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Protein

Cytotoxic T-lymphocyte protein 4

Gene

Ctla4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.By similarity

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Cytotoxic T-lymphocyte protein 4
Alternative name(s):
Cytotoxic T-lymphocyte-associated antigen 4
Short name:
CTLA-4
CD_antigen: CD152
Gene namesi
Name:Ctla4
Synonyms:Cd152
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88556 Ctla4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini36 – 161ExtracellularSequence analysisAdd BLAST126
Transmembranei162 – 182HelicalSequence analysisAdd BLAST21
Topological domaini183 – 223CytoplasmicSequence analysisAdd BLAST41

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000001473536 – 223Cytotoxic T-lymphocyte protein 4Add BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi58 ↔ 129Combined sources1 Publication
Disulfide bondi85 ↔ 103Combined sources1 Publication
Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi113N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi157InterchainPROSITE-ProRule annotation
Modified residuei201Phosphotyrosine; by TXK and JAK2By similarity1

Post-translational modificationi

N-glycosylation is important for dimerization.By similarity
Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP09793
PaxDbiP09793
PRIDEiP09793

PTM databases

iPTMnetiP09793
PhosphoSitePlusiP09793

Expressioni

Tissue specificityi

Widely expressed with highest levels in lymphoid tissues.1 Publication

Gene expression databases

CleanExiMM_CTLA4

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Binds to CD80/B7-1 and CD86/B7.2. Interacts with ICOSLG.By similarity

Protein-protein interaction databases

BioGridi198575, 1 interactor
ELMiP09793
IntActiP09793, 1 interactor
MINTiP09793
STRINGi10090.ENSMUSP00000027164

Structurei

Secondary structure

1223
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP09793
SMRiP09793
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09793

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 145Ig-like V-typeAdd BLAST110

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 50HomodimerizationBy similarity5
Regioni134 – 139Important for interaction with CD80 and CD86By similarity6
Regioni150 – 155HomodimerizationBy similarity6

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJ05 Eukaryota
ENOG410YUQR LUCA
HOGENOMiHOG000112047
HOVERGENiHBG057978
InParanoidiP09793
KOiK06538
PhylomeDBiP09793

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR008096 CTLA4
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR013106 Ig_V-set
PANTHERiPTHR11494:SF8 PTHR11494:SF8, 1 hit
PfamiView protein in Pfam
PF07686 V-set, 1 hit
PRINTSiPR01720 CTLANTIGEN4
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00406 IGv, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P09793-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MACLGLRRYK AQLQLPSRTW PFVALLTLLF IPVFSEAIQV TQPSVVLASS
60 70 80 90 100
HGVASFPCEY SPSHNTDEVR VTVLRQTNDQ MTEVCATTFT EKNTVGFLDY
110 120 130 140 150
PFCSGTFNES RVNLTIQGLR AVDTGLYLCK VELMYPPPYF VGMGNGTQIY
160 170 180 190 200
VIDPEPCPDS DFLLWILVAV SLGLFFYSFL VSAVSLSKML KKRSPLTTGV
210 220
YVKMPPTEPE CEKQFQPYFI PIN
Length:223
Mass (Da):24,993
Last modified:July 1, 1989 - v1
Checksum:i5318FAAF416F4685
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q6GTR6Q6GTR6_MOUSE
Cytotoxic T-lymphocyte protein 4
Ctla4 CH29-120A16.2-001
223Annotation score:
Q5SSM0Q5SSM0_MOUSE
Cytotoxic T-lymphocyte protein 4
Ctla4 CH29-120A16.2-002
174Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182S → T in AAF01489 (PubMed:10493833).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05719 mRNA Translation: CAA29191.1
AF142145 Genomic DNA Translation: AAF01489.1
M74362 Genomic DNA Translation: AAA37489.1
CCDSiCCDS14993.1
PIRiA29063
RefSeqiNP_033973.2, NM_009843.4
UniGeneiMm.390

Genome annotation databases

GeneIDi12477
KEGGimmu:12477

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05719 mRNA Translation: CAA29191.1
AF142145 Genomic DNA Translation: AAF01489.1
M74362 Genomic DNA Translation: AAA37489.1
CCDSiCCDS14993.1
PIRiA29063
RefSeqiNP_033973.2, NM_009843.4
UniGeneiMm.390

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DQTX-ray2.00A/B/C/D38-154[»]
5E56X-ray1.50A38-154[»]
5E5MX-ray2.18A/C/E/G38-154[»]
ProteinModelPortaliP09793
SMRiP09793
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198575, 1 interactor
ELMiP09793
IntActiP09793, 1 interactor
MINTiP09793
STRINGi10090.ENSMUSP00000027164

PTM databases

iPTMnetiP09793
PhosphoSitePlusiP09793

Proteomic databases

EPDiP09793
PaxDbiP09793
PRIDEiP09793

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi12477
KEGGimmu:12477

Organism-specific databases

CTDi1493
MGIiMGI:88556 Ctla4

Phylogenomic databases

eggNOGiENOG410IJ05 Eukaryota
ENOG410YUQR LUCA
HOGENOMiHOG000112047
HOVERGENiHBG057978
InParanoidiP09793
KOiK06538
PhylomeDBiP09793

Miscellaneous databases

EvolutionaryTraceiP09793
PROiPR:P09793
SOURCEiSearch...

Gene expression databases

CleanExiMM_CTLA4

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR008096 CTLA4
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR013106 Ig_V-set
PANTHERiPTHR11494:SF8 PTHR11494:SF8, 1 hit
PfamiView protein in Pfam
PF07686 V-set, 1 hit
PRINTSiPR01720 CTLANTIGEN4
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00406 IGv, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCTLA4_MOUSE
AccessioniPrimary (citable) accession number: P09793
Secondary accession number(s): Q9QZZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 20, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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