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Protein

Tryptophan 5-hydroxylase 1

Gene

Tph1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.

Cofactori

Pathwayi: serotonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes serotonin from L-tryptophan.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tryptophan 5-hydroxylase 2 (Tph2), Tryptophan 5-hydroxylase 1 (Tph1)
  2. no protein annotated in this organism
This subpathway is part of the pathway serotonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes serotonin from L-tryptophan, the pathway serotonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei235TryptophanBy similarity1
Binding sitei257TryptophanBy similarity1
Binding sitei265TryptophanBy similarity1
Metal bindingi272IronBy similarity1
Metal bindingi277IronBy similarity1
Metal bindingi317IronBy similarity1
Binding sitei336TryptophanBy similarity1
Binding sitei366Tryptophan; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • iron ion binding Source: InterPro
  • tryptophan 5-monooxygenase activity Source: RGD

GO - Biological processi

  • bone remodeling Source: Ensembl
  • circadian rhythm Source: RGD
  • mammary gland alveolus development Source: Ensembl
  • negative regulation of ossification Source: RGD
  • positive regulation of fat cell differentiation Source: Ensembl
  • response to immobilization stress Source: RGD
  • serotonin biosynthetic process from tryptophan Source: RGD

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processSerotonin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-209931 Serotonin and melatonin biosynthesis
UniPathwayi
UPA00846;UER00799

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan 5-hydroxylase 1 (EC:1.14.16.4)
Alternative name(s):
Tryptophan 5-monooxygenase 1
Gene namesi
Name:Tph1
Synonyms:Tph
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3895 Tph1

Subcellular locationi

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4809

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002055711 – 444Tryptophan 5-hydroxylase 1Add BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58Phosphoserine; by PKASequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP09810
PRIDEiP09810

PTM databases

iPTMnetiP09810
PhosphoSitePlusiP09810

Expressioni

Gene expression databases

BgeeiENSRNOG00000011672 Expressed in 9 organ(s), highest expression level in colon

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052959

Chemistry databases

BindingDBiP09810

Structurei

3D structure databases

ProteinModelPortaliP09810
SMRiP09810
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 94ACTPROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3820 Eukaryota
COG3186 LUCA
GeneTreeiENSGT00390000010268
HOGENOMiHOG000233373
HOVERGENiHBG006841
InParanoidiP09810
KOiK00502
OMAiMENWPEL
OrthoDBiEOG091G05MZ
PhylomeDBiP09810
TreeFamiTF313327

Family and domain databases

Gene3Di1.10.800.10, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR001273 ArAA_hydroxylase
IPR018301 ArAA_hydroxylase_Fe/CU_BS
IPR036951 ArAA_hydroxylase_sf
IPR036329 Aro-AA_hydroxylase_C_sf
IPR019774 Aromatic-AA_hydroxylase_C
IPR005963 Trp_5_mOase
IPR019773 Tyrosine_3-monooxygenase-like
PANTHERiPTHR11473 PTHR11473, 1 hit
PfamiView protein in Pfam
PF00351 Biopterin_H, 1 hit
PIRSFiPIRSF000336 TH, 1 hit
PRINTSiPR00372 FYWHYDRXLASE
SUPFAMiSSF56534 SSF56534, 1 hit
TIGRFAMsiTIGR01270 Trp_5_monoox, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit
PS00367 BH4_AAA_HYDROXYL_1, 1 hit
PS51410 BH4_AAA_HYDROXYL_2, 1 hit

Sequencei

Sequence statusi: Complete.

P09810-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIEDNKENKD HSSERGRVTL IFSLKNEVGG LIKALKIFQE NHVNLLHIES
60 70 80 90 100
RKSKRRNSEF EIFVDCDINR EQLNDIFPLL KSHTTVLSVD SPDQLPEKED
110 120 130 140 150
VMETVPWFPK KISDLDFCAN RVLLYGSELD ADHPGFKDNV YRRRRKYFAE
160 170 180 190 200
LAMNYKHGDP IPKIEFTEEE IKTWGTIFRE LNKLYPTHAC REYLRNLPLL
210 220 230 240 250
SKYCGYREDN VPQLEDVSNF LKERTGFSIR PVAGYLSPRD FLSGLAFRVF
260 270 280 290 300
HCTQYVRHSS DPLYTPEPDT CHELLGHVPL LAEPSFAQFS QEIGLASLGA
310 320 330 340 350
SEETVQKLAT CYFFTVEFGL CKQDGQLRVF GAGLLSSISE LRHALSGHAK
360 370 380 390 400
VKPFDPKVAC KQECLITSFQ DVYFVSESFE DAKEKMREFA KTVKRPFGVK
410 420 430 440
YNPYTQSIQV LRDSKSITSA MNELRHDLDV VNDALARVSR WPSV
Length:444
Mass (Da):51,068
Last modified:October 1, 1989 - v2
Checksum:iC3CF5245727CC825
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28000 mRNA Translation: AAA42262.1
X53501 mRNA Translation: CAA37579.1
PIRiJL0034 WHRTW
RefSeqiNP_001094104.1, NM_001100634.2
XP_006229281.1, XM_006229219.2
UniGeneiRn.94788

Genome annotation databases

EnsembliENSRNOT00000056109; ENSRNOP00000052959; ENSRNOG00000011672
GeneIDi24848
KEGGirno:24848
UCSCiRGD:3895 rat

Similar proteinsi

Entry informationi

Entry nameiTPH1_RAT
AccessioniPrimary (citable) accession number: P09810
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1989
Last modified: September 12, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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