Genevisiblei | P09811, RN |
Family and domain databases
InterProi | View protein in InterPro IPR011833, Glycg_phsphrylas IPR000811, Glyco_trans_35 IPR035090, Pyridoxal_P_attach_site |
PANTHERi | PTHR11468, PTHR11468, 1 hit |
Pfami | View protein in Pfam PF00343, Phosphorylase, 1 hit |
PIRSFi | PIRSF000460, Pprylas_GlgP, 1 hit |
TIGRFAMsi | TIGR02093, P_ylase, 1 hit |
PROSITEi | View protein in PROSITE PS00102, PHOSPHORYLASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PYGL_RAT | |
Accessioni | P09811Primary (citable) accession number: P09811 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 187 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families